Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing
Biomaterials containing citric acid as a building unit show potential for use as blood vessel and skin tissue substitutes. The success in commercializing implants containing a polymer matrix of poly(1,8-octanediol citrate) provides a rationale for exploring polycitrates based on other diols. Changin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1332290/full |
| _version_ | 1797261814024634368 |
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| author | Aleksandra Bandzerewicz Joanna Howis Kamil Wierzchowski Miroslav Slouf Jiri Hodan Piotr Denis Tomasz Gołofit Maciej Pilarek Agnieszka Gadomska-Gajadhur |
| author_facet | Aleksandra Bandzerewicz Joanna Howis Kamil Wierzchowski Miroslav Slouf Jiri Hodan Piotr Denis Tomasz Gołofit Maciej Pilarek Agnieszka Gadomska-Gajadhur |
| author_sort | Aleksandra Bandzerewicz |
| collection | DOAJ |
| description | Biomaterials containing citric acid as a building unit show potential for use as blood vessel and skin tissue substitutes. The success in commercializing implants containing a polymer matrix of poly(1,8-octanediol citrate) provides a rationale for exploring polycitrates based on other diols. Changing the aliphatic chain length of the diol allows functional design strategies to control the implant’s mechanical properties, degradation profile and surface energy. In the present work, poly(1,2-ethanediol citrate) was synthesized and used as an additive to polylactide in the electrospinning process. It was established that the content of polycitrate greatly influences the nonwovens’ properties: an equal mass ratio of polymers resulted in the best morphology. The obtained nonwovens were characterized by surface hydrophilicity, tensile strength, and thermal properties. L929 cell cultures were carried out on their surface. The materials were found to be non-cytotoxic and the degree of porosity was suitable for cell colonization. On the basis of the most important parameters for assessing the condition of cultured cells (cell density and viability, cell metabolic activity and lactate dehydrogenase activity), the potential of PLLA + PECit nonwovens for application in tissue engineering was established. |
| first_indexed | 2024-04-24T23:47:11Z |
| format | Article |
| id | doaj.art-f791fbdb8eff41268fe08212f938e296 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-24T23:47:11Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-f791fbdb8eff41268fe08212f938e2962024-03-15T04:52:55ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-03-011210.3389/fbioe.2024.13322901332290Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturingAleksandra Bandzerewicz0Joanna Howis1Kamil Wierzchowski2Miroslav Slouf3Jiri Hodan4Piotr Denis5Tomasz Gołofit6Maciej Pilarek7Agnieszka Gadomska-Gajadhur8Faculty of Chemistry, Warsaw University of Technology, Warsaw, PolandFaculty of Chemistry, Warsaw University of Technology, Warsaw, PolandFaculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, PolandInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Prague, CzechiaLaboratory of Polymers and Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, PolandFaculty of Chemistry, Warsaw University of Technology, Warsaw, PolandFaculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, PolandFaculty of Chemistry, Warsaw University of Technology, Warsaw, PolandBiomaterials containing citric acid as a building unit show potential for use as blood vessel and skin tissue substitutes. The success in commercializing implants containing a polymer matrix of poly(1,8-octanediol citrate) provides a rationale for exploring polycitrates based on other diols. Changing the aliphatic chain length of the diol allows functional design strategies to control the implant’s mechanical properties, degradation profile and surface energy. In the present work, poly(1,2-ethanediol citrate) was synthesized and used as an additive to polylactide in the electrospinning process. It was established that the content of polycitrate greatly influences the nonwovens’ properties: an equal mass ratio of polymers resulted in the best morphology. The obtained nonwovens were characterized by surface hydrophilicity, tensile strength, and thermal properties. L929 cell cultures were carried out on their surface. The materials were found to be non-cytotoxic and the degree of porosity was suitable for cell colonization. On the basis of the most important parameters for assessing the condition of cultured cells (cell density and viability, cell metabolic activity and lactate dehydrogenase activity), the potential of PLLA + PECit nonwovens for application in tissue engineering was established.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1332290/fullcitric acidcitrate-based polyestersbiomaterialsnonwovenselectrospinning |
| spellingShingle | Aleksandra Bandzerewicz Joanna Howis Kamil Wierzchowski Miroslav Slouf Jiri Hodan Piotr Denis Tomasz Gołofit Maciej Pilarek Agnieszka Gadomska-Gajadhur Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing Frontiers in Bioengineering and Biotechnology citric acid citrate-based polyesters biomaterials nonwovens electrospinning |
| title | Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing |
| title_full | Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing |
| title_fullStr | Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing |
| title_full_unstemmed | Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing |
| title_short | Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing |
| title_sort | exploring the application of poly 1 2 ethanediol citrate polylactide nonwovens in cell culturing |
| topic | citric acid citrate-based polyesters biomaterials nonwovens electrospinning |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1332290/full |
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