Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake
Remediation using micro-algae offers an attractive solution to environmental phosphate (PO43-) pollution. However, for maximum efficiency, pre-conditioning of algae to induce ‘luxury phosphorus (P) uptake’ is needed. To replicate this process, we targeted the global regulator PSR1 (Myb transcription...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1208168/full |
| _version_ | 1797771130725990400 |
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| author | Stephen P. Slocombe Tatiana Zúñiga-Burgos Tatiana Zúñiga-Burgos Lili Chu Payam Mehrshahi Matthew P. Davey Alison G. Smith Miller Alonso Camargo-Valero Miller Alonso Camargo-Valero Alison Baker |
| author_facet | Stephen P. Slocombe Tatiana Zúñiga-Burgos Tatiana Zúñiga-Burgos Lili Chu Payam Mehrshahi Matthew P. Davey Alison G. Smith Miller Alonso Camargo-Valero Miller Alonso Camargo-Valero Alison Baker |
| author_sort | Stephen P. Slocombe |
| collection | DOAJ |
| description | Remediation using micro-algae offers an attractive solution to environmental phosphate (PO43-) pollution. However, for maximum efficiency, pre-conditioning of algae to induce ‘luxury phosphorus (P) uptake’ is needed. To replicate this process, we targeted the global regulator PSR1 (Myb transcription factor: Phosphate Starvation Response 1) for over-expression in algae. Manipulating a single gene (PSR1) drove uptake of both PO43- and a Mg2+ counter-ion leading to increased PolyP granule size, raising P levels 4-fold to 8% dry cell weight, and accelerated removal of PO43- from the medium. Examination of the gene expression profile showed that the P-starvation response was mimicked under P-replete conditions, switching on luxury uptake. Hyper-accumulation of P depended on a feed-forward mechanism, where a small set of ‘Class I’ P-transporter genes were activated despite abundant external PO43- levels. The transporters drove a reduction in external PO43- levels, permitting more genes to be expressed (Class II), leading to more P-uptake. Our data pointed toward a PSR1-independent mechanism for detection of external PO43- which suppressed Class II genes. This model provided a plausible mechanism for P-overplus where prior P-starvation elevates PSR1 and on P-resupply causes luxury P-uptake. This is because the Class I genes, which include P-transporter genes, are not suppressed by the excess PO43-. Taken together, these discoveries facilitate a bio-circular approach of recycling nutrients from wastewater back to agriculture. |
| first_indexed | 2024-03-12T21:33:05Z |
| format | Article |
| id | doaj.art-f773281e1b1543bbb29928bb48941362 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T21:33:05Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f773281e1b1543bbb29928bb489413622023-07-27T16:44:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.12081681208168Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptakeStephen P. Slocombe0Tatiana Zúñiga-Burgos1Tatiana Zúñiga-Burgos2Lili Chu3Payam Mehrshahi4Matthew P. Davey5Alison G. Smith6Miller Alonso Camargo-Valero7Miller Alonso Camargo-Valero8Alison Baker9School of Molecular and Cellular Biology, Centre for Plant Sciences and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United KingdomSchool of Molecular and Cellular Biology, Centre for Plant Sciences and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United KingdomBioResource Systems Research Group, School of Civil Engineering, University of Leeds, Leeds, United KingdomSchool of Molecular and Cellular Biology, Centre for Plant Sciences and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United KingdomDepartment of Plant Sciences, Cambridge University, Cambridge, United KingdomDepartment of Plant Sciences, Cambridge University, Cambridge, United KingdomDepartment of Plant Sciences, Cambridge University, Cambridge, United KingdomBioResource Systems Research Group, School of Civil Engineering, University of Leeds, Leeds, United KingdomDepartamento de Ingeniería Química, Universidad Nacional de Colombia, Manizales, ColombiaSchool of Molecular and Cellular Biology, Centre for Plant Sciences and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United KingdomRemediation using micro-algae offers an attractive solution to environmental phosphate (PO43-) pollution. However, for maximum efficiency, pre-conditioning of algae to induce ‘luxury phosphorus (P) uptake’ is needed. To replicate this process, we targeted the global regulator PSR1 (Myb transcription factor: Phosphate Starvation Response 1) for over-expression in algae. Manipulating a single gene (PSR1) drove uptake of both PO43- and a Mg2+ counter-ion leading to increased PolyP granule size, raising P levels 4-fold to 8% dry cell weight, and accelerated removal of PO43- from the medium. Examination of the gene expression profile showed that the P-starvation response was mimicked under P-replete conditions, switching on luxury uptake. Hyper-accumulation of P depended on a feed-forward mechanism, where a small set of ‘Class I’ P-transporter genes were activated despite abundant external PO43- levels. The transporters drove a reduction in external PO43- levels, permitting more genes to be expressed (Class II), leading to more P-uptake. Our data pointed toward a PSR1-independent mechanism for detection of external PO43- which suppressed Class II genes. This model provided a plausible mechanism for P-overplus where prior P-starvation elevates PSR1 and on P-resupply causes luxury P-uptake. This is because the Class I genes, which include P-transporter genes, are not suppressed by the excess PO43-. Taken together, these discoveries facilitate a bio-circular approach of recycling nutrients from wastewater back to agriculture.https://www.frontiersin.org/articles/10.3389/fpls.2023.1208168/fullbiomassmicro-algaepolyphosphatetranscription factorwastewater remediation |
| spellingShingle | Stephen P. Slocombe Tatiana Zúñiga-Burgos Tatiana Zúñiga-Burgos Lili Chu Payam Mehrshahi Matthew P. Davey Alison G. Smith Miller Alonso Camargo-Valero Miller Alonso Camargo-Valero Alison Baker Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake Frontiers in Plant Science biomass micro-algae polyphosphate transcription factor wastewater remediation |
| title | Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake |
| title_full | Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake |
| title_fullStr | Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake |
| title_full_unstemmed | Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake |
| title_short | Overexpression of PSR1 in Chlamydomonas reinhardtii induces luxury phosphorus uptake |
| title_sort | overexpression of psr1 in chlamydomonas reinhardtii induces luxury phosphorus uptake |
| topic | biomass micro-algae polyphosphate transcription factor wastewater remediation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1208168/full |
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