Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance
MicroRNAs are a class of small regulatory RNAs in plants, which play vital roles during various abiotic and abiotic stress conditions including plant processes. In this present study, we examined the expression of miRNAs and their predicted target expression levels during heat stress in banana. Out...
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| Format: | Article |
| Language: | English |
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Society for Promotion of Horticulture - Indian Institute of Horticultural Research
2018-06-01
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| Series: | Journal of Horticultural Sciences |
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| Online Access: | https://jhs.iihr.res.in/index.php/jhs/article/view/35 |
| _version_ | 1797740692985872384 |
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| author | S M Vidya K V Ravishankar R H Laxman |
| author_facet | S M Vidya K V Ravishankar R H Laxman |
| author_sort | S M Vidya |
| collection | DOAJ |
| description |
MicroRNAs are a class of small regulatory RNAs in plants, which play vital roles during various abiotic and abiotic stress conditions including plant processes. In this present study, we examined the expression of miRNAs and their predicted target expression levels during heat stress in banana. Out of 235 miRNA found in Musa, 40 miRNA showed homology to heat responsive miRNAs from other plants. Further, 14 targets for miRNA were predicted that are potentially regulated by their cognate miRNAs and were monitored under three stages of stress viz, induction, induction + lethal alone using qPCR analysis. The results suggest that generally, there is a negative relationship in the expression patterns of miRNA and their predicted cognate targets - HSP70, HSP90, SAP, DNAj genes. These were highly up regulated and their respective miRNAs showed lower expression. This is the first report in banana, which demonstrated that during induction stress, various thermo-protective genes are activated at initial stages of stress to achieve thermotolerance through altered miRNA expression. The results will help in broadening our understanding acquired thermotolerance and their regulation by miRNAs in plants.
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| first_indexed | 2024-03-12T14:16:04Z |
| format | Article |
| id | doaj.art-f7a54375c0324229b6d98a14d8d80976 |
| institution | Directory Open Access Journal |
| issn | 0973-354X 2582-4899 |
| language | English |
| last_indexed | 2024-03-12T14:16:04Z |
| publishDate | 2018-06-01 |
| publisher | Society for Promotion of Horticulture - Indian Institute of Horticultural Research |
| record_format | Article |
| series | Journal of Horticultural Sciences |
| spelling | doaj.art-f7a54375c0324229b6d98a14d8d809762023-08-20T13:59:22ZengSociety for Promotion of Horticulture - Indian Institute of Horticultural ResearchJournal of Horticultural Sciences0973-354X2582-48992018-06-0113110.24154/jhs.v13i1.3529Genome wide analysis of heat responsive microRNAs in banana during acquired thermo toleranceS M VidyaK V RavishankarR H Laxman MicroRNAs are a class of small regulatory RNAs in plants, which play vital roles during various abiotic and abiotic stress conditions including plant processes. In this present study, we examined the expression of miRNAs and their predicted target expression levels during heat stress in banana. Out of 235 miRNA found in Musa, 40 miRNA showed homology to heat responsive miRNAs from other plants. Further, 14 targets for miRNA were predicted that are potentially regulated by their cognate miRNAs and were monitored under three stages of stress viz, induction, induction + lethal alone using qPCR analysis. The results suggest that generally, there is a negative relationship in the expression patterns of miRNA and their predicted cognate targets - HSP70, HSP90, SAP, DNAj genes. These were highly up regulated and their respective miRNAs showed lower expression. This is the first report in banana, which demonstrated that during induction stress, various thermo-protective genes are activated at initial stages of stress to achieve thermotolerance through altered miRNA expression. The results will help in broadening our understanding acquired thermotolerance and their regulation by miRNAs in plants. https://jhs.iihr.res.in/index.php/jhs/article/view/35Acquired thermo tolerance, banana, heat stress, miRNA,thermo protective genes. |
| spellingShingle | S M Vidya K V Ravishankar R H Laxman Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance Journal of Horticultural Sciences Acquired thermo tolerance, banana, heat stress, miRNA,thermo protective genes. |
| title | Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance |
| title_full | Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance |
| title_fullStr | Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance |
| title_full_unstemmed | Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance |
| title_short | Genome wide analysis of heat responsive microRNAs in banana during acquired thermo tolerance |
| title_sort | genome wide analysis of heat responsive micrornas in banana during acquired thermo tolerance |
| topic | Acquired thermo tolerance, banana, heat stress, miRNA,thermo protective genes. |
| url | https://jhs.iihr.res.in/index.php/jhs/article/view/35 |
| work_keys_str_mv | AT smvidya genomewideanalysisofheatresponsivemicrornasinbananaduringacquiredthermotolerance AT kvravishankar genomewideanalysisofheatresponsivemicrornasinbananaduringacquiredthermotolerance AT rhlaxman genomewideanalysisofheatresponsivemicrornasinbananaduringacquiredthermotolerance |