Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing.
Various strategies have been attempted to replace esophageal defects with natural or artificial substitutes using tissue engineering. However, these methods have not yet reached clinical application because of the high risks related to their immunogenicity or insufficient biocompatibility. In this s...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0211339 |
| _version_ | 1818678548155596800 |
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| author | Yosuke Takeoka Keitaro Matsumoto Daisuke Taniguchi Tomoshi Tsuchiya Ryusuke Machino Masaaki Moriyama Shosaburo Oyama Tomoyuki Tetsuo Yasuaki Taura Katsunori Takagi Takuya Yoshida Abdelmotagaly Elgalad Naoto Matsuo Masaki Kunizaki Shuichi Tobinaga Takashi Nonaka Shigekazu Hidaka Naoya Yamasaki Koichi Nakayama Takeshi Nagayasu |
| author_facet | Yosuke Takeoka Keitaro Matsumoto Daisuke Taniguchi Tomoshi Tsuchiya Ryusuke Machino Masaaki Moriyama Shosaburo Oyama Tomoyuki Tetsuo Yasuaki Taura Katsunori Takagi Takuya Yoshida Abdelmotagaly Elgalad Naoto Matsuo Masaki Kunizaki Shuichi Tobinaga Takashi Nonaka Shigekazu Hidaka Naoya Yamasaki Koichi Nakayama Takeshi Nagayasu |
| author_sort | Yosuke Takeoka |
| collection | DOAJ |
| description | Various strategies have been attempted to replace esophageal defects with natural or artificial substitutes using tissue engineering. However, these methods have not yet reached clinical application because of the high risks related to their immunogenicity or insufficient biocompatibility. In this study, we developed a scaffold-free structure with a mixture of cell types using bio-three-dimensional (3D) printing technology and assessed its characteristics in vitro and in vivo after transplantation into rats. Normal human dermal fibroblasts, human esophageal smooth muscle cells, human bone marrow-derived mesenchymal stem cells, and human umbilical vein endothelial cells were purchased and used as a cell source. After the preparation of multicellular spheroids, esophageal-like tube structures were prepared by bio-3D printing. The structures were matured in a bioreactor and transplanted into 10-12-week-old F344 male rats as esophageal grafts under general anesthesia. Mechanical and histochemical assessment of the structures were performed. Among 4 types of structures evaluated, those with the larger proportion of mesenchymal stem cells tended to show greater strength and expansion on mechanical testing and highly expressed α-smooth muscle actin and vascular endothelial growth factor on immunohistochemistry. Therefore, the structure with the larger proportion of mesenchymal stem cells was selected for transplantation. The scaffold-free structures had sufficient strength for transplantation between the esophagus and stomach using silicon stents. The structures were maintained in vivo for 30 days after transplantation. Smooth muscle cells were maintained, and flat epithelium extended and covered the inner surface of the lumen. Food had also passed through the structure. These results suggested that the esophagus-like scaffold-free tubular structures created using bio-3D printing could hold promise as a substitute for the repair of esophageal defects. |
| first_indexed | 2024-12-17T09:17:01Z |
| format | Article |
| id | doaj.art-f77ce61bd48347c493aa9e0cd61d2b4a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T09:17:01Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f77ce61bd48347c493aa9e0cd61d2b4a2022-12-21T21:54:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01143e021133910.1371/journal.pone.0211339Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing.Yosuke TakeokaKeitaro MatsumotoDaisuke TaniguchiTomoshi TsuchiyaRyusuke MachinoMasaaki MoriyamaShosaburo OyamaTomoyuki TetsuoYasuaki TauraKatsunori TakagiTakuya YoshidaAbdelmotagaly ElgaladNaoto MatsuoMasaki KunizakiShuichi TobinagaTakashi NonakaShigekazu HidakaNaoya YamasakiKoichi NakayamaTakeshi NagayasuVarious strategies have been attempted to replace esophageal defects with natural or artificial substitutes using tissue engineering. However, these methods have not yet reached clinical application because of the high risks related to their immunogenicity or insufficient biocompatibility. In this study, we developed a scaffold-free structure with a mixture of cell types using bio-three-dimensional (3D) printing technology and assessed its characteristics in vitro and in vivo after transplantation into rats. Normal human dermal fibroblasts, human esophageal smooth muscle cells, human bone marrow-derived mesenchymal stem cells, and human umbilical vein endothelial cells were purchased and used as a cell source. After the preparation of multicellular spheroids, esophageal-like tube structures were prepared by bio-3D printing. The structures were matured in a bioreactor and transplanted into 10-12-week-old F344 male rats as esophageal grafts under general anesthesia. Mechanical and histochemical assessment of the structures were performed. Among 4 types of structures evaluated, those with the larger proportion of mesenchymal stem cells tended to show greater strength and expansion on mechanical testing and highly expressed α-smooth muscle actin and vascular endothelial growth factor on immunohistochemistry. Therefore, the structure with the larger proportion of mesenchymal stem cells was selected for transplantation. The scaffold-free structures had sufficient strength for transplantation between the esophagus and stomach using silicon stents. The structures were maintained in vivo for 30 days after transplantation. Smooth muscle cells were maintained, and flat epithelium extended and covered the inner surface of the lumen. Food had also passed through the structure. These results suggested that the esophagus-like scaffold-free tubular structures created using bio-3D printing could hold promise as a substitute for the repair of esophageal defects.https://doi.org/10.1371/journal.pone.0211339 |
| spellingShingle | Yosuke Takeoka Keitaro Matsumoto Daisuke Taniguchi Tomoshi Tsuchiya Ryusuke Machino Masaaki Moriyama Shosaburo Oyama Tomoyuki Tetsuo Yasuaki Taura Katsunori Takagi Takuya Yoshida Abdelmotagaly Elgalad Naoto Matsuo Masaki Kunizaki Shuichi Tobinaga Takashi Nonaka Shigekazu Hidaka Naoya Yamasaki Koichi Nakayama Takeshi Nagayasu Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing. PLoS ONE |
| title | Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing. |
| title_full | Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing. |
| title_fullStr | Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing. |
| title_full_unstemmed | Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing. |
| title_short | Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing. |
| title_sort | regeneration of esophagus using a scaffold free biomimetic structure created with bio three dimensional printing |
| url | https://doi.org/10.1371/journal.pone.0211339 |
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