The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus
Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. All the examined farms were vaccinated using the commercial live IB-H120 vaccine in addition to the IB-inactivated vaccine in the layer farm. The...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Beni-Suef University
2010-12-01
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| Series: | Journal of Veterinary Medical Research |
| Subjects: | |
| Online Access: | https://jvmr.journals.ekb.eg/article_77637.html |
| _version_ | 1818887066553942016 |
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| author | K. M. Kamel A. Khafagy A. A. Bassiouni M. A. Afify N. S. Rabie |
| author_facet | K. M. Kamel A. Khafagy A. A. Bassiouni M. A. Afify N. S. Rabie |
| author_sort | K. M. Kamel |
| collection | DOAJ |
| description | Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. All the examined farms were vaccinated using the commercial live IB-H120 vaccine in addition to the IB-inactivated vaccine in the layer farm. The viruses were isolated and identified previously by chicken embryo, CEK cell culture inoculation. Isolates subjected to RT-PCR. Four isolates; three broiler farms and one from layer farm were genotyped using S1 partial gene sequencing. Typing of the four isolates using S1 partial gene sequencing, revealed that the isolated IBV strains showed homology to Asia, Europe, USA and Middle East strains. |
| first_indexed | 2024-12-19T16:31:19Z |
| format | Article |
| id | doaj.art-f79418bf53554cb9bd2a067f2b3cd4c9 |
| institution | Directory Open Access Journal |
| issn | 2357-0512 2357-0520 |
| language | English |
| last_indexed | 2024-12-19T16:31:19Z |
| publishDate | 2010-12-01 |
| publisher | Faculty of Veterinary Medicine, Beni-Suef University |
| record_format | Article |
| series | Journal of Veterinary Medical Research |
| spelling | doaj.art-f79418bf53554cb9bd2a067f2b3cd4c92022-12-21T20:14:10ZengFaculty of Veterinary Medicine, Beni-Suef UniversityJournal of Veterinary Medical Research2357-05122357-05202010-12-01201342350The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virusK. M. Kamel0 A. Khafagy1 A. A. Bassiouni2 M. A. Afify3N. S. Rabie4Department of Poultry Diseases, National Research Center, CairoDepartment of Poultry Diseases, Animal Health Research Institute, Dokki, GizaDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. All the examined farms were vaccinated using the commercial live IB-H120 vaccine in addition to the IB-inactivated vaccine in the layer farm. The viruses were isolated and identified previously by chicken embryo, CEK cell culture inoculation. Isolates subjected to RT-PCR. Four isolates; three broiler farms and one from layer farm were genotyped using S1 partial gene sequencing. Typing of the four isolates using S1 partial gene sequencing, revealed that the isolated IBV strains showed homology to Asia, Europe, USA and Middle East strains.https://jvmr.journals.ekb.eg/article_77637.htmlprevalence; infectious; bronchitis; ib; outbreaks; chicken; farms; molecular; characterization; field; isolates; virus |
| spellingShingle | K. M. Kamel A. Khafagy A. A. Bassiouni M. A. Afify N. S. Rabie The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus Journal of Veterinary Medical Research prevalence; infectious; bronchitis; ib; outbreaks; chicken; farms; molecular; characterization; field; isolates; virus |
| title | The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus |
| title_full | The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus |
| title_fullStr | The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus |
| title_full_unstemmed | The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus |
| title_short | The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus |
| title_sort | prevalence of infectious bronchitis ib outbreaks in some chicken farms ii molecular characterization of field isolates of ib virus |
| topic | prevalence; infectious; bronchitis; ib; outbreaks; chicken; farms; molecular; characterization; field; isolates; virus |
| url | https://jvmr.journals.ekb.eg/article_77637.html |
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