Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samples
To circumvent the problem of radiation damage when using an x-ray coherent diffraction imaging experiment to resolve the structure of biological samples, we propose a method to add objects made of heavy atoms with the bio-samples or we load the samples on a template made of heavy atoms. This templat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2014-01-01
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| Series: | New Journal of Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1367-2630/16/3/033016 |
| _version_ | 1797751549904027648 |
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| author | Ti-Yen Lan Po-Nan Li Ting-Kuo Lee |
| author_facet | Ti-Yen Lan Po-Nan Li Ting-Kuo Lee |
| author_sort | Ti-Yen Lan |
| collection | DOAJ |
| description | To circumvent the problem of radiation damage when using an x-ray coherent diffraction imaging experiment to resolve the structure of biological samples, we propose a method to add objects made of heavy atoms with the bio-samples or we load the samples on a template made of heavy atoms. This template method is shown by a numerical simulation (including shot noise) to be able to resolve the structure of a virus better than without the template. A counter-intuitive result is obtained, where heavier templates have a better resolution, even if the diffraction intensity of the bio-sample is much smaller than the noise intensity. In addition, the method also helps to greatly increase the efficiency of phase retrieval. We also provide a way to estimate the error to be expected if a particular experimental setting were chosen once the charge ratio between the sample and the template is estimated. Hence, this method will also help experiments choose the optimal setting for the best resolution with minimal radiation damage. |
| first_indexed | 2024-03-12T16:50:08Z |
| format | Article |
| id | doaj.art-f79b4c310ebe43329a5a1dc6b724aae3 |
| institution | Directory Open Access Journal |
| issn | 1367-2630 |
| language | English |
| last_indexed | 2024-03-12T16:50:08Z |
| publishDate | 2014-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | New Journal of Physics |
| spelling | doaj.art-f79b4c310ebe43329a5a1dc6b724aae32023-08-08T11:24:42ZengIOP PublishingNew Journal of Physics1367-26302014-01-0116303301610.1088/1367-2630/16/3/033016Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samplesTi-Yen Lan0Po-Nan Li1Ting-Kuo Lee2Institute of Physics, Academia Sinica , 128 section 2, Academia Road, Nankang, Taipei 11529, Republic of ChinaInstitute of Physics, Academia Sinica , 128 section 2, Academia Road, Nankang, Taipei 11529, Republic of ChinaInstitute of Physics, Academia Sinica , 128 section 2, Academia Road, Nankang, Taipei 11529, Republic of ChinaTo circumvent the problem of radiation damage when using an x-ray coherent diffraction imaging experiment to resolve the structure of biological samples, we propose a method to add objects made of heavy atoms with the bio-samples or we load the samples on a template made of heavy atoms. This template method is shown by a numerical simulation (including shot noise) to be able to resolve the structure of a virus better than without the template. A counter-intuitive result is obtained, where heavier templates have a better resolution, even if the diffraction intensity of the bio-sample is much smaller than the noise intensity. In addition, the method also helps to greatly increase the efficiency of phase retrieval. We also provide a way to estimate the error to be expected if a particular experimental setting were chosen once the charge ratio between the sample and the template is estimated. Hence, this method will also help experiments choose the optimal setting for the best resolution with minimal radiation damage.https://doi.org/10.1088/1367-2630/16/3/033016coherent diffractive imaginglensless imagingiterative phase-retrieval algorithmsoversamplingtemplate method42.30.Rx |
| spellingShingle | Ti-Yen Lan Po-Nan Li Ting-Kuo Lee Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samples New Journal of Physics coherent diffractive imaging lensless imaging iterative phase-retrieval algorithms oversampling template method 42.30.Rx |
| title | Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samples |
| title_full | Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samples |
| title_fullStr | Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samples |
| title_full_unstemmed | Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samples |
| title_short | Method to enhance the resolution of x-ray coherent diffraction imaging for non-crystalline bio-samples |
| title_sort | method to enhance the resolution of x ray coherent diffraction imaging for non crystalline bio samples |
| topic | coherent diffractive imaging lensless imaging iterative phase-retrieval algorithms oversampling template method 42.30.Rx |
| url | https://doi.org/10.1088/1367-2630/16/3/033016 |
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