Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i>
Zincoxide nanoparticles (ZnO NPs) are among the most produced and used nanomaterials worldwide, and in recent times these nanoparticles have also been incorporate in plant science and agricultural research. The present study was planned to synthesize ZnO NPs biologically using <i>Ochradenus ar...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/9/1808 |
| _version_ | 1797517535656017920 |
|---|---|
| author | Hassan O. Shaikhaldein Fahad Al-Qurainy Salim Khan Mohammad Nadeem Mohamed Tarroum Abdalrhaman M. Salih Abdel-Rhman Zakaria Gaafar Aref Alshameri Saleh Alansi Norah Arrak Alenezi Norah S. Alfarraj |
| author_facet | Hassan O. Shaikhaldein Fahad Al-Qurainy Salim Khan Mohammad Nadeem Mohamed Tarroum Abdalrhaman M. Salih Abdel-Rhman Zakaria Gaafar Aref Alshameri Saleh Alansi Norah Arrak Alenezi Norah S. Alfarraj |
| author_sort | Hassan O. Shaikhaldein |
| collection | DOAJ |
| description | Zincoxide nanoparticles (ZnO NPs) are among the most produced and used nanomaterials worldwide, and in recent times these nanoparticles have also been incorporate in plant science and agricultural research. The present study was planned to synthesize ZnO NPs biologically using <i>Ochradenus arabicus</i> leaves and examine their effect on the morphology and physiology properties of <i>Maerua oblongifolia</i> cultured in vitro. ZnO NPs were characterized by UV–visible spectroscopy (UV–vis), X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy, which demonstrated hexagonal shape nanoparticles of size ranging from 10 to 50 nm. Thus, the study uncovered an efficient, eco-friendly and simple technique for biosynthesis of multifunctional ZnO NPs using <i>Ochradenus arabicus</i> following growth of <i>Maerua oblongifolia</i> shoots in different concentrations of ZnO NPs (0, 1.25, 2.5, 5, 10, or 20 mg L<sup>−1</sup>) in Murashige and Skoog medium. Remarkable increases in plant biomass, photosynthetic pigments, and total protein were recorded up to a concentration of 5 mg L<sup>−1</sup>; at the same time, the results demonstrated a significant reduction in lipid peroxidation levels with respect to control. Interestingly, the levels of proline and the antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR) activities were increased significantly in response to all ZnO NP treatments. These findings indicate that bioengineered ZnO NPs play a major role in accumulation of biomass and stimulating the activities of antioxidant enzymes in plant tissues. Thus, green-synthesized ZnO NPs might be of agricultural and medicinal benefit owing to their impacts on plants in vitro. |
| first_indexed | 2024-03-10T07:17:54Z |
| format | Article |
| id | doaj.art-f78929ef45bc40299bd710b0b78e1442 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:54Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-f78929ef45bc40299bd710b0b78e14422023-11-22T14:52:01ZengMDPI AGPlants2223-77472021-08-01109180810.3390/plants10091808Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i>Hassan O. Shaikhaldein0Fahad Al-Qurainy1Salim Khan2Mohammad Nadeem3Mohamed Tarroum4Abdalrhaman M. Salih5Abdel-Rhman Zakaria Gaafar6Aref Alshameri7Saleh Alansi8Norah Arrak Alenezi9Norah S. Alfarraj10Department of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaZincoxide nanoparticles (ZnO NPs) are among the most produced and used nanomaterials worldwide, and in recent times these nanoparticles have also been incorporate in plant science and agricultural research. The present study was planned to synthesize ZnO NPs biologically using <i>Ochradenus arabicus</i> leaves and examine their effect on the morphology and physiology properties of <i>Maerua oblongifolia</i> cultured in vitro. ZnO NPs were characterized by UV–visible spectroscopy (UV–vis), X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy, which demonstrated hexagonal shape nanoparticles of size ranging from 10 to 50 nm. Thus, the study uncovered an efficient, eco-friendly and simple technique for biosynthesis of multifunctional ZnO NPs using <i>Ochradenus arabicus</i> following growth of <i>Maerua oblongifolia</i> shoots in different concentrations of ZnO NPs (0, 1.25, 2.5, 5, 10, or 20 mg L<sup>−1</sup>) in Murashige and Skoog medium. Remarkable increases in plant biomass, photosynthetic pigments, and total protein were recorded up to a concentration of 5 mg L<sup>−1</sup>; at the same time, the results demonstrated a significant reduction in lipid peroxidation levels with respect to control. Interestingly, the levels of proline and the antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR) activities were increased significantly in response to all ZnO NP treatments. These findings indicate that bioengineered ZnO NPs play a major role in accumulation of biomass and stimulating the activities of antioxidant enzymes in plant tissues. Thus, green-synthesized ZnO NPs might be of agricultural and medicinal benefit owing to their impacts on plants in vitro.https://www.mdpi.com/2223-7747/10/9/1808ZnO NPsgreen-synthesize<i>M. oblongifolia</i>chlorophyllprolineantioxidant enzymes |
| spellingShingle | Hassan O. Shaikhaldein Fahad Al-Qurainy Salim Khan Mohammad Nadeem Mohamed Tarroum Abdalrhaman M. Salih Abdel-Rhman Zakaria Gaafar Aref Alshameri Saleh Alansi Norah Arrak Alenezi Norah S. Alfarraj Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i> Plants ZnO NPs green-synthesize <i>M. oblongifolia</i> chlorophyll proline antioxidant enzymes |
| title | Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i> |
| title_full | Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i> |
| title_fullStr | Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i> |
| title_full_unstemmed | Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i> |
| title_short | Biosynthesis and Characterization of ZnO Nanoparticles Using <i>Ochradenus arabicus</i> and Their Effect on Growth and Antioxidant Systems of <i>Maerua oblongifolia</i> |
| title_sort | biosynthesis and characterization of zno nanoparticles using i ochradenus arabicus i and their effect on growth and antioxidant systems of i maerua oblongifolia i |
| topic | ZnO NPs green-synthesize <i>M. oblongifolia</i> chlorophyll proline antioxidant enzymes |
| url | https://www.mdpi.com/2223-7747/10/9/1808 |
| work_keys_str_mv | AT hassanoshaikhaldein biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT fahadalqurainy biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT salimkhan biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT mohammadnadeem biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT mohamedtarroum biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT abdalrhamanmsalih biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT abdelrhmanzakariagaafar biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT arefalshameri biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT salehalansi biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT noraharrakalenezi biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai AT norahsalfarraj biosynthesisandcharacterizationofznonanoparticlesusingiochradenusarabicusiandtheireffectongrowthandantioxidantsystemsofimaeruaoblongifoliai |