Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing
Abstract We designed and developed two new types of hydrogen fuel cell (HFC) buses (motorcoach and minibus) with a mobile laboratory system. Feasibility studies have been performed for mobile laboratory testing, particularly for the laboratory performance of COVID-19 RT-PCR (PCR). We evaluated the d...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44925-7 |
| _version_ | 1797453176979324928 |
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| author | Miho Okude Kenji Suzuki Asami Naito Akio Ebashi Tomoka Kusama Junichi Kiyotaki Yusaku Akashi Yoshihiko Kiyasu Yoko Kurihara Shigeyuki Notake Masaki Takanashi Tomokazu Setoyama Yasushi Kawakami Hiromichi Suzuki |
| author_facet | Miho Okude Kenji Suzuki Asami Naito Akio Ebashi Tomoka Kusama Junichi Kiyotaki Yusaku Akashi Yoshihiko Kiyasu Yoko Kurihara Shigeyuki Notake Masaki Takanashi Tomokazu Setoyama Yasushi Kawakami Hiromichi Suzuki |
| author_sort | Miho Okude |
| collection | DOAJ |
| description | Abstract We designed and developed two new types of hydrogen fuel cell (HFC) buses (motorcoach and minibus) with a mobile laboratory system. Feasibility studies have been performed for mobile laboratory testing, particularly for the laboratory performance of COVID-19 RT-PCR (PCR). We evaluated the driving range capability, PCR sample size capacity, turnaround time (TAT), and analytical performance for the detection of SARS-CoV-2. Saliva samples were used for the current study, and the analytical performance was compared with that of the reference PCR. The estimated driving range and sample size capacity of the HFC and HFC minibus were 432 km and 2847 samples, respectively, for the HFC motorcoach and 313 km and 1949 samples for the HFC minibus. For the TAT, the median time between sample submission and completion of PCR was 86 min for the motorcoach and 76 min for the minibus, and the median time between sample submission and electronic reporting of the result to each visitor was 182 min for the motorcoach and 194 min for the minibus. A secondary analysis of 1574 HFC mobile laboratory testing samples was conducted, and all negative samples were found to be negative by reference PCR. Furthermore, all samples were confirmed to be positive by reference PCR or other molecular examinations. |
| first_indexed | 2024-03-09T15:19:04Z |
| format | Article |
| id | doaj.art-f7a10aa449034bfaa1dd888ca1572274 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:19:04Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f7a10aa449034bfaa1dd888ca15722742023-11-26T12:56:03ZengNature PortfolioScientific Reports2045-23222023-10-011311810.1038/s41598-023-44925-7Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testingMiho Okude0Kenji Suzuki1Asami Naito2Akio Ebashi3Tomoka Kusama4Junichi Kiyotaki5Yusaku Akashi6Yoshihiko Kiyasu7Yoko Kurihara8Shigeyuki Notake9Masaki Takanashi10Tomokazu Setoyama11Yasushi Kawakami12Hiromichi Suzuki13LSI Medience CorporationCenter for Cybernics Research, University of TsukubaLSI Medience CorporationDepartment of Infectious Diseases, University of Tsukuba HospitalDepartment of Infectious Diseases, University of Tsukuba HospitalMiroku Medical Laboratory IncDepartment of Infectious Diseases, Faculty of Medicine, University of TsukubaDepartment of Infectious Diseases, University of Tsukuba HospitalDepartment of Infectious Diseases, University of Tsukuba HospitalDepartment of Clinical Laboratory, Tsukuba Medical Center HospitalLSI Medience CorporationLSI Medience CorporationDepartment of Laboratory Medicine, Faculty of Medicine, University of TsukubaDepartment of Infectious Diseases, University of Tsukuba HospitalAbstract We designed and developed two new types of hydrogen fuel cell (HFC) buses (motorcoach and minibus) with a mobile laboratory system. Feasibility studies have been performed for mobile laboratory testing, particularly for the laboratory performance of COVID-19 RT-PCR (PCR). We evaluated the driving range capability, PCR sample size capacity, turnaround time (TAT), and analytical performance for the detection of SARS-CoV-2. Saliva samples were used for the current study, and the analytical performance was compared with that of the reference PCR. The estimated driving range and sample size capacity of the HFC and HFC minibus were 432 km and 2847 samples, respectively, for the HFC motorcoach and 313 km and 1949 samples for the HFC minibus. For the TAT, the median time between sample submission and completion of PCR was 86 min for the motorcoach and 76 min for the minibus, and the median time between sample submission and electronic reporting of the result to each visitor was 182 min for the motorcoach and 194 min for the minibus. A secondary analysis of 1574 HFC mobile laboratory testing samples was conducted, and all negative samples were found to be negative by reference PCR. Furthermore, all samples were confirmed to be positive by reference PCR or other molecular examinations.https://doi.org/10.1038/s41598-023-44925-7 |
| spellingShingle | Miho Okude Kenji Suzuki Asami Naito Akio Ebashi Tomoka Kusama Junichi Kiyotaki Yusaku Akashi Yoshihiko Kiyasu Yoko Kurihara Shigeyuki Notake Masaki Takanashi Tomokazu Setoyama Yasushi Kawakami Hiromichi Suzuki Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing Scientific Reports |
| title | Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing |
| title_full | Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing |
| title_fullStr | Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing |
| title_full_unstemmed | Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing |
| title_short | Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing |
| title_sort | development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for covid 19 rt pcr testing |
| url | https://doi.org/10.1038/s41598-023-44925-7 |
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