Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein
"nBackground: Cryptosporidium parvum is a parasitic protozoan that functions as important causative agent of diarrhea in human and animals. The host's immune response to surface antigens of C. parvum has been previously demonstrated. In this respect, the role of humoral immunity in...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2009-07-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14220.pdf&manuscript_id=14220 |
| _version_ | 1819280641498284032 |
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| author | S Rahbari E Ebrahimzadeh P Shayan P Shahbazi |
| author_facet | S Rahbari E Ebrahimzadeh P Shayan P Shahbazi |
| author_sort | S Rahbari |
| collection | DOAJ |
| description | "nBackground: Cryptosporidium parvum is a parasitic protozoan that functions as important causative agent of diarrhea in human and animals. The host's immune response to surface antigens of C. parvum has been previously demonstrated. In this respect, the role of humoral immunity in the development of host protective immunity against this protozoon has been well demonstrated. "nMethods: The effect of specific chicken egg yolk antibody (IgY) against recombinant C. parvum P23 was examined. IgY sample was prepared from eggs of chickens immunized with recombinant C. parvum protein p23 and analyzed with C. parvum lysate and recombinant P23. "nResults: The anti P23 specific IgY was recognized a protein band with approximately 23 kDa in lysates prepared from the C. parvum oocysts. Also dot blot analysis of recombinant P23 showed that it could be recognized by the anti P23 specific IgY up to 1/1000 dilution of antibody. But the best antibody dilution for immunological studies was determined as 1:200. "nConclusion: Since P23 is an immunodominant surface glycoprotein expressed in the early phase of infection, specific IgY against recombinant p23 could be recommended as a favorable candidate for passive immunization against C. parvum infection in human and animals. |
| first_indexed | 2024-12-24T00:47:02Z |
| format | Article |
| id | doaj.art-f7a356df385744bc88f311caa437ae68 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-24T00:47:02Z |
| publishDate | 2009-07-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-f7a356df385744bc88f311caa437ae682022-12-21T17:23:46ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2009-07-01431524Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 ProteinS RahbariE EbrahimzadehP ShayanP Shahbazi"nBackground: Cryptosporidium parvum is a parasitic protozoan that functions as important causative agent of diarrhea in human and animals. The host's immune response to surface antigens of C. parvum has been previously demonstrated. In this respect, the role of humoral immunity in the development of host protective immunity against this protozoon has been well demonstrated. "nMethods: The effect of specific chicken egg yolk antibody (IgY) against recombinant C. parvum P23 was examined. IgY sample was prepared from eggs of chickens immunized with recombinant C. parvum protein p23 and analyzed with C. parvum lysate and recombinant P23. "nResults: The anti P23 specific IgY was recognized a protein band with approximately 23 kDa in lysates prepared from the C. parvum oocysts. Also dot blot analysis of recombinant P23 showed that it could be recognized by the anti P23 specific IgY up to 1/1000 dilution of antibody. But the best antibody dilution for immunological studies was determined as 1:200. "nConclusion: Since P23 is an immunodominant surface glycoprotein expressed in the early phase of infection, specific IgY against recombinant p23 could be recommended as a favorable candidate for passive immunization against C. parvum infection in human and animals.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14220.pdf&manuscript_id=14220 Cryptosporidium parvumIgYPCRRecombinant protein p23Western Blot |
| spellingShingle | S Rahbari E Ebrahimzadeh P Shayan P Shahbazi Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein Iranian Journal of Parasitology Cryptosporidium parvum IgY PCR Recombinant protein p23 Western Blot |
| title | Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein |
| title_full | Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein |
| title_fullStr | Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein |
| title_full_unstemmed | Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein |
| title_short | Specific Egg Yolk Antibody against Recombinant Cryptosporidium parvum P23 Protein |
| title_sort | specific egg yolk antibody against recombinant cryptosporidium parvum p23 protein |
| topic | Cryptosporidium parvum IgY PCR Recombinant protein p23 Western Blot |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/14220.pdf&manuscript_id=14220 |
| work_keys_str_mv | AT srahbari specificeggyolkantibodyagainstrecombinantcryptosporidiumparvump23protein AT eebrahimzadeh specificeggyolkantibodyagainstrecombinantcryptosporidiumparvump23protein AT pshayan specificeggyolkantibodyagainstrecombinantcryptosporidiumparvump23protein AT pshahbazi specificeggyolkantibodyagainstrecombinantcryptosporidiumparvump23protein |