Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed Semen

The purpose of this study is to produce wanted sex progeny of genetically confined White Hanwoo (albinism) with preselected sex sperm. One bull of White Hanwoo was chosen for semen donor and X sperm was sorted by MoFlo XDP cell sorter. To compare the pregnancy and birth rates, KPN straw was used as...

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Main Authors: Sung Woo Kim, Jinseok Choi, Changyoung Choe, Dongkyo Kim, Yeoung-Gyu Ko, Chungsil So, Hwan-Hoo Seong
Format: Article
Language:English
Published: The Korean Society of Animal Reproduction and Biotechnology 2015-03-01
Series:Journal of Animal Reproduction and Biotechnology
Subjects:
Online Access:http://www.e-jarb.org/journal/view.html?uid=1463&vmd=Full
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author Sung Woo Kim
Jinseok Choi
Changyoung Choe
Dongkyo Kim
Yeoung-Gyu Ko
Chungsil So
Hwan-Hoo Seong
author_facet Sung Woo Kim
Jinseok Choi
Changyoung Choe
Dongkyo Kim
Yeoung-Gyu Ko
Chungsil So
Hwan-Hoo Seong
author_sort Sung Woo Kim
collection DOAJ
description The purpose of this study is to produce wanted sex progeny of genetically confined White Hanwoo (albinism) with preselected sex sperm. One bull of White Hanwoo was chosen for semen donor and X sperm was sorted by MoFlo XDP cell sorter. To compare the pregnancy and birth rates, KPN straw was used as control, total number of unsorted sperm was 20×106/straw. Sexed X frozen semen with 2×106 cells or 4×106 cells per straw were in seminated twice on Hanwoo heifers. The abnormality of the sexed X semen was 24.9 ± 7.31% and distal reflex abnormality of mid piece was significantly (p<0.05) higher (11.7%) compared with that of KPN 768 (5.6%). There were no differences on the pregnancy and birth rates between 2×106 cells or 4×106 cells of X-sperm but KPN semen showed significant differences (p<0.05). The pregnancy rates of KPN 768, 2×106 cells and 4×106 cells X-sperm of White Hanwoo cattle were 85.0%, 26.3% and 50%. The birth rates were 80.0%, 15.8% and 21.4%, respectively. The female offspring rates of KPN 768, 2×106 cells and 4×106 cells X-sperm of White Hanwoo cattle were 43.8%, 100% and 100% (p<0.05). These results indicated that sex sorted White Hanwoo could be used for the production of wanted progeny with 2×106 cells/straw for AI. To increase the efficiency of calf production, the sperm number of sex sorted semen will be optimized for sex selection of White Hanwoo progeny.
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spelling doaj.art-f7a62c4895ef4b13836d180a7833f20c2022-12-21T23:24:05ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632015-03-01301455010.12750/JET.2015.30.1.45Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed SemenSung Woo Kim0Jinseok Choi1Changyoung Choe2Dongkyo Kim3Yeoung-Gyu Ko4Chungsil So5Hwan-Hoo Seong6Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 590-832, KoreaThe purpose of this study is to produce wanted sex progeny of genetically confined White Hanwoo (albinism) with preselected sex sperm. One bull of White Hanwoo was chosen for semen donor and X sperm was sorted by MoFlo XDP cell sorter. To compare the pregnancy and birth rates, KPN straw was used as control, total number of unsorted sperm was 20×106/straw. Sexed X frozen semen with 2×106 cells or 4×106 cells per straw were in seminated twice on Hanwoo heifers. The abnormality of the sexed X semen was 24.9 ± 7.31% and distal reflex abnormality of mid piece was significantly (p<0.05) higher (11.7%) compared with that of KPN 768 (5.6%). There were no differences on the pregnancy and birth rates between 2×106 cells or 4×106 cells of X-sperm but KPN semen showed significant differences (p<0.05). The pregnancy rates of KPN 768, 2×106 cells and 4×106 cells X-sperm of White Hanwoo cattle were 85.0%, 26.3% and 50%. The birth rates were 80.0%, 15.8% and 21.4%, respectively. The female offspring rates of KPN 768, 2×106 cells and 4×106 cells X-sperm of White Hanwoo cattle were 43.8%, 100% and 100% (p<0.05). These results indicated that sex sorted White Hanwoo could be used for the production of wanted progeny with 2×106 cells/straw for AI. To increase the efficiency of calf production, the sperm number of sex sorted semen will be optimized for sex selection of White Hanwoo progeny.http://www.e-jarb.org/journal/view.html?uid=1463&vmd=Fullalbino korea native cattlesexed semenai
spellingShingle Sung Woo Kim
Jinseok Choi
Changyoung Choe
Dongkyo Kim
Yeoung-Gyu Ko
Chungsil So
Hwan-Hoo Seong
Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed Semen
Journal of Animal Reproduction and Biotechnology
albino korea native cattle
sexed semen
ai
title Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed Semen
title_full Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed Semen
title_fullStr Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed Semen
title_full_unstemmed Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed Semen
title_short Studies on the Estimation of Pregnancy Rate of White Hanwoo (Albino Korean Native Cattle) with Sexed Semen
title_sort studies on the estimation of pregnancy rate of white hanwoo albino korean native cattle with sexed semen
topic albino korea native cattle
sexed semen
ai
url http://www.e-jarb.org/journal/view.html?uid=1463&vmd=Full
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