Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligands
In the present work, nineteen analogues of 1-[(2,2-Diphenyl-1,3-dioxolan-4-yl)methyl]-4-(2-methoxyphenyl)piperazine 5 and N-[2-(2-Methoxyphenoxy)ethyl]-2,2-diphenyl-1,3-dioxolane-4-methanamine 18 were synthesized. The compounds were tested for binding affinity at 5-HT1AR and α1-AR subtypes. They wer...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2022-01-01
|
Series: | Results in Chemistry |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715622001448 |
_version_ | 1811178095260991488 |
---|---|
author | Claudia Sorbi Silvia Franchini Michela Buccioni Antonio Cilia Lorenza Pirona Livio Brasili |
author_facet | Claudia Sorbi Silvia Franchini Michela Buccioni Antonio Cilia Lorenza Pirona Livio Brasili |
author_sort | Claudia Sorbi |
collection | DOAJ |
description | In the present work, nineteen analogues of 1-[(2,2-Diphenyl-1,3-dioxolan-4-yl)methyl]-4-(2-methoxyphenyl)piperazine 5 and N-[2-(2-Methoxyphenoxy)ethyl]-2,2-diphenyl-1,3-dioxolane-4-methanamine 18 were synthesized. The compounds were tested for binding affinity at 5-HT1AR and α1-AR subtypes. They were also tested using functional assays as α1-AR antagonists and the most promising were tested for functional activity at 5-HT1AR, where they were shown to behave as agonists. The results highlight that the replacement of the 1,3-dioxolane ring with a 1,3-oxathiolane or a 1,3-dithiolane moiety leads to an overall reduction in in-vitro affinity at the α1-AR, while affinity, potency and efficacy were strongly enhanced at the 5-HT1A receptor. Overall, the nitrogen-containing aromatic moieties scarcely affect the affinity at the 5-HT1A receptor, while reducing potency and increasing efficacy. The oxidation of the sulphur atom in the 1,3-oxathiolane to give sulfoxides and solfones has a negative effect on affinity and potency at both receptor systems. Regardless of the effect on the other parameters, selectivity toward 5-HT1AR with respect to the α1-AR is often favoured, but never the contrary. The most striking result is the inversion of selectivity. In fact, while the lead 5 is 100-fold selective for α1-AR, the new derivatives, although to differing degrees, are selective for 5-HT1AR. |
first_indexed | 2024-04-11T06:13:41Z |
format | Article |
id | doaj.art-f777a42c520f4bb4b5fc5bc58cced3b8 |
institution | Directory Open Access Journal |
issn | 2211-7156 |
language | English |
last_indexed | 2024-04-11T06:13:41Z |
publishDate | 2022-01-01 |
publisher | Elsevier |
record_format | Article |
series | Results in Chemistry |
spelling | doaj.art-f777a42c520f4bb4b5fc5bc58cced3b82022-12-22T04:41:09ZengElsevierResults in Chemistry2211-71562022-01-014100425Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligandsClaudia Sorbi0Silvia Franchini1Michela Buccioni2Antonio Cilia3Lorenza Pirona4Livio Brasili5Dipartimento di Scienze della Vita, Università degli Studi di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, ItalyDipartimento di Scienze della Vita, Università degli Studi di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, ItalyScuola di Scienze del Farmaco e dei Prodotti della Salute, Università di Camerino, Via S. Agostino 1, 62032 Camerino, ItalyDivisione Ricerca e Sviluppo, Recordati S.p.A., Via Civitali 1, 20148 Milano, ItalyDivisione Ricerca e Sviluppo, Recordati S.p.A., Via Civitali 1, 20148 Milano, ItalyDipartimento di Scienze della Vita, Università degli Studi di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, Italy; Corresponding author.In the present work, nineteen analogues of 1-[(2,2-Diphenyl-1,3-dioxolan-4-yl)methyl]-4-(2-methoxyphenyl)piperazine 5 and N-[2-(2-Methoxyphenoxy)ethyl]-2,2-diphenyl-1,3-dioxolane-4-methanamine 