Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case Study
The objective of this work was to study the efficiency of different autolysis processes, combining different temperatures and pH conditions, when applied to a genetically engineered yeast residue. The determination of the supernatants’ dry weight showed that the autolysis time could be reduced to ha...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/9/7/673 |
| _version_ | 1797589408935837696 |
|---|---|
| author | Joana F. Fundo Teresa Deuchande Daniela A. Rodrigues Lígia L. Pimentel Susana S. M. P. Vidigal Luís M. Rodríguez-Alcalá Manuela E. Pintado Ana L. Amaro |
| author_facet | Joana F. Fundo Teresa Deuchande Daniela A. Rodrigues Lígia L. Pimentel Susana S. M. P. Vidigal Luís M. Rodríguez-Alcalá Manuela E. Pintado Ana L. Amaro |
| author_sort | Joana F. Fundo |
| collection | DOAJ |
| description | The objective of this work was to study the efficiency of different autolysis processes, combining different temperatures and pH conditions, when applied to a genetically engineered yeast residue. The determination of the supernatants’ dry weight showed that the autolysis time could be reduced to half, from 4 to 2 h, if the residue pH was increased from 5 to 8 at 50 °C (18.20% for 4 h and 18.70% for 2 h with a higher pH). This result allowed us to select a short autolysis time to proceed with the second part of the experiments. The application of this faster induced autolysis process enabled us to obtain supernatants with higher concentrations of relevant compounds, such as some amino acids and minerals. An increase in leucine (of around 7%), aspartic acid, valine, phenylalanine, isoleucine and serine (approximately 2%) was observed in the autolyzed samples, when compared to the untreated ones. Also, regarding minerals, the autolysis process allowed us to obtain significantly higher amounts of potassium in the treated samples’ supernatants. This work allowed the selection of a fast and low-cost induced autolysis process for synthetic biotechnology-derived spent yeast residue to attain a product rich in high-value compounds, which can be used in commercial applications, for example, as an animal feed additive. |
| first_indexed | 2024-03-11T01:06:14Z |
| format | Article |
| id | doaj.art-f7869a29d10b4da39e9a39e2b856b3d9 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:06:14Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-f7869a29d10b4da39e9a39e2b856b3d92023-11-18T19:16:48ZengMDPI AGFermentation2311-56372023-07-019767310.3390/fermentation9070673Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case StudyJoana F. Fundo0Teresa Deuchande1Daniela A. Rodrigues2Lígia L. Pimentel3Susana S. M. P. Vidigal4Luís M. Rodríguez-Alcalá5Manuela E. Pintado6Ana L. Amaro7CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalThe objective of this work was to study the efficiency of different autolysis processes, combining different temperatures and pH conditions, when applied to a genetically engineered yeast residue. The determination of the supernatants’ dry weight showed that the autolysis time could be reduced to half, from 4 to 2 h, if the residue pH was increased from 5 to 8 at 50 °C (18.20% for 4 h and 18.70% for 2 h with a higher pH). This result allowed us to select a short autolysis time to proceed with the second part of the experiments. The application of this faster induced autolysis process enabled us to obtain supernatants with higher concentrations of relevant compounds, such as some amino acids and minerals. An increase in leucine (of around 7%), aspartic acid, valine, phenylalanine, isoleucine and serine (approximately 2%) was observed in the autolyzed samples, when compared to the untreated ones. Also, regarding minerals, the autolysis process allowed us to obtain significantly higher amounts of potassium in the treated samples’ supernatants. This work allowed the selection of a fast and low-cost induced autolysis process for synthetic biotechnology-derived spent yeast residue to attain a product rich in high-value compounds, which can be used in commercial applications, for example, as an animal feed additive.https://www.mdpi.com/2311-5637/9/7/673synthetic biotechnologyspent yeast residuenutrient sourceautolysisphysicochemical compositionanimal feed |
| spellingShingle | Joana F. Fundo Teresa Deuchande Daniela A. Rodrigues Lígia L. Pimentel Susana S. M. P. Vidigal Luís M. Rodríguez-Alcalá Manuela E. Pintado Ana L. Amaro Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case Study Fermentation synthetic biotechnology spent yeast residue nutrient source autolysis physicochemical composition animal feed |
| title | Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case Study |
| title_full | Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case Study |
| title_fullStr | Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case Study |
| title_full_unstemmed | Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case Study |
| title_short | Induced Autolysis of Engineered Yeast Residue as a Means to Simplify Downstream Processing for Valorization—A Case Study |
| title_sort | induced autolysis of engineered yeast residue as a means to simplify downstream processing for valorization a case study |
| topic | synthetic biotechnology spent yeast residue nutrient source autolysis physicochemical composition animal feed |
| url | https://www.mdpi.com/2311-5637/9/7/673 |
| work_keys_str_mv | AT joanaffundo inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy AT teresadeuchande inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy AT danielaarodrigues inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy AT ligialpimentel inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy AT susanasmpvidigal inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy AT luismrodriguezalcala inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy AT manuelaepintado inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy AT analamaro inducedautolysisofengineeredyeastresidueasameanstosimplifydownstreamprocessingforvalorizationacasestudy |