Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast Cancer
Background: Breast cancer is the most common malignancy among women. It is estimated that 1 in 10 women worldwide is affected by breast cancer during their lifetime. In 5 to 10% of breast cancer patients, the disease results from a hereditary predisposition, which can be attributable to mutations in...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kaviani Breast Disease Institute
2016-03-01
|
| Series: | Archives of Breast Cancer |
| Subjects: | |
| Online Access: | https://archbreastcancer.com/index.php/abc/article/view/51 |
| _version_ | 1818056611703291904 |
|---|---|
| author | Lamia Elfandi Ghada Said Saleh Suleiman Saleh Mohamed Marwan Nabil Enattah |
| author_facet | Lamia Elfandi Ghada Said Saleh Suleiman Saleh Mohamed Marwan Nabil Enattah |
| author_sort | Lamia Elfandi |
| collection | DOAJ |
| description | Background: Breast cancer is the most common malignancy among women. It is estimated that 1 in 10 women worldwide is affected by breast cancer during their lifetime. In 5 to 10% of breast cancer patients, the disease results from a hereditary predisposition, which can be attributable to mutations in either of two tumor suppressor genes, BRCA1 and BRCA2 to a large extent. BRCA2 6174delT mutation constitutes the common mutant alleles which predispose to hereditary breast cancer in the Ashkenazi population with a reported carrier frequency of 1.52%. In this study, we investigated the presence of the 6174delT mutation of the BRCA2 gene in Libyan woman affected with breast cancer and compared the results with those of other population groups.
Methods: Eighty- five Libyan women with breast cancer in additions to 5 relatives of the patients (healthy individuals) were recruited to this study. We obtained clinical information, family history, and peripheral blood for DNA extraction and analyzed the data using multiplex mutagenic polymerase chain reaction (MS-PCR) for detection of 6174delT mutation in the BRCA2 gene.
Results: The 6174delT of the BRCA2 gene was not detected either in the 85 patients with breast cancer (18 with familial breast cancer and 67 with sporadic breast cancer) nor in the 5 healthy individuals.
Conclusions: The present study showed that the 6174delT of the BRCA2 gene was not detectable using mutagenic PCR in the Libyan patients with breast cancer and can be considered to be exceedingly rare |
| first_indexed | 2024-12-10T12:31:36Z |
| format | Article |
| id | doaj.art-f7968aa8943a4b3eb475fa735c9f4ffc |
| institution | Directory Open Access Journal |
| issn | 2383-0433 |
| language | English |
| last_indexed | 2024-12-10T12:31:36Z |
| publishDate | 2016-03-01 |
| publisher | Kaviani Breast Disease Institute |
| record_format | Article |
| series | Archives of Breast Cancer |
| spelling | doaj.art-f7968aa8943a4b3eb475fa735c9f4ffc2022-12-22T01:48:49ZengKaviani Breast Disease InstituteArchives of Breast Cancer2383-04332016-03-013110.19187/abc.2016318-1350Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast CancerLamia Elfandi0Ghada Said1Saleh Suleiman Saleh2Mohamed Marwan3Nabil Enattah4School of Biological Sciences, The Libyan Academy, Tripoli, LibyaDepartment of Genetic Engineering, Biotechnology Research Center (BTRC), Twisha, Tripoli, LibyaDepartment of Genetic Engineering, Biotechnology Research Center (BTRC), Twisha, Tripoli, LibyaFaculty of Sciences, Tripoli University, Tripoli, LibyaDepartment of Genetic Engineering, Biotechnology Research Center (BTRC), Twisha, Tripoli, LibyaBackground: Breast cancer is the most common malignancy among women. It is estimated that 1 in 10 women worldwide is affected by breast cancer during their lifetime. In 5 to 10% of breast cancer patients, the disease results from a hereditary predisposition, which can be attributable to mutations in either of two tumor suppressor genes, BRCA1 and BRCA2 to a large extent. BRCA2 6174delT mutation constitutes the common mutant alleles which predispose to hereditary breast cancer in the Ashkenazi population with a reported carrier frequency of 1.52%. In this study, we investigated the presence of the 6174delT mutation of the BRCA2 gene in Libyan woman affected with breast cancer and compared the results with those of other population groups. Methods: Eighty- five Libyan women with breast cancer in additions to 5 relatives of the patients (healthy individuals) were recruited to this study. We obtained clinical information, family history, and peripheral blood for DNA extraction and analyzed the data using multiplex mutagenic polymerase chain reaction (MS-PCR) for detection of 6174delT mutation in the BRCA2 gene. Results: The 6174delT of the BRCA2 gene was not detected either in the 85 patients with breast cancer (18 with familial breast cancer and 67 with sporadic breast cancer) nor in the 5 healthy individuals. Conclusions: The present study showed that the 6174delT of the BRCA2 gene was not detectable using mutagenic PCR in the Libyan patients with breast cancer and can be considered to be exceedingly rarehttps://archbreastcancer.com/index.php/abc/article/view/51BRCA2 geneBreast cancerPolymorphisms6174delT |
| spellingShingle | Lamia Elfandi Ghada Said Saleh Suleiman Saleh Mohamed Marwan Nabil Enattah Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast Cancer Archives of Breast Cancer BRCA2 gene Breast cancer Polymorphisms 6174delT |
| title | Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast Cancer |
| title_full | Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast Cancer |
| title_fullStr | Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast Cancer |
| title_full_unstemmed | Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast Cancer |
| title_short | Analysis of 6174delT Mutation in BRCA2 Gene by Mutagenically Separated PCR Among Libyan Patients with Breast Cancer |
| title_sort | analysis of 6174delt mutation in brca2 gene by mutagenically separated pcr among libyan patients with breast cancer |
| topic | BRCA2 gene Breast cancer Polymorphisms 6174delT |
| url | https://archbreastcancer.com/index.php/abc/article/view/51 |
| work_keys_str_mv | AT lamiaelfandi analysisof6174deltmutationinbrca2genebymutagenicallyseparatedpcramonglibyanpatientswithbreastcancer AT ghadasaid analysisof6174deltmutationinbrca2genebymutagenicallyseparatedpcramonglibyanpatientswithbreastcancer AT salehsuleimansaleh analysisof6174deltmutationinbrca2genebymutagenicallyseparatedpcramonglibyanpatientswithbreastcancer AT mohamedmarwan analysisof6174deltmutationinbrca2genebymutagenicallyseparatedpcramonglibyanpatientswithbreastcancer AT nabilenattah analysisof6174deltmutationinbrca2genebymutagenicallyseparatedpcramonglibyanpatientswithbreastcancer |