The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site
In Drosophila, Dicer-1 binds Loquacious-PB (Loqs-PB) as its major co-factor. Previous analyses indicated that loqs mutants only partially impede miRNA processing, but the activity of minor isoforms or maternally deposited Loqs was not eliminated in these studies. We addressed this by generating a ce...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-05-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124716305034 |
| _version_ | 1831692124265906176 |
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| author | Mandy Yu Theng Lim Alvin Wei Tian Ng Yuting Chou Teck Por Lim Amanda Simcox Greg Tucker-Kellogg Katsutomo Okamura |
| author_facet | Mandy Yu Theng Lim Alvin Wei Tian Ng Yuting Chou Teck Por Lim Amanda Simcox Greg Tucker-Kellogg Katsutomo Okamura |
| author_sort | Mandy Yu Theng Lim |
| collection | DOAJ |
| description | In Drosophila, Dicer-1 binds Loquacious-PB (Loqs-PB) as its major co-factor. Previous analyses indicated that loqs mutants only partially impede miRNA processing, but the activity of minor isoforms or maternally deposited Loqs was not eliminated in these studies. We addressed this by generating a cell line from loqs-null embryos and found that only ∼40% of miRNAs showed clear Loqs dependence. Genome-wide comparison of the hairpin structure and Loqs dependence suggested that Loqs substrates are influenced by base-pairing status at the dicing site. Artificial alteration of base-pairing stability at this position in model miRNA hairpins resulted in predicted changes in Loqs dependence, providing evidence for this hypothesis. Finally, we found that evolutionarily young miRNA genes tended to be Loqs dependent. We propose that Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates. |
| first_indexed | 2024-12-20T11:44:06Z |
| format | Article |
| id | doaj.art-f786c98e1e3e42dfb1f3129a3f70a93e |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-20T11:44:06Z |
| publishDate | 2016-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-f786c98e1e3e42dfb1f3129a3f70a93e2022-12-21T19:41:55ZengElsevierCell Reports2211-12472016-05-011581795180810.1016/j.celrep.2016.04.059The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing SiteMandy Yu Theng Lim0Alvin Wei Tian Ng1Yuting Chou2Teck Por Lim3Amanda Simcox4Greg Tucker-Kellogg5Katsutomo Okamura6Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, SingaporeDepartment of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, SingaporeSloan-Kettering Institute, Department of Developmental Biology, New York, NY 10065, USADepartment of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, SingaporeDepartment of Molecular Genetics, Ohio State University, Columbus, OH 43210, USADepartment of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, SingaporeTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, SingaporeIn Drosophila, Dicer-1 binds Loquacious-PB (Loqs-PB) as its major co-factor. Previous analyses indicated that loqs mutants only partially impede miRNA processing, but the activity of minor isoforms or maternally deposited Loqs was not eliminated in these studies. We addressed this by generating a cell line from loqs-null embryos and found that only ∼40% of miRNAs showed clear Loqs dependence. Genome-wide comparison of the hairpin structure and Loqs dependence suggested that Loqs substrates are influenced by base-pairing status at the dicing site. Artificial alteration of base-pairing stability at this position in model miRNA hairpins resulted in predicted changes in Loqs dependence, providing evidence for this hypothesis. Finally, we found that evolutionarily young miRNA genes tended to be Loqs dependent. We propose that Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates.http://www.sciencedirect.com/science/article/pii/S2211124716305034 |
| spellingShingle | Mandy Yu Theng Lim Alvin Wei Tian Ng Yuting Chou Teck Por Lim Amanda Simcox Greg Tucker-Kellogg Katsutomo Okamura The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site Cell Reports |
| title | The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site |
| title_full | The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site |
| title_fullStr | The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site |
| title_full_unstemmed | The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site |
| title_short | The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site |
| title_sort | drosophila dicer 1 partner loquacious enhances mirna processing from hairpins with unstable structures at the dicing site |
| url | http://www.sciencedirect.com/science/article/pii/S2211124716305034 |
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