Methanogenic toxicity evaluation of chlortetracycline hydrochloride
Background: Anaerobic digestion is a technology applied successfully to converting organic matter into biogas. However, the presence of inhibitory compounds such as antibiotics can adversely affect methane production. The aim of this study is to evaluate the toxic effect of chlortetracycline hydroch...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-11-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345815001281 |
| _version_ | 1818512707043721216 |
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| author | Carolina Reyes-Contreras Gladys Vidal |
| author_facet | Carolina Reyes-Contreras Gladys Vidal |
| author_sort | Carolina Reyes-Contreras |
| collection | DOAJ |
| description | Background: Anaerobic digestion is a technology applied successfully to converting organic matter into biogas. However, the presence of inhibitory compounds such as antibiotics can adversely affect methane production. The aim of this study is to evaluate the toxic effect of chlortetracycline hydrochloride (CLOR) on the methanogenic bacteria. In order to study the methanogenic toxicity of CLOR, different concentrations of CLOR (10, 50, 100, 200 mg L−1) were evaluated by methanogenic toxicity assays using three feedings.
Results: Maximum methane production was obtained for the assays with 10 mg CLOR L−1, the values obtained were 277 ± 4.07; 193 ± 11.31 and 166 ± 7.07 mL for the first, second and third feedings, respectively. The average values for acetic, propionic and butyric acid at start of the experiments were 2104 ± 139; 632 ± 7.6; 544 ± 26 mg L−1, respectively. The VFA values obtained finally of the experiment were dependent on the evaluated antibiotic concentrations, indicating that the efficiency of methanogenesis is directly affected by the CLOR concentration.
Conclusions: CLOR is an effective methanogenic bacteria inhibitor. Moreover, the results show that CLOR has a bactericidal effect on methanogenic activity given that methane production did not recover during the third feeding. This study shows that the 50% inhibitory concentration (IC50) for methanogenic bacteria in 10 mg L−1. |
| first_indexed | 2024-12-10T23:50:41Z |
| format | Article |
| id | doaj.art-f79af91a9be64be5aa5b1673d3c4a5b5 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-10T23:50:41Z |
| publishDate | 2015-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-f79af91a9be64be5aa5b1673d3c4a5b52022-12-22T01:28:47ZengElsevierElectronic Journal of Biotechnology0717-34582015-11-0118644545010.1016/j.ejbt.2015.09.009Methanogenic toxicity evaluation of chlortetracycline hydrochlorideCarolina Reyes-ContrerasGladys VidalBackground: Anaerobic digestion is a technology applied successfully to converting organic matter into biogas. However, the presence of inhibitory compounds such as antibiotics can adversely affect methane production. The aim of this study is to evaluate the toxic effect of chlortetracycline hydrochloride (CLOR) on the methanogenic bacteria. In order to study the methanogenic toxicity of CLOR, different concentrations of CLOR (10, 50, 100, 200 mg L−1) were evaluated by methanogenic toxicity assays using three feedings. Results: Maximum methane production was obtained for the assays with 10 mg CLOR L−1, the values obtained were 277 ± 4.07; 193 ± 11.31 and 166 ± 7.07 mL for the first, second and third feedings, respectively. The average values for acetic, propionic and butyric acid at start of the experiments were 2104 ± 139; 632 ± 7.6; 544 ± 26 mg L−1, respectively. The VFA values obtained finally of the experiment were dependent on the evaluated antibiotic concentrations, indicating that the efficiency of methanogenesis is directly affected by the CLOR concentration. Conclusions: CLOR is an effective methanogenic bacteria inhibitor. Moreover, the results show that CLOR has a bactericidal effect on methanogenic activity given that methane production did not recover during the third feeding. This study shows that the 50% inhibitory concentration (IC50) for methanogenic bacteria in 10 mg L−1.http://www.sciencedirect.com/science/article/pii/S0717345815001281AntibioticsAnaerobic batch assayBacterial inhibitionVolatile fatty acids |
| spellingShingle | Carolina Reyes-Contreras Gladys Vidal Methanogenic toxicity evaluation of chlortetracycline hydrochloride Electronic Journal of Biotechnology Antibiotics Anaerobic batch assay Bacterial inhibition Volatile fatty acids |
| title | Methanogenic toxicity evaluation of chlortetracycline hydrochloride |
| title_full | Methanogenic toxicity evaluation of chlortetracycline hydrochloride |
| title_fullStr | Methanogenic toxicity evaluation of chlortetracycline hydrochloride |
| title_full_unstemmed | Methanogenic toxicity evaluation of chlortetracycline hydrochloride |
| title_short | Methanogenic toxicity evaluation of chlortetracycline hydrochloride |
| title_sort | methanogenic toxicity evaluation of chlortetracycline hydrochloride |
| topic | Antibiotics Anaerobic batch assay Bacterial inhibition Volatile fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S0717345815001281 |
| work_keys_str_mv | AT carolinareyescontreras methanogenictoxicityevaluationofchlortetracyclinehydrochloride AT gladysvidal methanogenictoxicityevaluationofchlortetracyclinehydrochloride |