Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma
Objective: lymph node metastases occur in 90% of cases in patients with gastric adenocarcinoma, and survival is less than 15% in the late stages of disease. Therefore, the purpose of the study was to screening markers for predicting the likelihood of metastases developing in gastric adenocarcinoma....
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| Format: | Article |
| Language: | English |
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State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation
2018-03-01
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| Series: | Медицинский вестник Юга России |
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| Online Access: | https://www.medicalherald.ru/jour/article/view/636 |
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| author | O. I. Kit D. S. Kutilin M. Z. Tatimov A. A. Maslov E. M. Frantsiyants D. I. Vodolazhsky |
| author_facet | O. I. Kit D. S. Kutilin M. Z. Tatimov A. A. Maslov E. M. Frantsiyants D. I. Vodolazhsky |
| author_sort | O. I. Kit |
| collection | DOAJ |
| description | Objective: lymph node metastases occur in 90% of cases in patients with gastric adenocarcinoma, and survival is less than 15% in the late stages of disease. Therefore, the purpose of the study was to screening markers for predicting the likelihood of metastases developing in gastric adenocarcinoma. Materials and methods: for the study 47 patients with diagnosis of gastric adenocarcinoma were used (with the localized tumor form T3-4N0M0 (n = 18) and the locally distributed form T3-4N1-3M0 (n = 29)). The copy number variation of 16 genes (APC, AURKA, CCND1, C-MYC, GKN1, HER2, IRX1, MDM2, MET, NFKB, OCT1, P53, PIK3CA, POU5F1B, S6K2 and HV2) was determined by RT-PCR. Immunohistochemical studies were performed on sections of paraffin blocks using monoclonal antibodies to p53, Bcl-2, Ki-67 and Her2. Results: the loss of duplicity of HV2 locus was most frequent CNV in samples of stomach adenocarcinoma, but CNV significant change in the T3-4N0M0 group relative to T3-4N1-3M0 was not detected. A significant increase of CNV in the T3-4N1-3M0 group relative to T3-4N0M0 was found in 2 of 16 loci - NFKB and HER2 in 1.75 and 1.90 times (p<0.0005), respectively. Th e value of p53 expression in the tumor cells nuclei in the group without metastases was lower by 52% compared to the expression in the metastatic group (p<0.05). Low proliferative activity prevailed in the group without metastases in contrast to the group with metastases, in which Her2 overexpression was also observed in 43% of cases in contrast to 20% of cases in the group without metastases. Conclusions: it was showed that the relative copies number of NFKB1 and HER2 in combination with the immunohistochemical parameters of Ki67 and HER2 in gastric tumors can be a prognostic marker of metastasis, and algorithm for predicting metastases development in gastric adenocarcinoma is formed on basis of this. |
| first_indexed | 2024-04-11T14:32:36Z |
| format | Article |
| id | doaj.art-f77e9518337a41f596e16891f1b99ded |
| institution | Directory Open Access Journal |
| issn | 2219-8075 2618-7876 |
| language | English |
| last_indexed | 2024-04-11T14:32:36Z |
| publishDate | 2018-03-01 |
| publisher | State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation |
| record_format | Article |
| series | Медицинский вестник Юга России |
| spelling | doaj.art-f77e9518337a41f596e16891f1b99ded2022-12-22T04:18:29ZengState Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian FederationМедицинский вестник Юга России2219-80752618-78762018-03-0191516210.21886/2219-8075-2018-9-1-51-62431Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinomaO. I. Kit0D. S. Kutilin1M. Z. Tatimov2A. A. Maslov3E. M. Frantsiyants4D. I. Vodolazhsky5Rostov Research Institute of Oncology, Rostov-on-DonRostov Research Institute of Oncology, Rostov-on-DonRostov Research Institute of Oncology, Rostov-on-DonRostov Research Institute of Oncology, Rostov-on-DonRostov Research Institute of Oncology, Rostov-on-DonRostov Research Institute of Oncology, Rostov-on-DonObjective: lymph node metastases occur in 90% of cases in patients with gastric adenocarcinoma, and survival is less than 15% in the late stages of disease. Therefore, the purpose of the study was to screening markers for predicting the likelihood of metastases developing in gastric adenocarcinoma. Materials and methods: for the study 47 patients with diagnosis of gastric adenocarcinoma were used (with the localized tumor form T3-4N0M0 (n = 18) and the locally distributed form T3-4N1-3M0 (n = 29)). The copy number variation of 16 genes (APC, AURKA, CCND1, C-MYC, GKN1, HER2, IRX1, MDM2, MET, NFKB, OCT1, P53, PIK3CA, POU5F1B, S6K2 and HV2) was determined by RT-PCR. Immunohistochemical studies were performed on sections of paraffin blocks using monoclonal antibodies to p53, Bcl-2, Ki-67 and Her2. Results: the loss of duplicity of HV2 locus was most frequent CNV in samples of stomach adenocarcinoma, but CNV significant change in the T3-4N0M0 group relative to T3-4N1-3M0 was not detected. A significant increase of CNV in the T3-4N1-3M0 group relative to T3-4N0M0 was found in 2 of 16 loci - NFKB and HER2 in 1.75 and 1.90 times (p<0.0005), respectively. Th e value of p53 expression in the tumor cells nuclei in the group without metastases was lower by 52% compared to the expression in the metastatic group (p<0.05). Low proliferative activity prevailed in the group without metastases in contrast to the group with metastases, in which Her2 overexpression was also observed in 43% of cases in contrast to 20% of cases in the group without metastases. Conclusions: it was showed that the relative copies number of NFKB1 and HER2 in combination with the immunohistochemical parameters of Ki67 and HER2 in gastric tumors can be a prognostic marker of metastasis, and algorithm for predicting metastases development in gastric adenocarcinoma is formed on basis of this.https://www.medicalherald.ru/jour/article/view/636metastasesgene copy number variationproliferative activitygastric adenocarcinoma |
| spellingShingle | O. I. Kit D. S. Kutilin M. Z. Tatimov A. A. Maslov E. M. Frantsiyants D. I. Vodolazhsky Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma Медицинский вестник Юга России metastases gene copy number variation proliferative activity gastric adenocarcinoma |
| title | Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma |
| title_full | Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma |
| title_fullStr | Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma |
| title_full_unstemmed | Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma |
| title_short | Predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma |
| title_sort | predictive diagnostics of lymph nodes metastatic lesion in patients with gastric adenocarcinoma |
| topic | metastases gene copy number variation proliferative activity gastric adenocarcinoma |
| url | https://www.medicalherald.ru/jour/article/view/636 |
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