Ethosomes and Transethosomes for Mangiferin Transdermal Delivery
Mangiferin is a natural glucosyl xanthone with antioxidant and anti-inflammatory activity, making it suitable for protection against cutaneous diseases. In this study ethosomes and transethosomes were designed as topical delivery systems for mangiferin. A preformulation study was conducted using dif...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/5/768 |
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| author | Maddalena Sguizzato Francesca Ferrara Supandeep Singh Hallan Anna Baldisserotto Markus Drechsler Manuela Malatesta Manuela Costanzo Rita Cortesi Carmelo Puglia Giuseppe Valacchi Elisabetta Esposito |
| author_facet | Maddalena Sguizzato Francesca Ferrara Supandeep Singh Hallan Anna Baldisserotto Markus Drechsler Manuela Malatesta Manuela Costanzo Rita Cortesi Carmelo Puglia Giuseppe Valacchi Elisabetta Esposito |
| author_sort | Maddalena Sguizzato |
| collection | DOAJ |
| description | Mangiferin is a natural glucosyl xanthone with antioxidant and anti-inflammatory activity, making it suitable for protection against cutaneous diseases. In this study ethosomes and transethosomes were designed as topical delivery systems for mangiferin. A preformulation study was conducted using different surfactants in association with phosphatidylcholine. Vesicle dimensional distribution was monitored by photon correlation spectroscopy, while antioxidant capacity and cytotoxicity were respectively assessed by free radical scavenging analysis and MTT on HaCaT keratinocytes. Selected nanosystems were further investigated by cryogenic transmission electron microscopy, while mangiferin entrapment capacity was evaluated by ultracentrifugation and HPLC. The diffusion kinetics of mangiferin from ethosomes and transethosomes evaluated by Franz cell was faster in the case of transethosomes. The suitability of mangiferin-containing nanovesicles in the treatment of skin disorders related to pollutants was investigated, evaluating, in vitro, the antioxidant and anti-inflammatory effect of ethosomes and transethosomes on human keratinocytes exposed to cigarette smoke as an oxidative and inflammatory challenger. The ability to induce an antioxidant response (HO-1) and anti-inflammatory status (IL-6 and NF-kB) was determined by RT-PCR and immunofluorescence. The data demonstrated the effectiveness of mangiferin loaded in nanosystems to protect cells from damage. Finally, to gain insight into the keratinocytes’ uptake of ethosome and transethosome, transmission electron microscopy analyses were conducted, showing that both nanosystems were able to pass intact within the cells. |
| first_indexed | 2024-03-10T11:30:15Z |
| format | Article |
| id | doaj.art-f7ae394bda3b4fb4888943d1a60f2e33 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:30:15Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f7ae394bda3b4fb4888943d1a60f2e332023-11-21T19:21:15ZengMDPI AGAntioxidants2076-39212021-05-0110576810.3390/antiox10050768Ethosomes and Transethosomes for Mangiferin Transdermal DeliveryMaddalena Sguizzato0Francesca Ferrara1Supandeep Singh Hallan2Anna Baldisserotto3Markus Drechsler4Manuela Malatesta5Manuela Costanzo6Rita Cortesi7Carmelo Puglia8Giuseppe Valacchi9Elisabetta Esposito10Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, I-44121 Ferrara, ItalyDepartment of Neurosciences and Rehabilitation, University of Ferrara, I-44121 Ferrara, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, I-44121 Ferrara, ItalyDepartment of Life Sciences and Biotechnology, University of Ferrara, I-44121 Ferrara, ItalyBavarian Polymer Institute (BPI) Keylab “Electron and Optical Microscopy” University of Bayreuth, D-95440 Bayreuth, GermanyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, I-44121 Ferrara, ItalyDepartment of Drug and Health Sciences, University of Catania, Viale Andrea Doria 6, I-95125 Catania, ItalyDepartment of Neurosciences and Rehabilitation, University of Ferrara, I-44121 Ferrara, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, I-44121 Ferrara, ItalyMangiferin is a natural glucosyl xanthone with antioxidant and anti-inflammatory activity, making it suitable for protection against cutaneous diseases. In this study ethosomes and transethosomes were designed as topical delivery systems for mangiferin. A preformulation study was conducted using different surfactants in association with phosphatidylcholine. Vesicle dimensional distribution was monitored by photon correlation spectroscopy, while antioxidant capacity and cytotoxicity were respectively assessed by free radical scavenging analysis and MTT on HaCaT keratinocytes. Selected nanosystems were further investigated by cryogenic transmission electron microscopy, while mangiferin entrapment capacity was evaluated by ultracentrifugation and HPLC. The diffusion kinetics of mangiferin from ethosomes and transethosomes evaluated by Franz cell was faster in the case of transethosomes. The suitability of mangiferin-containing nanovesicles in the treatment of skin disorders related to pollutants was investigated, evaluating, in vitro, the antioxidant and anti-inflammatory effect of ethosomes and transethosomes on human keratinocytes exposed to cigarette smoke as an oxidative and inflammatory challenger. The ability to induce an antioxidant response (HO-1) and anti-inflammatory status (IL-6 and NF-kB) was determined by RT-PCR and immunofluorescence. The data demonstrated the effectiveness of mangiferin loaded in nanosystems to protect cells from damage. Finally, to gain insight into the keratinocytes’ uptake of ethosome and transethosome, transmission electron microscopy analyses were conducted, showing that both nanosystems were able to pass intact within the cells.https://www.mdpi.com/2076-3921/10/5/768ethosomestransethosomesmangiferinfranz cellantioxidants |
| spellingShingle | Maddalena Sguizzato Francesca Ferrara Supandeep Singh Hallan Anna Baldisserotto Markus Drechsler Manuela Malatesta Manuela Costanzo Rita Cortesi Carmelo Puglia Giuseppe Valacchi Elisabetta Esposito Ethosomes and Transethosomes for Mangiferin Transdermal Delivery Antioxidants ethosomes transethosomes mangiferin franz cell antioxidants |
| title | Ethosomes and Transethosomes for Mangiferin Transdermal Delivery |
| title_full | Ethosomes and Transethosomes for Mangiferin Transdermal Delivery |
| title_fullStr | Ethosomes and Transethosomes for Mangiferin Transdermal Delivery |
| title_full_unstemmed | Ethosomes and Transethosomes for Mangiferin Transdermal Delivery |
| title_short | Ethosomes and Transethosomes for Mangiferin Transdermal Delivery |
| title_sort | ethosomes and transethosomes for mangiferin transdermal delivery |
| topic | ethosomes transethosomes mangiferin franz cell antioxidants |
| url | https://www.mdpi.com/2076-3921/10/5/768 |
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