An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studies
Abstract We report anatomically correct 3D‐printed mouse phantoms that can be used to plan experiments and evaluate analysis protocols for magnetic particle imaging (MPI) studies. The 3D‐printed phantoms were based on the Digimouse 3D whole body mouse atlas and incorporate cavities representative of...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10299 |
| _version_ | 1798036076915326976 |
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| author | Nicole S. Sarna Leyda Marrero‐Morales Ryan DeGroff Angelie Rivera‐Rodriguez Sitong Liu Andreina Chiu‐Lam Hayden J. Good Carlos M. Rinaldi‐Ramos |
| author_facet | Nicole S. Sarna Leyda Marrero‐Morales Ryan DeGroff Angelie Rivera‐Rodriguez Sitong Liu Andreina Chiu‐Lam Hayden J. Good Carlos M. Rinaldi‐Ramos |
| author_sort | Nicole S. Sarna |
| collection | DOAJ |
| description | Abstract We report anatomically correct 3D‐printed mouse phantoms that can be used to plan experiments and evaluate analysis protocols for magnetic particle imaging (MPI) studies. The 3D‐printed phantoms were based on the Digimouse 3D whole body mouse atlas and incorporate cavities representative of a liver, brain tumor, and orthotopic breast cancer tumor placed in anatomically correct locations, allowing evaluation of the effect of precise doses of MPI tracer. To illustrate their use, a constant tracer iron mass was present in the liver for the breast (200 μgFe) and brain tumor (10 μgFe) model, respectively, while a series of decreasing tracer iron mass was placed in the tumor region. MPI scans were acquired in 2D and 3D high sensitivity and high sensitivity/high resolution (HSHR) modes using a MOMENTUM imager. A thresholding algorithm was used to define regions of interest (ROIs) in the scans and the tracer mass in the liver and tumors was calculated by comparison of the signal in their respective ROI against that of known mass fiducials that were included in each scan. The results demonstrate that this approach to image analysis provides accurate estimates of tracer mass. Additionally, the results show how the limit of detection in MPI is sensitive to the details of tracer distribution in the subject, as we found that a greater tracer mass in the liver cavity resulted in poorer sensitivity in tumor regions. These experiments illustrate the utility of the reported 3D‐printed anatomically correct mouse phantoms in evaluating methods to analyze MPI scans and plan in vivo experiments. |
| first_indexed | 2024-04-11T21:07:22Z |
| format | Article |
| id | doaj.art-f7a5d3ab8f164fd795bff0a3cc114682 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-04-11T21:07:22Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-f7a5d3ab8f164fd795bff0a3cc1146822022-12-22T04:03:15ZengWileyBioengineering & Translational Medicine2380-67612022-09-0173n/an/a10.1002/btm2.10299An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studiesNicole S. Sarna0Leyda Marrero‐Morales1Ryan DeGroff2Angelie Rivera‐Rodriguez3Sitong Liu4Andreina Chiu‐Lam5Hayden J. Good6Carlos M. Rinaldi‐Ramos7J. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville Florida USAJ. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville Florida USAJ. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville Florida USAJ. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville Florida USADepartment of Chemical Engineering University of Florida Gainesville Florida USADepartment of Chemical Engineering University of Florida Gainesville Florida USADepartment of Chemical Engineering University of Florida Gainesville Florida USAJ. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville Florida USAAbstract We report anatomically correct 3D‐printed mouse phantoms that can be used to plan experiments and evaluate analysis protocols for magnetic particle imaging (MPI) studies. The 3D‐printed phantoms were based on the Digimouse 3D whole body mouse atlas and incorporate cavities representative of a liver, brain tumor, and orthotopic breast cancer tumor placed in anatomically correct locations, allowing evaluation of the effect of precise doses of MPI tracer. To illustrate their use, a constant tracer iron mass was present in the liver for the breast (200 μgFe) and brain tumor (10 μgFe) model, respectively, while a series of decreasing tracer iron mass was placed in the tumor region. MPI scans were acquired in 2D and 3D high sensitivity and high sensitivity/high resolution (HSHR) modes using a MOMENTUM imager. A thresholding algorithm was used to define regions of interest (ROIs) in the scans and the tracer mass in the liver and tumors was calculated by comparison of the signal in their respective ROI against that of known mass fiducials that were included in each scan. The results demonstrate that this approach to image analysis provides accurate estimates of tracer mass. Additionally, the results show how the limit of detection in MPI is sensitive to the details of tracer distribution in the subject, as we found that a greater tracer mass in the liver cavity resulted in poorer sensitivity in tumor regions. These experiments illustrate the utility of the reported 3D‐printed anatomically correct mouse phantoms in evaluating methods to analyze MPI scans and plan in vivo experiments.https://doi.org/10.1002/btm2.102993D printinganimal replacementimaging phantommagnetic particle imaging |
| spellingShingle | Nicole S. Sarna Leyda Marrero‐Morales Ryan DeGroff Angelie Rivera‐Rodriguez Sitong Liu Andreina Chiu‐Lam Hayden J. Good Carlos M. Rinaldi‐Ramos An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studies Bioengineering & Translational Medicine 3D printing animal replacement imaging phantom magnetic particle imaging |
| title | An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studies |
| title_full | An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studies |
| title_fullStr | An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studies |
| title_full_unstemmed | An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studies |
| title_short | An anatomically correct 3D‐printed mouse phantom for magnetic particle imaging studies |
| title_sort | anatomically correct 3d printed mouse phantom for magnetic particle imaging studies |
| topic | 3D printing animal replacement imaging phantom magnetic particle imaging |
| url | https://doi.org/10.1002/btm2.10299 |
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