18 were synthesized. The compounds were tested for binding affinity at 5-HT1AR and α1-AR subtypes. They were also tested using functional assays as α1-AR antagonists and the most promising were tested for functional activity at 5-HT1AR, where they were shown to behave as agonists. The results highlight that the replacement of the 1,3-dioxolane ring with a 1,3-oxathiolane or a 1,3-dithiolane moiety leads to an overall reduction in in-vitro affinity at the α1-AR, while affinity, potency and efficacy were strongly enhanced at the 5-HT1A receptor. Overall, the nitrogen-containing aromatic moieties scarcely affect the affinity at the 5-HT1A receptor, while reducing potency and increasing efficacy. The oxidation of the sulphur atom in the 1,3-oxathiolane to give sulfoxides and solfones has a negative effect on affinity and potency at both receptor systems. Regardless of the effect on the other parameters, selectivity toward 5-HT1AR with respect to the α1-AR is often favoured, but never the contrary. The most striking result is the inversion of selectivity. In fact, while the lead 5 is 100-fold selective for α1-AR, the new derivatives, although to differing degrees, are selective for 5-HT1AR.http://www.sciencedirect.com/science/article/pii/S2211715622001448PiperazineAryloxyethylamineα1-Receptor5-HT1A receptor |
spellingShingle | Claudia Sorbi Silvia Franchini Michela Buccioni Antonio Cilia Lorenza Pirona Livio Brasili Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligands Results in Chemistry Piperazine Aryloxyethylamine α1-Receptor 5-HT1A receptor |
title | Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligands |
title_full | Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligands |
title_fullStr | Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligands |
title_full_unstemmed | Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligands |
title_short | Effect of the replacement of the o-methoxyphenyl moiety with nitrogen-containing aromatic rings within N-phenyl-piperazine and phenoxy-ethylamine-based 1,3-dioxo/oxathio/dithiolanes as α1 and 5-HT1A receptor ligands |
title_sort | effect of the replacement of the o methoxyphenyl moiety with nitrogen containing aromatic rings within n phenyl piperazine and phenoxy ethylamine based 1 3 dioxo oxathio dithiolanes as α1 and 5 ht1a receptor ligands |
topic | Piperazine Aryloxyethylamine α1-Receptor 5-HT1A receptor |
url | http://www.sciencedirect.com/science/article/pii/S2211715622001448 |
work_keys_str_mv | AT claudiasorbi effectofthereplacementoftheomethoxyphenylmoietywithnitrogencontainingaromaticringswithinnphenylpiperazineandphenoxyethylaminebased13dioxooxathiodithiolanesasa1and5ht1areceptorligands AT silviafranchini effectofthereplacementoftheomethoxyphenylmoietywithnitrogencontainingaromaticringswithinnphenylpiperazineandphenoxyethylaminebased13dioxooxathiodithiolanesasa1and5ht1areceptorligands AT michelabuccioni effectofthereplacementoftheomethoxyphenylmoietywithnitrogencontainingaromaticringswithinnphenylpiperazineandphenoxyethylaminebased13dioxooxathiodithiolanesasa1and5ht1areceptorligands AT antoniocilia effectofthereplacementoftheomethoxyphenylmoietywithnitrogencontainingaromaticringswithinnphenylpiperazineandphenoxyethylaminebased13dioxooxathiodithiolanesasa1and5ht1areceptorligands AT lorenzapirona effectofthereplacementoftheomethoxyphenylmoietywithnitrogencontainingaromaticringswithinnphenylpiperazineandphenoxyethylaminebased13dioxooxathiodithiolanesasa1and5ht1areceptorligands AT liviobrasili effectofthereplacementoftheomethoxyphenylmoietywithnitrogencontainingaromaticringswithinnphenylpiperazineandphenoxyethylaminebased13dioxooxathiodithiolanesasa1and5ht1areceptorligands |