Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine
Interleukin-6 (IL-6) could be decomposed by irradiation of IR-FEL (Infrared free electron laser). Using circularly polarized and other UV light and IR-FEL light, photolysis of hierarchical components of cast films of IL-6, namely deuterated aqueous solutions of enantiomers of dipeptide (L-alanyl-L-a...
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Format: | Article |
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Shahid Beheshti University of Medical Sciences
2021-01-01
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Series: | Trends in Peptide and Protein Sciences |
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Online Access: | https://journals.sbmu.ac.ir/protein/article/view/33129 |
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author | Nanami Fujisawa Yuika Onami Takayasu Kawasaki Tomoyuki Haraguchi Takashiro Akitsu Koichi Tsukiyama |
author_facet | Nanami Fujisawa Yuika Onami Takayasu Kawasaki Tomoyuki Haraguchi Takashiro Akitsu Koichi Tsukiyama |
author_sort | Nanami Fujisawa |
collection | DOAJ |
description | Interleukin-6 (IL-6) could be decomposed by irradiation of IR-FEL (Infrared free electron laser). Using circularly polarized and other UV light and IR-FEL light, photolysis of hierarchical components of cast films of IL-6, namely deuterated aqueous solutions of enantiomers of dipeptide (L-alanyl-L-alanine (Ala-ala) or D-alanyl-D-alanine) and enantiomers of amino acid (L-alanine (Ala) or D-alanine) was investigated whether specific bonds can be broken by absorption of light (not due to heat). In addition, IR-FEL irradiation to powder as well as crystal structure determination for L-Ala and D-Ala at 173 and 293 K were also carried out to confirm reproducibility in the solid state about long-lasting controversy about Salam’ hypothesis associated with chirality exhibiting structural phase transition at different temperature. Subunits of IL-6 (dipeptide and amino acid) could not be decomposed by polarized IR-FEL nor UV (ultraviolet) light regardless of their chirality. All experimental methods tested in this study failed to prove Salam's hypothesis, positively. Consequently, secondary structure of IL-6 was found to be easier to be damaged by IR-FEL than covalent bonds.
HIGHLIGHTS
• Interleukin 6 (IL-6) was decomposed by irradiation of IR-FEL (Infrared free electron laser).
• Parts of IL-6 (dipeptide and amino acid) was not decomposed by polarized IR-FEL nor UV (ultraviolet) light regardless of their chirality.
• Secondary structure of IL-6 was easier to be damaged by IR-FEL than covalent bonds. |
first_indexed | 2024-12-24T03:29:01Z |
format | Article |
id | doaj.art-f7898cc6871a4f66b03a3784898f3a94 |
institution | Directory Open Access Journal |
issn | 2538-2446 |
language | English |
last_indexed | 2024-12-24T03:29:01Z |
publishDate | 2021-01-01 |
publisher | Shahid Beheshti University of Medical Sciences |
record_format | Article |
series | Trends in Peptide and Protein Sciences |
spelling | doaj.art-f7898cc6871a4f66b03a3784898f3a942022-12-21T17:17:16ZengShahid Beheshti University of Medical SciencesTrends in Peptide and Protein Sciences2538-24462021-01-015111 (e8)10.22037/tpps.v5i.3312933129Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and AlanineNanami FujisawaYuika OnamiTakayasu KawasakiTomoyuki HaraguchiTakashiro AkitsuKoichi TsukiyamaInterleukin-6 (IL-6) could be decomposed by irradiation of IR-FEL (Infrared free electron laser). Using circularly polarized and other UV light and IR-FEL light, photolysis of hierarchical components of cast films of IL-6, namely deuterated aqueous solutions of enantiomers of dipeptide (L-alanyl-L-alanine (Ala-ala) or D-alanyl-D-alanine) and enantiomers of amino acid (L-alanine (Ala) or D-alanine) was investigated whether specific bonds can be broken by absorption of light (not due to heat). In addition, IR-FEL irradiation to powder as well as crystal structure determination for L-Ala and D-Ala at 173 and 293 K were also carried out to confirm reproducibility in the solid state about long-lasting controversy about Salam’ hypothesis associated with chirality exhibiting structural phase transition at different temperature. Subunits of IL-6 (dipeptide and amino acid) could not be decomposed by polarized IR-FEL nor UV (ultraviolet) light regardless of their chirality. All experimental methods tested in this study failed to prove Salam's hypothesis, positively. Consequently, secondary structure of IL-6 was found to be easier to be damaged by IR-FEL than covalent bonds. HIGHLIGHTS • Interleukin 6 (IL-6) was decomposed by irradiation of IR-FEL (Infrared free electron laser). • Parts of IL-6 (dipeptide and amino acid) was not decomposed by polarized IR-FEL nor UV (ultraviolet) light regardless of their chirality. • Secondary structure of IL-6 was easier to be damaged by IR-FEL than covalent bonds.https://journals.sbmu.ac.ir/protein/article/view/33129interleukin 6alanyl-alaninealanineir-felpolarized lightsalam hypothesis |
spellingShingle | Nanami Fujisawa Yuika Onami Takayasu Kawasaki Tomoyuki Haraguchi Takashiro Akitsu Koichi Tsukiyama Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine Trends in Peptide and Protein Sciences interleukin 6 alanyl-alanine alanine ir-fel polarized light salam hypothesis |
title | Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine |
title_full | Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine |
title_fullStr | Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine |
title_full_unstemmed | Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine |
title_short | Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine |
title_sort | infrared free electron laser or polarized ultraviolet photolysis of hierarchical and chiral components of interleukin 6 alanylalanine and alanine |
topic | interleukin 6 alanyl-alanine alanine ir-fel polarized light salam hypothesis |
url | https://journals.sbmu.ac.ir/protein/article/view/33129 |
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