The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i>
The fungus <i>Monilinia fructicola</i> is responsible for brown rot on stone and pome fruit and causes heavy yield losses both pre- and post-harvest. Several mycoviruses are known to infect fungal plant pathogens. In this study, a metagenomic approach was applied to obtain a comprehensiv...
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MDPI AG
2022-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/5/481 |
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| author | Rita Milvia De Miccolis Angelini Celeste Raguseo Caterina Rotolo Donato Gerin Francesco Faretra Stefania Pollastro |
| author_facet | Rita Milvia De Miccolis Angelini Celeste Raguseo Caterina Rotolo Donato Gerin Francesco Faretra Stefania Pollastro |
| author_sort | Rita Milvia De Miccolis Angelini |
| collection | DOAJ |
| description | The fungus <i>Monilinia fructicola</i> is responsible for brown rot on stone and pome fruit and causes heavy yield losses both pre- and post-harvest. Several mycoviruses are known to infect fungal plant pathogens. In this study, a metagenomic approach was applied to obtain a comprehensive characterization of the mycovirome in a worldwide collection of 58 <i>M. fructicola</i> strains. Deep sequencing of double-stranded (ds)RNA extracts revealed a great abundance and variety of mycoviruses. A total of 32 phylogenetically distinct positive-sense (+) single-stranded (ss)RNA viruses were identified. They included twelve mitoviruses, one in the proposed family <i>Splipalmiviridae</i>, and twelve botourmiaviruses (phylum <i>Lenarviricota</i>), eleven of which were novel viral species; two hypoviruses, three in the proposed family <i>Fusariviridae</i>, and one barnavirus (phylum <i>Pisuviricota</i>); as well as one novel beny-like virus (phylum <i>Kitrinoviricota</i>), the first one identified in Ascomycetes. A partial sequence of a new putative ssDNA mycovirus related to viruses within the <i>Parvoviridae</i> family was detected in a <i>M. fructicola</i> isolate from Serbia. The availability of genomic sequences of mycoviruses will serve as a solid basis for further research aimed at deepening the knowledge on virus–host and virus–virus interactions and to explore their potential as biocontrol agents against brown rot disease. |
| first_indexed | 2024-03-10T03:38:11Z |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T03:38:11Z |
| publishDate | 2022-05-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-f77450d0b5404c9f8f340311df336d7c2023-11-23T11:41:45ZengMDPI AGJournal of Fungi2309-608X2022-05-018548110.3390/jof8050481The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i>Rita Milvia De Miccolis Angelini0Celeste Raguseo1Caterina Rotolo2Donato Gerin3Francesco Faretra4Stefania Pollastro5Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, 70126 Bari, ItalyThe fungus <i>Monilinia fructicola</i> is responsible for brown rot on stone and pome fruit and causes heavy yield losses both pre- and post-harvest. Several mycoviruses are known to infect fungal plant pathogens. In this study, a metagenomic approach was applied to obtain a comprehensive characterization of the mycovirome in a worldwide collection of 58 <i>M. fructicola</i> strains. Deep sequencing of double-stranded (ds)RNA extracts revealed a great abundance and variety of mycoviruses. A total of 32 phylogenetically distinct positive-sense (+) single-stranded (ss)RNA viruses were identified. They included twelve mitoviruses, one in the proposed family <i>Splipalmiviridae</i>, and twelve botourmiaviruses (phylum <i>Lenarviricota</i>), eleven of which were novel viral species; two hypoviruses, three in the proposed family <i>Fusariviridae</i>, and one barnavirus (phylum <i>Pisuviricota</i>); as well as one novel beny-like virus (phylum <i>Kitrinoviricota</i>), the first one identified in Ascomycetes. A partial sequence of a new putative ssDNA mycovirus related to viruses within the <i>Parvoviridae</i> family was detected in a <i>M. fructicola</i> isolate from Serbia. The availability of genomic sequences of mycoviruses will serve as a solid basis for further research aimed at deepening the knowledge on virus–host and virus–virus interactions and to explore their potential as biocontrol agents against brown rot disease.https://www.mdpi.com/2309-608X/8/5/481mycovirusstone fruit+ssRNA virusmitovirussplipalmivirusbotourmiavirus |
| spellingShingle | Rita Milvia De Miccolis Angelini Celeste Raguseo Caterina Rotolo Donato Gerin Francesco Faretra Stefania Pollastro The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i> Journal of Fungi mycovirus stone fruit +ssRNA virus mitovirus splipalmivirus botourmiavirus |
| title | The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i> |
| title_full | The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i> |
| title_fullStr | The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i> |
| title_full_unstemmed | The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i> |
| title_short | The Mycovirome in a Worldwide Collection of the Brown Rot Fungus <i>Monilinia fructicola</i> |
| title_sort | mycovirome in a worldwide collection of the brown rot fungus i monilinia fructicola i |
| topic | mycovirus stone fruit +ssRNA virus mitovirus splipalmivirus botourmiavirus |
| url | https://www.mdpi.com/2309-608X/8/5/481 |
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