Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing
The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms o...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/11/5658 |
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| author | Beata Kaczmarek-Szczepańska Justyna Ostrowska Justyna Kozłowska Zofia Szota Anna A. Brożyna Rita Dreier Russel J. Reiter Andrzej T. Slominski Kerstin Steinbrink Konrad Kleszczyński |
| author_facet | Beata Kaczmarek-Szczepańska Justyna Ostrowska Justyna Kozłowska Zofia Szota Anna A. Brożyna Rita Dreier Russel J. Reiter Andrzej T. Slominski Kerstin Steinbrink Konrad Kleszczyński |
| author_sort | Beata Kaczmarek-Szczepańska |
| collection | DOAJ |
| description | The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and <i>Salmo salar</i> fish skin-derived collagens to assess biophysical and cellular properties by (<i>i</i>) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (<i>ii</i>) thermogravimetric analysis (TG), (<i>iii</i>) scanning electron microscope (SEM), and (<i>iv</i>) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine. |
| first_indexed | 2024-03-10T11:02:00Z |
| format | Article |
| id | doaj.art-f7716c1cc60043a889e10f216620025f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:02:00Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f7716c1cc60043a889e10f216620025f2023-11-21T21:27:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012211565810.3390/ijms22115658Evaluation of Polymeric Matrix Loaded with Melatonin for Wound DressingBeata Kaczmarek-Szczepańska0Justyna Ostrowska1Justyna Kozłowska2Zofia Szota3Anna A. Brożyna4Rita Dreier5Russel J. Reiter6Andrzej T. Slominski7Kerstin Steinbrink8Konrad Kleszczyński9Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, PolandDepartment of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, PolandDepartment of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, PolandDepartment of Human Biology, Institute of Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandDepartment of Human Biology, Institute of Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandInstitute of Physiological Chemistry and Pathobiochemistry, Waldeyerstraße 15, 48149 Münster, GermanyDepartment of Cellular and Structural Biology, UT Health Science Center, San Antonio, TX 78229, USAComprehensive Cancer Center, Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, GermanyDepartment of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, GermanyThe development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and <i>Salmo salar</i> fish skin-derived collagens to assess biophysical and cellular properties by (<i>i</i>) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (<i>ii</i>) thermogravimetric analysis (TG), (<i>iii</i>) scanning electron microscope (SEM), and (<i>iv</i>) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine.https://www.mdpi.com/1422-0067/22/11/5658melatoninscaffoldsbiopolymerscollagenchitosancutaneous cells |
| spellingShingle | Beata Kaczmarek-Szczepańska Justyna Ostrowska Justyna Kozłowska Zofia Szota Anna A. Brożyna Rita Dreier Russel J. Reiter Andrzej T. Slominski Kerstin Steinbrink Konrad Kleszczyński Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing International Journal of Molecular Sciences melatonin scaffolds biopolymers collagen chitosan cutaneous cells |
| title | Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
| title_full | Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
| title_fullStr | Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
| title_full_unstemmed | Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
| title_short | Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing |
| title_sort | evaluation of polymeric matrix loaded with melatonin for wound dressing |
| topic | melatonin scaffolds biopolymers collagen chitosan cutaneous cells |
| url | https://www.mdpi.com/1422-0067/22/11/5658 |
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