Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection.
In this study we have reported the in vivo proteomic changes during Japanese Encephalitis Virus (JEV) infection in combination with in vitro studies which will help in the comprehensive characterization of the modifications in the host metabolism in response to JEV infection. We performed a 2-DE bas...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3943924?pdf=render |
| _version_ | 1818577984663060480 |
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| author | Nabonita Sengupta Sourish Ghosh Suhas V Vasaikar James Gomes Anirban Basu |
| author_facet | Nabonita Sengupta Sourish Ghosh Suhas V Vasaikar James Gomes Anirban Basu |
| author_sort | Nabonita Sengupta |
| collection | DOAJ |
| description | In this study we have reported the in vivo proteomic changes during Japanese Encephalitis Virus (JEV) infection in combination with in vitro studies which will help in the comprehensive characterization of the modifications in the host metabolism in response to JEV infection. We performed a 2-DE based quantitative proteomic study of JEV-infected mouse brain as well as mouse neuroblastoma (Neuro2a) cells to analyze the host response to this lethal virus. 56 host proteins were found to be differentially expressed post JEV infection (defined as exhibiting ≥ 1.5-fold change in protein abundance upon JEV infection). Bioinformatics analyses were used to generate JEV-regulated host response networks which reported that the identified proteins were found to be associated with various cellular processes ranging from intracellular protein transport, cellular metabolism and ER stress associated unfolded protein response. JEV was found to invade the host protein folding machinery to sustain its survival and replication inside the host thereby generating a vigorous unfolded protein response, subsequently triggering a number of pathways responsible for the JEV associated pathologies. The results were also validated using a human cell line to correlate them to the human response to JEV. The present investigation is the first report on JEV-host interactome in in vivo model and will be of potential interest for future antiviral research in this field. |
| first_indexed | 2024-12-16T06:38:36Z |
| format | Article |
| id | doaj.art-f789506c83934d54973974f3267256c6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T06:38:36Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f789506c83934d54973974f3267256c62022-12-21T22:40:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9021110.1371/journal.pone.0090211Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection.Nabonita SenguptaSourish GhoshSuhas V VasaikarJames GomesAnirban BasuIn this study we have reported the in vivo proteomic changes during Japanese Encephalitis Virus (JEV) infection in combination with in vitro studies which will help in the comprehensive characterization of the modifications in the host metabolism in response to JEV infection. We performed a 2-DE based quantitative proteomic study of JEV-infected mouse brain as well as mouse neuroblastoma (Neuro2a) cells to analyze the host response to this lethal virus. 56 host proteins were found to be differentially expressed post JEV infection (defined as exhibiting ≥ 1.5-fold change in protein abundance upon JEV infection). Bioinformatics analyses were used to generate JEV-regulated host response networks which reported that the identified proteins were found to be associated with various cellular processes ranging from intracellular protein transport, cellular metabolism and ER stress associated unfolded protein response. JEV was found to invade the host protein folding machinery to sustain its survival and replication inside the host thereby generating a vigorous unfolded protein response, subsequently triggering a number of pathways responsible for the JEV associated pathologies. The results were also validated using a human cell line to correlate them to the human response to JEV. The present investigation is the first report on JEV-host interactome in in vivo model and will be of potential interest for future antiviral research in this field.http://europepmc.org/articles/PMC3943924?pdf=render |
| spellingShingle | Nabonita Sengupta Sourish Ghosh Suhas V Vasaikar James Gomes Anirban Basu Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection. PLoS ONE |
| title | Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection. |
| title_full | Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection. |
| title_fullStr | Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection. |
| title_full_unstemmed | Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection. |
| title_short | Modulation of neuronal proteome profile in response to Japanese encephalitis virus infection. |
| title_sort | modulation of neuronal proteome profile in response to japanese encephalitis virus infection |
| url | http://europepmc.org/articles/PMC3943924?pdf=render |
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