Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter gene
ABSTRACTShigella flexneri causes severe diarrheal disease worldwide. While many aspects of pathogenesis have been elucidated, significant knowledge gaps remain regarding the role of putative chromosomally-encoded virulence genes. The uncharacterized sap gene encoded on the chromosome has significant...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2024.2331985 |
| _version_ | 1797235366967640064 |
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| author | Yrvin León Raphael Honigsberg David A. Rasko Christina S. Faherty |
| author_facet | Yrvin León Raphael Honigsberg David A. Rasko Christina S. Faherty |
| author_sort | Yrvin León |
| collection | DOAJ |
| description | ABSTRACTShigella flexneri causes severe diarrheal disease worldwide. While many aspects of pathogenesis have been elucidated, significant knowledge gaps remain regarding the role of putative chromosomally-encoded virulence genes. The uncharacterized sap gene encoded on the chromosome has significant nucleotide sequence identity to the fluffy (flu) antigen 43 autotransporter gene in pathogenic Escherichia coli. Here, we constructed a Δsap mutant in S. flexneri strain 2457T and examined the effects of this mutation on bacterial cell aggregation, biofilm formation, and adherence to colonic epithelial cells. Analyses included the use of growth media supplemented with glucose and bile salts to replicate small intestinal signals encountered by S. flexneri. Deletion of the sap gene in 2457T affected epithelial cell adherence, resulted in quicker bacterial cell aggregation, but did not affect biofilm formation. This work highlights a functional role for the sap gene in S. flexneri pathogenesis and further demonstrates the importance of using relevant and appropriate gastrointestinal signals to characterize virulence genes of enteropathogenic bacteria. |
| first_indexed | 2024-04-24T16:46:49Z |
| format | Article |
| id | doaj.art-f770f679e6b24637b53aea31630bf742 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-04-24T16:46:49Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-f770f679e6b24637b53aea31630bf7422024-03-29T06:56:16ZengTaylor & Francis GroupGut Microbes1949-09761949-09842024-12-0116110.1080/19490976.2024.2331985Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter geneYrvin León0Raphael Honigsberg1David A. Rasko2Christina S. Faherty3Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital, USAMucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital, USAInstitute for Genome Sciences, Center for Pathogen Research, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USAMucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital, USAABSTRACTShigella flexneri causes severe diarrheal disease worldwide. While many aspects of pathogenesis have been elucidated, significant knowledge gaps remain regarding the role of putative chromosomally-encoded virulence genes. The uncharacterized sap gene encoded on the chromosome has significant nucleotide sequence identity to the fluffy (flu) antigen 43 autotransporter gene in pathogenic Escherichia coli. Here, we constructed a Δsap mutant in S. flexneri strain 2457T and examined the effects of this mutation on bacterial cell aggregation, biofilm formation, and adherence to colonic epithelial cells. Analyses included the use of growth media supplemented with glucose and bile salts to replicate small intestinal signals encountered by S. flexneri. Deletion of the sap gene in 2457T affected epithelial cell adherence, resulted in quicker bacterial cell aggregation, but did not affect biofilm formation. This work highlights a functional role for the sap gene in S. flexneri pathogenesis and further demonstrates the importance of using relevant and appropriate gastrointestinal signals to characterize virulence genes of enteropathogenic bacteria.https://www.tandfonline.com/doi/10.1080/19490976.2024.2331985Shigella flexneriadherence factorsbile saltsglucoseautotransportersap |
| spellingShingle | Yrvin León Raphael Honigsberg David A. Rasko Christina S. Faherty Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter gene Gut Microbes Shigella flexneri adherence factors bile salts glucose autotransporter sap |
| title | Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter gene |
| title_full | Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter gene |
| title_fullStr | Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter gene |
| title_full_unstemmed | Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter gene |
| title_short | Gastrointestinal signals in supplemented media reveal a role in adherence for the Shigella flexneri sap autotransporter gene |
| title_sort | gastrointestinal signals in supplemented media reveal a role in adherence for the shigella flexneri sap autotransporter gene |
| topic | Shigella flexneri adherence factors bile salts glucose autotransporter sap |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2024.2331985 |
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