Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and Function
Transplantation of unpurified islets into the liver, unlike that of purified islets, causes portal hypertension and coagulopathy. The aim of this project was to determine the most suitable alternative site for transplantation of unpurified pancreatic islets in autotransplanted dogs. Twenty-five fema...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
1995-05-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979500400308 |
| _version_ | 1818137968474324992 |
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| author | Ismail H. Al-Abdullah M.S. Anil Kumar Dawn Kelly-Sullivan George M. Abouna |
| author_facet | Ismail H. Al-Abdullah M.S. Anil Kumar Dawn Kelly-Sullivan George M. Abouna |
| author_sort | Ismail H. Al-Abdullah |
| collection | DOAJ |
| description | Transplantation of unpurified islets into the liver, unlike that of purified islets, causes portal hypertension and coagulopathy. The aim of this project was to determine the most suitable alternative site for transplantation of unpurified pancreatic islets in autotransplanted dogs. Twenty-five female mongrel dogs were divided into 5 groups depending on the site of islet transplantation: liver (3), spleen (7), skeletal muscle (5), omental pouch (6), and renal subcapsule (4). Pancreatic digestion of the total pancreatectomized specimen was carried out by distension of the pancreas with 1.5 mg/mL collagenase suspended in 250 mL Hanks' balanced salt solution using a semiautomatic method. The total number of islets equivalent isolated from 25 dogs was 90948 ± 6053. Only islets > 60 μm in diameter were counted, and the mean islet equivalent transplanted per kg body wt was 6762 ± 429. Islet function was achieved with transplantation into spleen in 71%, omental pouch in 50%, and muscle in 20%, but none in the renal subcapsule or liver groups. Glucose tolerance test at 30 d showed a mean K Value (decline in glucose, %/min) of 1.94 ± 0.73,0.79 ± 0.15 and 1.02 in the splenic, omental pouch and muscle groups, respectively. All animals in the liver group, 2 from the splenic group, and 2 from the renal subcapsule group died of diffuse bleeding. Four out of 5 dogs in the muscle group developed necrosis at the site of transplantation and the islets never functioned. This study demonstrates that in dogs, spleen and omental pouch appear to be suitable sites for transplantation of unpurified islets. |
| first_indexed | 2024-12-11T10:04:44Z |
| format | Article |
| id | doaj.art-f79d3f4adeaf4a06a3bab2176abb44bd |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-11T10:04:44Z |
| publishDate | 1995-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-f79d3f4adeaf4a06a3bab2176abb44bd2022-12-22T01:12:01ZengSAGE PublishingCell Transplantation0963-68971555-38921995-05-01410.1177/096368979500400308Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and FunctionIsmail H. Al-Abdullah0M.S. Anil Kumar1Dawn Kelly-Sullivan2George M. Abouna3Division of Transplantation, Department of Surgery, M.S. 417, Broad and Vine, Hahnemann University, Philadelphia, PA 19102-1192 USADivision of Transplantation, Department of Surgery, M.S. 417, Broad and Vine, Hahnemann University, Philadelphia, PA 19102-1192 USADivision of Transplantation, Department of Surgery, M.S. 417, Broad and Vine, Hahnemann University, Philadelphia, PA 19102-1192 USADivision of Transplantation, Department of Surgery, M.S. 417, Broad and Vine, Hahnemann University, Philadelphia, PA 19102-1192 USATransplantation of unpurified islets into the liver, unlike that of purified islets, causes portal hypertension and coagulopathy. The aim of this project was to determine the most suitable alternative site for transplantation of unpurified pancreatic islets in autotransplanted dogs. Twenty-five female mongrel dogs were divided into 5 groups depending on the site of islet transplantation: liver (3), spleen (7), skeletal muscle (5), omental pouch (6), and renal subcapsule (4). Pancreatic digestion of the total pancreatectomized specimen was carried out by distension of the pancreas with 1.5 mg/mL collagenase suspended in 250 mL Hanks' balanced salt solution using a semiautomatic method. The total number of islets equivalent isolated from 25 dogs was 90948 ± 6053. Only islets > 60 μm in diameter were counted, and the mean islet equivalent transplanted per kg body wt was 6762 ± 429. Islet function was achieved with transplantation into spleen in 71%, omental pouch in 50%, and muscle in 20%, but none in the renal subcapsule or liver groups. Glucose tolerance test at 30 d showed a mean K Value (decline in glucose, %/min) of 1.94 ± 0.73,0.79 ± 0.15 and 1.02 in the splenic, omental pouch and muscle groups, respectively. All animals in the liver group, 2 from the splenic group, and 2 from the renal subcapsule group died of diffuse bleeding. Four out of 5 dogs in the muscle group developed necrosis at the site of transplantation and the islets never functioned. This study demonstrates that in dogs, spleen and omental pouch appear to be suitable sites for transplantation of unpurified islets.https://doi.org/10.1177/096368979500400308 |
| spellingShingle | Ismail H. Al-Abdullah M.S. Anil Kumar Dawn Kelly-Sullivan George M. Abouna Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and Function Cell Transplantation |
| title | Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and Function |
| title_full | Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and Function |
| title_fullStr | Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and Function |
| title_full_unstemmed | Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and Function |
| title_short | Site for Unpurified Islet Transplantation is an Important Parameter for Determination of the Outcome of Graft Survival and Function |
| title_sort | site for unpurified islet transplantation is an important parameter for determination of the outcome of graft survival and function |
| url | https://doi.org/10.1177/096368979500400308 |
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