Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiota
In this study, we exposed adult male crayfish (Procambarus clarkii) to different concentrations of diclofenac (DCF) for 96 h. In the meantime, we investigated the alternations of hepatopancreatic pathology, molecular regulation and intestinal microbiota of P. clarkii exposed to DCF. The results demo...
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| Format: | Article |
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Elsevier
2022-10-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322009083 |
| _version_ | 1798031443655393280 |
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| author | Yu Zhang Zheyu Li Yanxiang Zhang Kai Sun Nanqi Ren Mingtang Li |
| author_facet | Yu Zhang Zheyu Li Yanxiang Zhang Kai Sun Nanqi Ren Mingtang Li |
| author_sort | Yu Zhang |
| collection | DOAJ |
| description | In this study, we exposed adult male crayfish (Procambarus clarkii) to different concentrations of diclofenac (DCF) for 96 h. In the meantime, we investigated the alternations of hepatopancreatic pathology, molecular regulation and intestinal microbiota of P. clarkii exposed to DCF. The results demonstrated DCF led to histological changes including epithelium vacuolization and tubule lumen dilatation in the hepatopancreas. Transcriptome sequencing analysis showed that 642 and 586 genes were differentially expressed in the hepatopancreas of P. clarkii exposed to 1 and 10 mg/L DCF, respectively. DCF could affect the functions of antioxidation, immunity and metabolism of hepatopancreas by inducing the abnormal expressions of immune- and redox-related genes. GO enrichment results demonstrated that 10 mg/L DCF exposure could modulate the processes of molting, amino sugar metabolism, protein hydrolysis and intracellular protein translocation of P. clarkii. Additionally, the abundances of bacterial families including Shewanellaceae, Bacteroidaceae, Vibrionaceae, Erysipelotrichaceae, Aeromonadaceae, Moraxellaceae, etc. in the intestine were significantly changed after DCF exposure, and the disruption of intestinal flora might further cause abnormal intestinal metabolism in P. clarkii. This study provides novel mechanistic insights into the toxic effects of anti-inflammatory drugs on aquatic crustaceans. |
| first_indexed | 2024-04-11T19:57:34Z |
| format | Article |
| id | doaj.art-f7a8efd2e74b4e2889f5e37586a7700b |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T19:57:34Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-f7a8efd2e74b4e2889f5e37586a7700b2022-12-22T04:05:58ZengElsevierEcotoxicology and Environmental Safety0147-65132022-10-01244114068Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiotaYu Zhang0Zheyu Li1Yanxiang Zhang2Kai Sun3Nanqi Ren4Mingtang Li5Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, College of Resources and Environment, Jilin Agricultural University, Changchun 130118, ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, ChinaSchool of Environmental and Material Engineering, Yantai University, Yantai 264005, ChinaState Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China; Corresponding author.State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, ChinaKey Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, College of Resources and Environment, Jilin Agricultural University, Changchun 130118, ChinaIn this study, we exposed adult male crayfish (Procambarus clarkii) to different concentrations of diclofenac (DCF) for 96 h. In the meantime, we investigated the alternations of hepatopancreatic pathology, molecular regulation and intestinal microbiota of P. clarkii exposed to DCF. The results demonstrated DCF led to histological changes including epithelium vacuolization and tubule lumen dilatation in the hepatopancreas. Transcriptome sequencing analysis showed that 642 and 586 genes were differentially expressed in the hepatopancreas of P. clarkii exposed to 1 and 10 mg/L DCF, respectively. DCF could affect the functions of antioxidation, immunity and metabolism of hepatopancreas by inducing the abnormal expressions of immune- and redox-related genes. GO enrichment results demonstrated that 10 mg/L DCF exposure could modulate the processes of molting, amino sugar metabolism, protein hydrolysis and intracellular protein translocation of P. clarkii. Additionally, the abundances of bacterial families including Shewanellaceae, Bacteroidaceae, Vibrionaceae, Erysipelotrichaceae, Aeromonadaceae, Moraxellaceae, etc. in the intestine were significantly changed after DCF exposure, and the disruption of intestinal flora might further cause abnormal intestinal metabolism in P. clarkii. This study provides novel mechanistic insights into the toxic effects of anti-inflammatory drugs on aquatic crustaceans.http://www.sciencedirect.com/science/article/pii/S0147651322009083DiclofenacHepatopancreatic histologyMolecular regulationIntestinal microbiotaProcambarus clarkii |
| spellingShingle | Yu Zhang Zheyu Li Yanxiang Zhang Kai Sun Nanqi Ren Mingtang Li Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiota Ecotoxicology and Environmental Safety Diclofenac Hepatopancreatic histology Molecular regulation Intestinal microbiota Procambarus clarkii |
| title | Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiota |
| title_full | Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiota |
| title_fullStr | Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiota |
| title_full_unstemmed | Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiota |
| title_short | Acute toxic effects of diclofenac exposure on freshwater crayfish (Procambarus clarkii): Insights from hepatopancreatic pathology, molecular regulation and intestinal microbiota |
| title_sort | acute toxic effects of diclofenac exposure on freshwater crayfish procambarus clarkii insights from hepatopancreatic pathology molecular regulation and intestinal microbiota |
| topic | Diclofenac Hepatopancreatic histology Molecular regulation Intestinal microbiota Procambarus clarkii |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322009083 |
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