Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone Regeneration
Abstract Although artificial bone repair scaffolds, such as titanium alloy, bioactive glass, and hydroxyapatite (HAp), have been widely used for treatment of large‐size bone defects or serious bone destruction, they normally exhibit unsatisfied bone repair efficiency because of their weak osteogenic...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202204234 |
| _version_ | 1797962108141305856 |
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| author | Guanyun Wang Zehui Lv Tao Wang Tingting Hu Yixin Bian Yu Yang Ruizheng Liang Chaoliang Tan Xisheng Weng |
| author_facet | Guanyun Wang Zehui Lv Tao Wang Tingting Hu Yixin Bian Yu Yang Ruizheng Liang Chaoliang Tan Xisheng Weng |
| author_sort | Guanyun Wang |
| collection | DOAJ |
| description | Abstract Although artificial bone repair scaffolds, such as titanium alloy, bioactive glass, and hydroxyapatite (HAp), have been widely used for treatment of large‐size bone defects or serious bone destruction, they normally exhibit unsatisfied bone repair efficiency because of their weak osteogenic and angiogenesis performance as well as poor cell crawling and adhesion properties. Herein, the surface functionalization of MgAlEu‐layered double hydroxide (MAE‐LDH) nanosheets on porous HAp scaffolds is reported as a simple and effective strategy to prepare HAp/MAE‐LDH scaffolds for enhanced bone regeneration. The surface functionalization of MAE‐LDHs on the porous HAp scaffold can significantly improve its surface roughness, specific surface, and hydrophilicity, thus effectively boosting the cells adhesion and osteogenic differentiation. Importantly, the MAE‐LDHs grown on HAp scaffolds enable the sustained release of Mg2+ and Eu3+ ions for efficient bone repair and vascular regeneration. In vitro experiments suggest that the HAp/MAE‐LDH scaffold presents much enhanced osteogenesis and angiogenesis properties in comparison with the pristine HAp scaffold. In vivo assays further reveal that the new bone mass and mineral density of HAp/MAE‐LDH scaffold increased by 3.18‐ and 2.21‐fold, respectively, than that of pristine HAp scaffold. The transcriptome sequencing analysis reveals that the HAp/MAE‐LDH scaffold can activate the Wnt/β‐catenin signaling pathway to promote the osteogenic and angiogenic abilities. |
| first_indexed | 2024-04-11T01:09:15Z |
| format | Article |
| id | doaj.art-f77eb936490f45f1aba3b83aac38964d |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-11T01:09:15Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-f77eb936490f45f1aba3b83aac38964d2023-01-04T10:53:45ZengWileyAdvanced Science2198-38442023-01-01101n/an/a10.1002/advs.202204234Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone RegenerationGuanyun Wang0Zehui Lv1Tao Wang2Tingting Hu3Yixin Bian4Yu Yang5Ruizheng Liang6Chaoliang Tan7Xisheng Weng8Department of Orthopedic Surgery State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College Beijing 100730 ChinaDepartment of Orthopedic Surgery State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College Beijing 100730 ChinaState Key Laboratory of Chemical Resource Engineering Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 P. R. ChinaState Key Laboratory of Chemical Resource Engineering Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 P. R. ChinaDepartment of Orthopedic Surgery State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College Beijing 100730 ChinaState Key Laboratory of Chemical Resource Engineering Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 P. R. ChinaState Key Laboratory of Chemical Resource Engineering Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 P. R. ChinaDepartment of Chemistry and Center of Super‐Diamond and Advanced Films (COSDAF) City University of Hong Kong Kowloon Hong Kong SAR ChinaDepartment of Orthopedic Surgery State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College Beijing 100730 ChinaAbstract Although artificial bone repair scaffolds, such as titanium alloy, bioactive glass, and hydroxyapatite (HAp), have been widely used for treatment of large‐size bone defects or serious bone destruction, they normally exhibit unsatisfied bone repair efficiency because of their weak osteogenic and angiogenesis performance as well as poor cell crawling and adhesion properties. Herein, the surface functionalization of MgAlEu‐layered double hydroxide (MAE‐LDH) nanosheets on porous HAp scaffolds is reported as a simple and effective strategy to prepare HAp/MAE‐LDH scaffolds for enhanced bone regeneration. The surface functionalization of MAE‐LDHs on the porous HAp scaffold can significantly improve its surface roughness, specific surface, and hydrophilicity, thus effectively boosting the cells adhesion and osteogenic differentiation. Importantly, the MAE‐LDHs grown on HAp scaffolds enable the sustained release of Mg2+ and Eu3+ ions for efficient bone repair and vascular regeneration. In vitro experiments suggest that the HAp/MAE‐LDH scaffold presents much enhanced osteogenesis and angiogenesis properties in comparison with the pristine HAp scaffold. In vivo assays further reveal that the new bone mass and mineral density of HAp/MAE‐LDH scaffold increased by 3.18‐ and 2.21‐fold, respectively, than that of pristine HAp scaffold. The transcriptome sequencing analysis reveals that the HAp/MAE‐LDH scaffold can activate the Wnt/β‐catenin signaling pathway to promote the osteogenic and angiogenic abilities.https://doi.org/10.1002/advs.202204234bone repair and regenerationhydroxyapatite scaffoldsMgAlEu‐layered double hydroxide nanosheetssurface functionalization |
| spellingShingle | Guanyun Wang Zehui Lv Tao Wang Tingting Hu Yixin Bian Yu Yang Ruizheng Liang Chaoliang Tan Xisheng Weng Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone Regeneration Advanced Science bone repair and regeneration hydroxyapatite scaffolds MgAlEu‐layered double hydroxide nanosheets surface functionalization |
| title | Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone Regeneration |
| title_full | Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone Regeneration |
| title_fullStr | Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone Regeneration |
| title_full_unstemmed | Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone Regeneration |
| title_short | Surface Functionalization of Hydroxyapatite Scaffolds with MgAlEu‐LDH Nanosheets for High‐Performance Bone Regeneration |
| title_sort | surface functionalization of hydroxyapatite scaffolds with mgaleu ldh nanosheets for high performance bone regeneration |
| topic | bone repair and regeneration hydroxyapatite scaffolds MgAlEu‐layered double hydroxide nanosheets surface functionalization |
| url | https://doi.org/10.1002/advs.202204234 |
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