Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contamination
To evaluate the application of cycle threshold (Ct) values of coronavirus disease 2019 (COVID-19) patients in predicting epidemic dynamics and monitoring surface contamination. The Ct value of reverse transcriptase-polymerase chain reaction for SARS‑CoV-2 from COVID-19 patients inbound overseas in X...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Infection and Public Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034122003057 |
| _version_ | 1811213864746876928 |
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| author | Yu-Yan Chen Xu Shen Yong-Jing Wang Jia-Wen Xie Zan-Xi Fang Li-Rong Lin Tian-Ci Yang |
| author_facet | Yu-Yan Chen Xu Shen Yong-Jing Wang Jia-Wen Xie Zan-Xi Fang Li-Rong Lin Tian-Ci Yang |
| author_sort | Yu-Yan Chen |
| collection | DOAJ |
| description | To evaluate the application of cycle threshold (Ct) values of coronavirus disease 2019 (COVID-19) patients in predicting epidemic dynamics and monitoring surface contamination. The Ct value of reverse transcriptase-polymerase chain reaction for SARS‑CoV-2 from COVID-19 patients inbound overseas in Xiamen, China was collected from October 2020 to December 2021, and the correlation of patients' Ct values with epidemic dynamics and surface contamination was evaluated. The results showed that there was an extreme inverse correlation of positivity rate in the current calendar month (ORF1ab, r = −0.692, P = 0.004; N,r = −0.629, P = 0.012) and the following calendar month (ORF1ab,r = −0.801, P = 0.001; N,r = −0.620, P = 0.018) with the median Ct values. Ct value showed better performance for monitoring surface contamination, with the area under the curve value 0.808(95 %CI: 0.748–0.869) for ORF1ab and 0.807(95 %CI:0.746–0.868) for the N gene. The patients’ ORF1ab Ct value< 29.09 or N Ct value< 28.03 were 11.25 times and 10.48 times more likely to result in surface contamination than those with ORF1ab Ct value ≥ 29.09 or N Ct value≥ 28.03 (OR:11.25,95 % CI: 5.52–22.35; OR:10.48,95 % CI:5.29–20.70). Ct values were associated with the positivity rate in the current or following calendar month and predicted the epidemic dynamics. The Ct values can be used as a predictor for monitoring surface contamination to develop public health responses to COVID-19. |
| first_indexed | 2024-04-12T05:53:25Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-04-12T05:53:25Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-f7702e98dec047df8659a5ee4f8a4d7a2022-12-22T03:45:13ZengElsevierJournal of Infection and Public Health1876-03412022-12-01151214941496Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contaminationYu-Yan Chen0Xu Shen1Yong-Jing Wang2Jia-Wen Xie3Zan-Xi Fang4Li-Rong Lin5Tian-Ci Yang6Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China; Department of Hospital Infection Management, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China; Xiamen Medical College, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China; Xiamen Medical College, Xiamen, ChinaCenter of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China; Xiamen Medical College, Xiamen, China; Corresponding authors at: Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China; Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, China; Corresponding authors at: Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.To evaluate the application of cycle threshold (Ct) values of coronavirus disease 2019 (COVID-19) patients in predicting epidemic dynamics and monitoring surface contamination. The Ct value of reverse transcriptase-polymerase chain reaction for SARS‑CoV-2 from COVID-19 patients inbound overseas in Xiamen, China was collected from October 2020 to December 2021, and the correlation of patients' Ct values with epidemic dynamics and surface contamination was evaluated. The results showed that there was an extreme inverse correlation of positivity rate in the current calendar month (ORF1ab, r = −0.692, P = 0.004; N,r = −0.629, P = 0.012) and the following calendar month (ORF1ab,r = −0.801, P = 0.001; N,r = −0.620, P = 0.018) with the median Ct values. Ct value showed better performance for monitoring surface contamination, with the area under the curve value 0.808(95 %CI: 0.748–0.869) for ORF1ab and 0.807(95 %CI:0.746–0.868) for the N gene. The patients’ ORF1ab Ct value< 29.09 or N Ct value< 28.03 were 11.25 times and 10.48 times more likely to result in surface contamination than those with ORF1ab Ct value ≥ 29.09 or N Ct value≥ 28.03 (OR:11.25,95 % CI: 5.52–22.35; OR:10.48,95 % CI:5.29–20.70). Ct values were associated with the positivity rate in the current or following calendar month and predicted the epidemic dynamics. The Ct values can be used as a predictor for monitoring surface contamination to develop public health responses to COVID-19.http://www.sciencedirect.com/science/article/pii/S1876034122003057COVID-19Cycle Threshold valueSARS-CoV-2Epidemic dynamicsSurface contaminationRT-qPCR |
| spellingShingle | Yu-Yan Chen Xu Shen Yong-Jing Wang Jia-Wen Xie Zan-Xi Fang Li-Rong Lin Tian-Ci Yang Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contamination Journal of Infection and Public Health COVID-19 Cycle Threshold value SARS-CoV-2 Epidemic dynamics Surface contamination RT-qPCR |
| title | Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contamination |
| title_full | Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contamination |
| title_fullStr | Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contamination |
| title_full_unstemmed | Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contamination |
| title_short | Evaluation of the cycle threshold values of RT-PCR for SARS-CoV-2 in COVID-19 patients in predicting epidemic dynamics and monitoring surface contamination |
| title_sort | evaluation of the cycle threshold values of rt pcr for sars cov 2 in covid 19 patients in predicting epidemic dynamics and monitoring surface contamination |
| topic | COVID-19 Cycle Threshold value SARS-CoV-2 Epidemic dynamics Surface contamination RT-qPCR |
| url | http://www.sciencedirect.com/science/article/pii/S1876034122003057 |
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