The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying Process
Lactic acid bacteria (LAB) and probiotics promise specific health benefits to their host. However, good storage stability is a prerequisite for their functioning and industrial use. This study aimed to evaluate glutathione (GSH) as a potential protective agent for improving microbial stability deter...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/2/187 |
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| author | Trung Hau Nguyen Jin-Seong Kim Hyuk-Ju Kwon Chang-Ho Kang |
| author_facet | Trung Hau Nguyen Jin-Seong Kim Hyuk-Ju Kwon Chang-Ho Kang |
| author_sort | Trung Hau Nguyen |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) and probiotics promise specific health benefits to their host. However, good storage stability is a prerequisite for their functioning and industrial use. This study aimed to evaluate glutathione (GSH) as a potential protective agent for improving microbial stability deteriorated by freeze-drying, freeze-thawing, and cold treatments. In this study, the optimal concentration of glutathione (50% <i>w</i>/<i>w</i>) was 1%, showing effective protection on the viability and stability of various LAB strains (<i>Lactiplantibacillus plantarum</i> MG4229 and MG4296, <i>Lactococcus lactis</i> MG5125, <i>Limosilactobacillus fermentum</i> MG4295, <i>Lacticaseibacillus paracasei</i> MG5012, and <i>Bifidobacterium animalis</i> ssp. <i>lactis</i> MG741). Glutathione-containing protectants considerably improved the viability of all of these strains after freeze-drying compared with non-coated probiotics. Survivability in the gastrointestinal (GI) tract, accelerated stability tests, and adhesion assays on intestinal epithelial cells were performed to determine whether glutathione enhances bacterial stability. Based on morphological observations, protectants containing GSH were coated onto the cell surface, resulting in effective protection against multiple external stress stimuli. The applicability of GSH as a new and effective protective agent can improve the stability and viability of various probiotics with anti-freezing and anti-thawing effects. |
| first_indexed | 2024-03-11T08:51:22Z |
| format | Article |
| id | doaj.art-f77099d300c14edbbfc1c3012f3517e8 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T08:51:22Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-f77099d300c14edbbfc1c3012f3517e82023-11-16T20:26:41ZengMDPI AGFermentation2311-56372023-02-019218710.3390/fermentation9020187The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying ProcessTrung Hau Nguyen0Jin-Seong Kim1Hyuk-Ju Kwon2Chang-Ho Kang3MEDIOGEN, Co., Ltd., Bio Valley 1-Ro, Jecheon-si 27159, Republic of KoreaMEDIOGEN, Co., Ltd., Bio Valley 1-Ro, Jecheon-si 27159, Republic of KoreaMEDIOGEN, Co., Ltd., Bio Valley 1-Ro, Jecheon-si 27159, Republic of KoreaMEDIOGEN, Co., Ltd., Bio Valley 1-Ro, Jecheon-si 27159, Republic of KoreaLactic acid bacteria (LAB) and probiotics promise specific health benefits to their host. However, good storage stability is a prerequisite for their functioning and industrial use. This study aimed to evaluate glutathione (GSH) as a potential protective agent for improving microbial stability deteriorated by freeze-drying, freeze-thawing, and cold treatments. In this study, the optimal concentration of glutathione (50% <i>w</i>/<i>w</i>) was 1%, showing effective protection on the viability and stability of various LAB strains (<i>Lactiplantibacillus plantarum</i> MG4229 and MG4296, <i>Lactococcus lactis</i> MG5125, <i>Limosilactobacillus fermentum</i> MG4295, <i>Lacticaseibacillus paracasei</i> MG5012, and <i>Bifidobacterium animalis</i> ssp. <i>lactis</i> MG741). Glutathione-containing protectants considerably improved the viability of all of these strains after freeze-drying compared with non-coated probiotics. Survivability in the gastrointestinal (GI) tract, accelerated stability tests, and adhesion assays on intestinal epithelial cells were performed to determine whether glutathione enhances bacterial stability. Based on morphological observations, protectants containing GSH were coated onto the cell surface, resulting in effective protection against multiple external stress stimuli. The applicability of GSH as a new and effective protective agent can improve the stability and viability of various probiotics with anti-freezing and anti-thawing effects.https://www.mdpi.com/2311-5637/9/2/187freeze-dryingglutathionegastrointestinal tractlactic acid bacteriaprobiotics |
| spellingShingle | Trung Hau Nguyen Jin-Seong Kim Hyuk-Ju Kwon Chang-Ho Kang The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying Process Fermentation freeze-drying glutathione gastrointestinal tract lactic acid bacteria probiotics |
| title | The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying Process |
| title_full | The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying Process |
| title_fullStr | The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying Process |
| title_full_unstemmed | The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying Process |
| title_short | The Effect of a Glutathione (GSH)-Containing Cryo-Protectant on the Viability of Probiotic Cells Using a Freeze-Drying Process |
| title_sort | effect of a glutathione gsh containing cryo protectant on the viability of probiotic cells using a freeze drying process |
| topic | freeze-drying glutathione gastrointestinal tract lactic acid bacteria probiotics |
| url | https://www.mdpi.com/2311-5637/9/2/187 |
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