Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil
Background: It is believed that Human Papillomavirus (HPV) and Human Immunodeficiency Virus coinfection contributes to increase the risk for cervical intraepithelial injuries. Several factors may contribute to cervical cancer (CC) development, including genetic variants such as TP53 and MDM2 gene po...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867014001706 |
| _version_ | 1818500279570530304 |
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| author | Ludmila Gonçalves Entiauspe Fabiana Kömmling Seixas Emily Montosa Nunes Fernanda Martins Rodrigues Odir A. Dellagostin Tiago Collares Mariângela Freitas da Silveira |
| author_facet | Ludmila Gonçalves Entiauspe Fabiana Kömmling Seixas Emily Montosa Nunes Fernanda Martins Rodrigues Odir A. Dellagostin Tiago Collares Mariângela Freitas da Silveira |
| author_sort | Ludmila Gonçalves Entiauspe |
| collection | DOAJ |
| description | Background: It is believed that Human Papillomavirus (HPV) and Human Immunodeficiency Virus coinfection contributes to increase the risk for cervical intraepithelial injuries. Several factors may contribute to cervical cancer (CC) development, including genetic variants such as TP53 and MDM2 gene polymorphisms. Materials and methods: A hundred HIV-infected women were examined for HPV detection and its genotypes, as well as the frequencies of the SNPs Arg72Pro and SNP309 and their associations with CC risk factors. Nested Polymerase Chain Reaction (nPCR) was used for HPV detection and PCR-RFLP for TP53 and MDM2 SNP309 genotyping. Results: HPV DNA was detected in 68% of samples. A higher frequency of low-risk HPV genotypes (66.7%) was observed when compared to high-risk genotypes (33.3%). Nine different HPV genotypes were identified, with the highest prevalence of HPV-6, followed by HPV-16 and 31. p53 Arg72Arg and SNP309 TG genotype were the most prevalent. HPV genotyping was performed by sequencing. Conclusion: The data obtained suggest that HIV-infected women are more susceptible to be infected by low-risk HPV (LR-HPV) genotypes than by high-risk (HR-HPV), and Pro72Pro of TP53 gene and TG of MDM2 SNP309 genotypes apparently seem to be protective factors among HIV-infected women for HPV acquisition and HR-HPV infection, respectively, in a sample of Southern Brazilian woman. Future investigations in larger populations are necessary to better understand the potential roles of these SNPs and the behavior of non-oncogenic HPV genotypes in HIV-mediated immunosuppression cases. Keywords: HPV infection, HIV infection, Cervical cancer, Epidemiology |
| first_indexed | 2024-12-10T20:40:35Z |
| format | Article |
| id | doaj.art-f794b5bf80ad46e2bea9be119b202ecb |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-10T20:40:35Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-f794b5bf80ad46e2bea9be119b202ecb2022-12-22T01:34:23ZengElsevierBrazilian Journal of Infectious Diseases1413-86702014-11-01186643650S1413-86702014000600643Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern BrazilLudmila Gonçalves Entiauspe0Fabiana Kömmling Seixas1Emily Montosa Nunes2Fernanda Martins Rodrigues3Odir A. Dellagostin4Tiago Collares5Mariângela Freitas da Silveira6Molecular and Cellular Oncology Research Group, Biotechnology Unit, Technology Development Center, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; Post-Graduate Programme in Epidemiology, Universidade Federal de Pelotas (UFPel), Pelotas, RS, BrazilMolecular and Cellular Oncology Research Group, Biotechnology Unit, Technology Development Center, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; Post-Graduate Programme in Biotechnology, CDTec, Universidade Federal de Pelotas (UFPel), Pelotas, RS, BrazilMolecular and Cellular Oncology Research Group, Biotechnology Unit, Technology Development Center, Universidade Federal de Pelotas (UFPel), Pelotas, RS, BrazilMolecular and Cellular Oncology Research Group, Biotechnology Unit, Technology Development Center, Universidade Federal de Pelotas (UFPel), Pelotas, RS, BrazilMolecular and Cellular Oncology Research Group, Biotechnology Unit, Technology Development Center, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; Post-Graduate Programme in Biotechnology, CDTec, Universidade Federal de Pelotas (UFPel), Pelotas, RS, BrazilMolecular and Cellular Oncology Research Group, Biotechnology Unit, Technology Development Center, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; Post-Graduate Programme in Biotechnology, CDTec, Universidade Federal de Pelotas (UFPel), Pelotas, RS, BrazilPost-Graduate Programme in Epidemiology, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; Maternal and Child Department, Faculty of Medicine, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; Corresponding author at: Centro de Pesquisas Epidemiológicas, Rua Marechal Deodoro, 1160 – 3° Piso, Bairro Centro, Cep: 96020-220, Caixa Postal 464, Pelotas City, RS, Brazil.Background: It is believed that Human Papillomavirus (HPV) and Human Immunodeficiency Virus coinfection contributes to increase the risk for cervical intraepithelial injuries. Several factors may contribute to cervical cancer (CC) development, including genetic variants such as TP53 and MDM2 gene polymorphisms. Materials and methods: A hundred HIV-infected women were examined for HPV detection and its genotypes, as well as the frequencies of the SNPs Arg72Pro and SNP309 and their associations with CC risk factors. Nested Polymerase Chain Reaction (nPCR) was used for HPV detection and PCR-RFLP for TP53 and MDM2 SNP309 genotyping. Results: HPV DNA was detected in 68% of samples. A higher frequency of low-risk HPV genotypes (66.7%) was observed when compared to high-risk genotypes (33.3%). Nine different HPV genotypes were identified, with the highest prevalence of HPV-6, followed by HPV-16 and 31. p53 Arg72Arg and SNP309 TG genotype were the most prevalent. HPV genotyping was performed by sequencing. Conclusion: The data obtained suggest that HIV-infected women are more susceptible to be infected by low-risk HPV (LR-HPV) genotypes than by high-risk (HR-HPV), and Pro72Pro of TP53 gene and TG of MDM2 SNP309 genotypes apparently seem to be protective factors among HIV-infected women for HPV acquisition and HR-HPV infection, respectively, in a sample of Southern Brazilian woman. Future investigations in larger populations are necessary to better understand the potential roles of these SNPs and the behavior of non-oncogenic HPV genotypes in HIV-mediated immunosuppression cases. Keywords: HPV infection, HIV infection, Cervical cancer, Epidemiologyhttp://www.sciencedirect.com/science/article/pii/S1413867014001706 |
| spellingShingle | Ludmila Gonçalves Entiauspe Fabiana Kömmling Seixas Emily Montosa Nunes Fernanda Martins Rodrigues Odir A. Dellagostin Tiago Collares Mariângela Freitas da Silveira Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil Brazilian Journal of Infectious Diseases |
| title | Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil |
| title_full | Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil |
| title_fullStr | Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil |
| title_full_unstemmed | Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil |
| title_short | Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil |
| title_sort | uncommon non oncogenic hpv genotypes tp53 and mdm2 genes polymorphisms in hiv infected women in southern brazil |
| url | http://www.sciencedirect.com/science/article/pii/S1413867014001706 |
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