Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm Larvae
The western corn rootworm (WCR), <i>Diabrotica virgifera</i> LeConte, is the most serious pest of maize in the United States. In pursuit of developing a diet free of antibiotics for WCR, we characterized effects of thermal exposure (50–141 °C) and length of exposure on quality of WCRMO-2...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/9/783 |
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| author | Man P. Huynh Adriano E. Pereira Ryan W. Geisert Michael G. Vella Thomas A. Coudron Kent S. Shelby Bruce E. Hibbard |
| author_facet | Man P. Huynh Adriano E. Pereira Ryan W. Geisert Michael G. Vella Thomas A. Coudron Kent S. Shelby Bruce E. Hibbard |
| author_sort | Man P. Huynh |
| collection | DOAJ |
| description | The western corn rootworm (WCR), <i>Diabrotica virgifera</i> LeConte, is the most serious pest of maize in the United States. In pursuit of developing a diet free of antibiotics for WCR, we characterized effects of thermal exposure (50–141 °C) and length of exposure on quality of WCRMO-2 diet measured by life history parameters of larvae (weight, molting, and survival) reared on WCRMO-2 diet. Our results indicated that temperatures had non-linear effects on performance of WCRMO-2 diet, and no impacts were observed on the length of time exposure. The optimum temperature of diet processing was 60 °C for a duration less than 30 min. A significant decline in development was observed in larvae reared on WCRMO-2 diet pretreated above 75 °C. Exposing WCRMO-2 diet to high temperatures (110–141 °C) even if constrained for brief duration (0.9–2.3 s) caused 2-fold reduction in larval weight and significant delays in larval molting but no difference in survival for 10 days compared with the control diet prepared at 65 °C for 10 min. These findings provide insights into the effects of thermal exposure in insect diet processing. |
| first_indexed | 2024-03-10T07:34:23Z |
| format | Article |
| id | doaj.art-f793121269e64f0886cfc42e003ae0de |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T07:34:23Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-f793121269e64f0886cfc42e003ae0de2023-11-22T13:36:27ZengMDPI AGInsects2075-44502021-09-0112978310.3390/insects12090783Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm LarvaeMan P. Huynh0Adriano E. Pereira1Ryan W. Geisert2Michael G. Vella3Thomas A. Coudron4Kent S. Shelby5Bruce E. Hibbard6Division of Plant Science & Technology, University of Missouri, Columbia, MO 65211, USADivision of Plant Science & Technology, University of Missouri, Columbia, MO 65211, USADivision of Plant Science & Technology, University of Missouri, Columbia, MO 65211, USAFrontier Scientific Services, Newark, DE 19711, USABiological Control of Insects Research Laboratory, USDA-Agricultural Research Service, Columbia, MO 65203, USABiological Control of Insects Research Laboratory, USDA-Agricultural Research Service, Columbia, MO 65203, USAPlant Genetics Research Unit, USDA-Agricultural Research Service, Columbia, MO 65211, USAThe western corn rootworm (WCR), <i>Diabrotica virgifera</i> LeConte, is the most serious pest of maize in the United States. In pursuit of developing a diet free of antibiotics for WCR, we characterized effects of thermal exposure (50–141 °C) and length of exposure on quality of WCRMO-2 diet measured by life history parameters of larvae (weight, molting, and survival) reared on WCRMO-2 diet. Our results indicated that temperatures had non-linear effects on performance of WCRMO-2 diet, and no impacts were observed on the length of time exposure. The optimum temperature of diet processing was 60 °C for a duration less than 30 min. A significant decline in development was observed in larvae reared on WCRMO-2 diet pretreated above 75 °C. Exposing WCRMO-2 diet to high temperatures (110–141 °C) even if constrained for brief duration (0.9–2.3 s) caused 2-fold reduction in larval weight and significant delays in larval molting but no difference in survival for 10 days compared with the control diet prepared at 65 °C for 10 min. These findings provide insights into the effects of thermal exposure in insect diet processing.https://www.mdpi.com/2075-4450/12/9/783<i>Diabrotica virgifera</i>corn rootwormWCRMO-2diet processingheating |
| spellingShingle | Man P. Huynh Adriano E. Pereira Ryan W. Geisert Michael G. Vella Thomas A. Coudron Kent S. Shelby Bruce E. Hibbard Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm Larvae Insects <i>Diabrotica virgifera</i> corn rootworm WCRMO-2 diet processing heating |
| title | Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm Larvae |
| title_full | Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm Larvae |
| title_fullStr | Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm Larvae |
| title_full_unstemmed | Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm Larvae |
| title_short | Characterization of Thermal and Time Exposure to Improve Artificial Diet for Western Corn Rootworm Larvae |
| title_sort | characterization of thermal and time exposure to improve artificial diet for western corn rootworm larvae |
| topic | <i>Diabrotica virgifera</i> corn rootworm WCRMO-2 diet processing heating |
| url | https://www.mdpi.com/2075-4450/12/9/783 |
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