Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients
OBJECTIVE: To identify the well-known common translocations and FLT3 mutations in childhood acute myelogenous leukemia (AML) patients in Turkey. METHODS: The study included 50 newly diagnosed patients in which t(15;17), t(8;21), and inv(16) chromosomal translocations were identified using real-time...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2012-08-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-24392 |
| _version_ | 1797912472254939136 |
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| author | Ender Coşkunpınar Sema Anak Leyla Ağaoğlu Ayşegül Ünüvar Ömer Devecioğlu Gönül Aydoğan Çetin Timur Ahmet Faik Öner Yıldız Yıldırmak Tiraje Celkan İnci Yıldız Nazan Sarper Uğur Özbek |
| author_facet | Ender Coşkunpınar Sema Anak Leyla Ağaoğlu Ayşegül Ünüvar Ömer Devecioğlu Gönül Aydoğan Çetin Timur Ahmet Faik Öner Yıldız Yıldırmak Tiraje Celkan İnci Yıldız Nazan Sarper Uğur Özbek |
| author_sort | Ender Coşkunpınar |
| collection | DOAJ |
| description | OBJECTIVE: To identify the well-known common translocations and FLT3 mutations in childhood acute myelogenous leukemia (AML) patients in Turkey. METHODS: The study included 50 newly diagnosed patients in which t(15;17), t(8;21), and inv(16) chromosomal translocations were identified using real-time PCR and FLT3 gene mutations were identified via direct PCR amplification PCR-RE analysis. RESULTS: In all, t(15;17) chromosomal aberrations were observed in 4 patients (8.0%), t(8;21) chromosomal aberrations were observed in 12 patients (24.0%), inv(16) chromosomal aberrations were observed in 3 patients (6.0%), and FLT3-ITD mutations were observed in 2 patients (4.0%); FLT3-D835 point mutation heterozygosity was observed in only 1 patient (2.0%) patient. CONCLUSION: Despite of the known literature, our case shows a better survival and this might be due to the complementation effect of the t(15;17) translocation. The lower mutation rate (4%) of FLT3 gene seems to be one of the first results for Turkish population. |
| first_indexed | 2024-04-10T11:56:30Z |
| format | Article |
| id | doaj.art-f78084826cb8469e8e42f70e9db5c5c6 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T11:56:30Z |
| publishDate | 2012-08-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-f78084826cb8469e8e42f70e9db5c5c62023-02-15T16:16:47ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632012-08-0129322523210.5505/tjh.2012.24392TJH-24392Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia PatientsEnder Coşkunpınar0Sema Anak1Leyla Ağaoğlu2Ayşegül Ünüvar3Ömer Devecioğlu4Gönül Aydoğan5Çetin Timur6Ahmet Faik Öner7Yıldız Yıldırmak8Tiraje Celkan9İnci Yıldız10Nazan Sarper11Uğur Özbek12İstanbul University, Institute Of Experimental Medical Research, Department Of Genetics, İstanbul, Turkeyİstanbul University, School Of Medicine, Department Of Pediatric Hematology-oncology, İstanbul, Turkeyİstanbul University, School Of Medicine, Department Of Pediatric Hematology-oncology, İstanbul, Turkeyİstanbul University, School Of Medicine, Department Of Pediatric Hematology-oncology, İstanbul, Turkeyİstanbul University, School Of Medicine, Department Of Pediatric Hematology-oncology, İstanbul, TurkeyBakırköy Maternity And Children's Hospital, İstanbul, TurkeyGöztepe Education And Research Hospital, Department Of Pediatric Hematology, İstanbul, TurkeyYüzüncü Yıl University, School Of Medicine, Department Of Pediatrics, Van, TurkeyŞişli Etfal Education And Research Hospital, Department Of Pediatric Hematology, İstanbul, Turkeyİstanbul University, Cerrahpaşa School Of Medicine, Department Of Pediatric Hematology-oncology, İstanbul, Turkeyİstanbul University, Cerrahpaşa School Of Medicine, Department Of Pediatric Hematology-oncology, İstanbul, TurkeyKocaeli University, School Of Medicine, Department Of Children's Health And Diseases, Kocaeli, Turkeyİstanbul University, Institute Of Experimental Medical Research, Department Of Genetics, İstanbul, TurkeyOBJECTIVE: To identify the well-known common translocations and FLT3 mutations in childhood acute myelogenous leukemia (AML) patients in Turkey. METHODS: The study included 50 newly diagnosed patients in which t(15;17), t(8;21), and inv(16) chromosomal translocations were identified using real-time PCR and FLT3 gene mutations were identified via direct PCR amplification PCR-RE analysis. RESULTS: In all, t(15;17) chromosomal aberrations were observed in 4 patients (8.0%), t(8;21) chromosomal aberrations were observed in 12 patients (24.0%), inv(16) chromosomal aberrations were observed in 3 patients (6.0%), and FLT3-ITD mutations were observed in 2 patients (4.0%); FLT3-D835 point mutation heterozygosity was observed in only 1 patient (2.0%) patient. CONCLUSION: Despite of the known literature, our case shows a better survival and this might be due to the complementation effect of the t(15;17) translocation. The lower mutation rate (4%) of FLT3 gene seems to be one of the first results for Turkish population.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-24392childhood amlflt3 gene mutationsitdd835 mutationschromosomal translocations |
| spellingShingle | Ender Coşkunpınar Sema Anak Leyla Ağaoğlu Ayşegül Ünüvar Ömer Devecioğlu Gönül Aydoğan Çetin Timur Ahmet Faik Öner Yıldız Yıldırmak Tiraje Celkan İnci Yıldız Nazan Sarper Uğur Özbek Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients Turkish Journal of Hematology childhood aml flt3 gene mutations itd d835 mutations chromosomal translocations |
| title | Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients |
| title_full | Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients |
| title_fullStr | Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients |
| title_full_unstemmed | Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients |
| title_short | Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients |
| title_sort | analysis of chromosomal aberrations and flt3 gene mutations in childhood acute myelogenous leukemia patients |
| topic | childhood aml flt3 gene mutations itd d835 mutations chromosomal translocations |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-24392 |
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