Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging
Abstract Table-top extreme ultraviolet (EUV) microscopy offers unique opportunities for label-free investigation of biological samples. Here, we demonstrate ptychographic EUV imaging of two dried, unstained model specimens: germlings of a fungus (Aspergillus nidulans), and bacteria (Escherichia coli...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-01-01
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| Series: | PhotoniX |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43074-023-00084-6 |
| _version_ | 1811175723585503232 |
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| author | Chang Liu Wilhelm Eschen Lars Loetgering Daniel S. Penagos Molina Robert Klas Alexander Iliou Michael Steinert Sebastian Herkersdorf Alexander Kirsche Thomas Pertsch Falk Hillmann Jens Limpert Jan Rothhardt |
| author_facet | Chang Liu Wilhelm Eschen Lars Loetgering Daniel S. Penagos Molina Robert Klas Alexander Iliou Michael Steinert Sebastian Herkersdorf Alexander Kirsche Thomas Pertsch Falk Hillmann Jens Limpert Jan Rothhardt |
| author_sort | Chang Liu |
| collection | DOAJ |
| description | Abstract Table-top extreme ultraviolet (EUV) microscopy offers unique opportunities for label-free investigation of biological samples. Here, we demonstrate ptychographic EUV imaging of two dried, unstained model specimens: germlings of a fungus (Aspergillus nidulans), and bacteria (Escherichia coli) cells at 13.5 nm wavelength. We find that the EUV spectral region, which to date has not received much attention for biological imaging, offers sufficient penetration depths for the identification of intracellular features. By implementing a position-correlated ptychography approach, we demonstrate a millimeter-squared field of view enabled by infrared illumination combined with sub-60 nm spatial resolution achieved with EUV illumination on selected regions of interest. The strong element contrast at 13.5 nm wavelength enables the identification of the nanoscale material composition inside the specimens. Our work will advance and facilitate EUV imaging applications and enable further possibilities in life science. |
| first_indexed | 2024-04-10T19:41:31Z |
| format | Article |
| id | doaj.art-f79fa50748444df6a3ca621258d105ea |
| institution | Directory Open Access Journal |
| issn | 2662-1991 |
| language | English |
| last_indexed | 2024-04-10T19:41:31Z |
| publishDate | 2023-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | PhotoniX |
| spelling | doaj.art-f79fa50748444df6a3ca621258d105ea2023-01-29T12:21:13ZengSpringerOpenPhotoniX2662-19912023-01-014111510.1186/s43074-023-00084-6Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imagingChang Liu0Wilhelm Eschen1Lars Loetgering2Daniel S. Penagos Molina3Robert Klas4Alexander Iliou5Michael Steinert6Sebastian Herkersdorf7Alexander Kirsche8Thomas Pertsch9Falk Hillmann10Jens Limpert11Jan Rothhardt12Institute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaInstitute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaInstitute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaInstitute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaInstitute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaLeibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (Leibniz- HKI)Institute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaDepartment of Pharmaceutical Microbiology, Hans Knöll Institute (HKI), Friedrich-Schiller- University JenaInstitute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaInstitute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaLeibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (Leibniz- HKI)Institute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaInstitute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University JenaAbstract Table-top extreme ultraviolet (EUV) microscopy offers unique opportunities for label-free investigation of biological samples. Here, we demonstrate ptychographic EUV imaging of two dried, unstained model specimens: germlings of a fungus (Aspergillus nidulans), and bacteria (Escherichia coli) cells at 13.5 nm wavelength. We find that the EUV spectral region, which to date has not received much attention for biological imaging, offers sufficient penetration depths for the identification of intracellular features. By implementing a position-correlated ptychography approach, we demonstrate a millimeter-squared field of view enabled by infrared illumination combined with sub-60 nm spatial resolution achieved with EUV illumination on selected regions of interest. The strong element contrast at 13.5 nm wavelength enables the identification of the nanoscale material composition inside the specimens. Our work will advance and facilitate EUV imaging applications and enable further possibilities in life science.https://doi.org/10.1186/s43074-023-00084-6EUVPtychographyMicroorganismHHG |
| spellingShingle | Chang Liu Wilhelm Eschen Lars Loetgering Daniel S. Penagos Molina Robert Klas Alexander Iliou Michael Steinert Sebastian Herkersdorf Alexander Kirsche Thomas Pertsch Falk Hillmann Jens Limpert Jan Rothhardt Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging PhotoniX EUV Ptychography Microorganism HHG |
| title | Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging |
| title_full | Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging |
| title_fullStr | Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging |
| title_full_unstemmed | Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging |
| title_short | Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging |
| title_sort | visualizing the ultra structure of microorganisms using table top extreme ultraviolet imaging |
| topic | EUV Ptychography Microorganism HHG |
| url | https://doi.org/10.1186/s43074-023-00084-6 |
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