Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak.
Between November 2010, and May 2011, eleven cases of cholera, unrelated to a concurrent outbreak on the island of Hispaniola, were recorded, and the causative agent, Vibrio cholerae serogroup O75, was traced to oysters harvested from Apalachicola Bay, Florida. From the 11 diagnosed cases, eight isol...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0086264&type=printable |
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| author | Bradd J Haley Seon Young Choi Christopher J Grim Tiffiani J Onifade Hediye N Cinar Ben D Tall Elisa Taviani Nur A Hasan Abdulshakur H Abdullah Laurenda Carter Surasri N Sahu Mahendra H Kothary Arlene Chen Ron Baker Richard Hutchinson Carina Blackmore Thomas A Cebula Anwar Huq Rita R Colwell |
| author_facet | Bradd J Haley Seon Young Choi Christopher J Grim Tiffiani J Onifade Hediye N Cinar Ben D Tall Elisa Taviani Nur A Hasan Abdulshakur H Abdullah Laurenda Carter Surasri N Sahu Mahendra H Kothary Arlene Chen Ron Baker Richard Hutchinson Carina Blackmore Thomas A Cebula Anwar Huq Rita R Colwell |
| author_sort | Bradd J Haley |
| collection | DOAJ |
| description | Between November 2010, and May 2011, eleven cases of cholera, unrelated to a concurrent outbreak on the island of Hispaniola, were recorded, and the causative agent, Vibrio cholerae serogroup O75, was traced to oysters harvested from Apalachicola Bay, Florida. From the 11 diagnosed cases, eight isolates of V. cholerae were isolated and their genomes were sequenced. Genomic analysis demonstrated the presence of a suite of mobile elements previously shown to be involved in the disease process of cholera (ctxAB, VPI-1 and -2, and a VSP-II like variant) and a phylogenomic analysis showed the isolates to be sister taxa to toxigenic V. cholerae V51 serogroup O141, a clinical strain isolated 23 years earlier. Toxigenic V. cholerae O75 has been repeatedly isolated from clinical cases in the southeastern United States and toxigenic V. cholerae O141 isolates have been isolated globally from clinical cases over several decades. Comparative genomics, phenotypic analyses, and a Caenorhabditis elegans model of infection for the isolates were conducted. This analysis coupled with isolation data of V. cholerae O75 and O141 suggests these strains may represent an underappreciated clade of cholera-causing strains responsible for significant disease burden globally. |
| first_indexed | 2024-12-22T01:07:06Z |
| format | Article |
| id | doaj.art-f7a3bb79d5c9474bb6cd2698f1aec51a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T16:17:42Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f7a3bb79d5c9474bb6cd2698f1aec51a2025-02-22T05:34:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e8626410.1371/journal.pone.0086264Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak.Bradd J HaleySeon Young ChoiChristopher J GrimTiffiani J OnifadeHediye N CinarBen D TallElisa TavianiNur A HasanAbdulshakur H AbdullahLaurenda CarterSurasri N SahuMahendra H KotharyArlene ChenRon BakerRichard HutchinsonCarina BlackmoreThomas A CebulaAnwar HuqRita R ColwellBetween November 2010, and May 2011, eleven cases of cholera, unrelated to a concurrent outbreak on the island of Hispaniola, were recorded, and the causative agent, Vibrio cholerae serogroup O75, was traced to oysters harvested from Apalachicola Bay, Florida. From the 11 diagnosed cases, eight isolates of V. cholerae were isolated and their genomes were sequenced. Genomic analysis demonstrated the presence of a suite of mobile elements previously shown to be involved in the disease process of cholera (ctxAB, VPI-1 and -2, and a VSP-II like variant) and a phylogenomic analysis showed the isolates to be sister taxa to toxigenic V. cholerae V51 serogroup O141, a clinical strain isolated 23 years earlier. Toxigenic V. cholerae O75 has been repeatedly isolated from clinical cases in the southeastern United States and toxigenic V. cholerae O141 isolates have been isolated globally from clinical cases over several decades. Comparative genomics, phenotypic analyses, and a Caenorhabditis elegans model of infection for the isolates were conducted. This analysis coupled with isolation data of V. cholerae O75 and O141 suggests these strains may represent an underappreciated clade of cholera-causing strains responsible for significant disease burden globally.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0086264&type=printable |
| spellingShingle | Bradd J Haley Seon Young Choi Christopher J Grim Tiffiani J Onifade Hediye N Cinar Ben D Tall Elisa Taviani Nur A Hasan Abdulshakur H Abdullah Laurenda Carter Surasri N Sahu Mahendra H Kothary Arlene Chen Ron Baker Richard Hutchinson Carina Blackmore Thomas A Cebula Anwar Huq Rita R Colwell Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak. PLoS ONE |
| title | Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak. |
| title_full | Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak. |
| title_fullStr | Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak. |
| title_full_unstemmed | Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak. |
| title_short | Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak. |
| title_sort | genomic and phenotypic characterization of vibrio cholerae non o1 isolates from a us gulf coast cholera outbreak |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0086264&type=printable |
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