XRCC-1 Gene Polymorphism (Arg399Gln) and Susceptibility to Development of Lung Cancer in Cohort of North Indian Population: A Pilot Study
Background: Smoking has been considered to be the major cause of lung cancer. However, only a fraction of cigarette smokers develop this disease. This suggests the importance of genetic constitution in predicting the individual’s susceptibility towards lung cancer. This genetic susceptibility ma...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2014-11-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5132/10061_CE(NJ)_F(Sh)_PF1(NJAK)_PFA(Sh).pdf |
| Summary: | Background: Smoking has been considered to be the major
cause of lung cancer. However, only a fraction of cigarette
smokers develop this disease. This suggests the importance of
genetic constitution in predicting the individual’s susceptibility
towards lung cancer. This genetic susceptibility may result
from inherited polymorphisms in genes controlling carcinogen
metabolism and repair of damaged deoxyribonucleic acid (DNA).
These repair systems are fundamental to the maintenance of
genomic integrity. X-ray repair cross complimenting group I
(XRCC1), a major DNA repair gene in the base excision repair
(BER) pathway. It is involved in repair by interacting with
components of DNA at the site of damage. Inconsistent results
have been reported regarding the associations between the
Arg399Gln polymorphism of XRCC1.
This study demonstrates the importance of recognition of this
relationship of lung carcinoma and genetic constitution of the
person which will help guide clinicians on the optimal screening
of this disease.
Aim: To assess the role of XRCC1 gene polymorphism
(Arg399Gln) directly on the variation in susceptibility to
development of lung cancer in North Indian subjects.
Materials and Methods: One hundred males with diagnosed
cases of lung cancer were recruited from Delhi State Cancer
Institute (DSCI). Hundred healthy volunteers were taken as
controls. DNA isolation was done and Polymerase chain
reaction–Restriction Fragment Length Polymorphism (PCR–
RFLP) procedure undertaken to amplify the region containing
Arg/Gln substitution at codon 399 (in exon 10).
Results: XRCC1 gene polymorphism is associated with
increased risk of lung cancer when the Arg/Arg genotype was
used as the reference group. The Arg/Gln and Gln/Gln was
associated with statistically increased risk for cancer.
Conclusion: Arg399Gln polymorphism in XRCC1 gene
polymorphism is associated with lung cancer in North Indian
subjects and screening for this polymorphism will help in
targeting predisposed individuals and its prevention. |
|---|---|
| ISSN: | 2249-782X 0973-709X |