Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig
Despite playing a key role in digestion, there is only a broad characterization of the spatiotemporal development of the three glandular regions of the stomach (cardiac, fundic and pyloric) in the weaned pig. Hence, the objective of this experiment was to explore the differential expression (DE) of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/7/473 |
| _version_ | 1797587272591212544 |
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| author | Dillon P. Kiernan John V. O’Doherty Kathryn Ruth Connolly Marion Ryan Torres Sweeney |
| author_facet | Dillon P. Kiernan John V. O’Doherty Kathryn Ruth Connolly Marion Ryan Torres Sweeney |
| author_sort | Dillon P. Kiernan |
| collection | DOAJ |
| description | Despite playing a key role in digestion, there is only a broad characterization of the spatiotemporal development of the three glandular regions of the stomach (cardiac, fundic and pyloric) in the weaned pig. Hence, the objective of this experiment was to explore the differential expression (DE) of a panel of key genes within the three glandular regions of the stomach. Eight pigs were sacrificed at d 8 post-weaning, and three mucosal samples were collected from each stomach’s glandular regions. The expression of a panel of genes were measured using QPCR. The true cardiac gland region was characterized by increased expression of <i>PIGR</i>, <i>OLFM4</i>, <i>CXCL8</i> and <i>MUC2</i> relative to the two other regions (<i>p</i> < 0.05). The fundic gland region was characterized by increased expression of <i>ATP4A</i>, C<i>LIC6</i>, <i>KCNQ1</i>, <i>HRH2</i>, <i>AQP4</i>, <i>HDC</i>, <i>CCKBR</i>, <i>CHIA</i>, <i>PGA5</i>, <i>GHRL</i> and <i>MBOAT4</i> compared to the two other regions (<i>p</i> < 0.05). The pyloric gland region was characterized by exclusive expression of <i>GAST</i> (<i>p</i> < 0.05). A transition region between the cardiac and fundic region (cardiac-to-oxyntic transition) was observed with a gene expression signature that resembles a cross of the signatures found in the two regions. In conclusion, unique gene expression signatures were identifiable in each of the glandular regions, with a cardiac-to-oxyntic transition region clearly identifiable in the post-weaned pigs’ stomachs. |
| first_indexed | 2024-03-11T00:34:47Z |
| format | Article |
| id | doaj.art-f7a283338ac34f4f81832f0af972ede8 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T00:34:47Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-f7a283338ac34f4f81832f0af972ede82023-11-18T21:43:06ZengMDPI AGVeterinary Sciences2306-73812023-07-0110747310.3390/vetsci10070473Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned PigDillon P. Kiernan0John V. O’Doherty1Kathryn Ruth Connolly2Marion Ryan3Torres Sweeney4School of Veterinary Medicine, University College Dublin, Belfield, D04 W6F6 Dublin, IrelandSchool of Agriculture and Food Science, University College Dublin, Belfield, D04 W6F6 Dublin, IrelandSchool of Agriculture and Food Science, University College Dublin, Belfield, D04 W6F6 Dublin, IrelandSchool of Agriculture and Food Science, University College Dublin, Belfield, D04 W6F6 Dublin, IrelandSchool of Veterinary Medicine, University College Dublin, Belfield, D04 W6F6 Dublin, IrelandDespite playing a key role in digestion, there is only a broad characterization of the spatiotemporal development of the three glandular regions of the stomach (cardiac, fundic and pyloric) in the weaned pig. Hence, the objective of this experiment was to explore the differential expression (DE) of a panel of key genes within the three glandular regions of the stomach. Eight pigs were sacrificed at d 8 post-weaning, and three mucosal samples were collected from each stomach’s glandular regions. The expression of a panel of genes were measured using QPCR. The true cardiac gland region was characterized by increased expression of <i>PIGR</i>, <i>OLFM4</i>, <i>CXCL8</i> and <i>MUC2</i> relative to the two other regions (<i>p</i> < 0.05). The fundic gland region was characterized by increased expression of <i>ATP4A</i>, C<i>LIC6</i>, <i>KCNQ1</i>, <i>HRH2</i>, <i>AQP4</i>, <i>HDC</i>, <i>CCKBR</i>, <i>CHIA</i>, <i>PGA5</i>, <i>GHRL</i> and <i>MBOAT4</i> compared to the two other regions (<i>p</i> < 0.05). The pyloric gland region was characterized by exclusive expression of <i>GAST</i> (<i>p</i> < 0.05). A transition region between the cardiac and fundic region (cardiac-to-oxyntic transition) was observed with a gene expression signature that resembles a cross of the signatures found in the two regions. In conclusion, unique gene expression signatures were identifiable in each of the glandular regions, with a cardiac-to-oxyntic transition region clearly identifiable in the post-weaned pigs’ stomachs.https://www.mdpi.com/2306-7381/10/7/473monogastricacid secretionfundic gland regionpyloric gland regioncardiac gland regioncardiac-to-oxyntic transition |
| spellingShingle | Dillon P. Kiernan John V. O’Doherty Kathryn Ruth Connolly Marion Ryan Torres Sweeney Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig Veterinary Sciences monogastric acid secretion fundic gland region pyloric gland region cardiac gland region cardiac-to-oxyntic transition |
| title | Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig |
| title_full | Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig |
| title_fullStr | Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig |
| title_full_unstemmed | Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig |
| title_short | Exploring the Differential Expression of a Set of Key Genes Involved in the Regulation and Functioning of the Stomach in the Post-Weaned Pig |
| title_sort | exploring the differential expression of a set of key genes involved in the regulation and functioning of the stomach in the post weaned pig |
| topic | monogastric acid secretion fundic gland region pyloric gland region cardiac gland region cardiac-to-oxyntic transition |
| url | https://www.mdpi.com/2306-7381/10/7/473 |
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