Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa
In bilaterians and cnidarians, epithelial cell-polarity is regulated by the interactions between Par proteins, Wnt/PCP signaling pathway, and cell-cell adhesion. Par proteins are highly conserved across Metazoa, including ctenophores. But strikingly, ctenophore genomes lack components of the Wnt/PCP...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/54927 |
| _version_ | 1811253039183429632 |
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| author | Miguel Salinas-Saavedra Mark Q Martindale |
| author_facet | Miguel Salinas-Saavedra Mark Q Martindale |
| author_sort | Miguel Salinas-Saavedra |
| collection | DOAJ |
| description | In bilaterians and cnidarians, epithelial cell-polarity is regulated by the interactions between Par proteins, Wnt/PCP signaling pathway, and cell-cell adhesion. Par proteins are highly conserved across Metazoa, including ctenophores. But strikingly, ctenophore genomes lack components of the Wnt/PCP pathway and cell-cell adhesion complexes raising the question if ctenophore cells are polarized by mechanisms involving Par proteins. Here, by using immunohistochemistry and live-cell imaging of specific mRNAs, we describe for the first time the subcellular localization of selected Par proteins in blastomeres and epithelial cells during the embryogenesis of the ctenophore Mnemiopsis leidyi. We show that these proteins distribute differently compared to what has been described for other animals, even though they segregate in a host-specific fashion when expressed in cnidarian embryos. This differential localization might be related to the emergence of different junctional complexes during metazoan evolution. |
| first_indexed | 2024-04-12T16:44:45Z |
| format | Article |
| id | doaj.art-f7afbc1f13de421295e8cf7c66d77e60 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:44:45Z |
| publishDate | 2020-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-f7afbc1f13de421295e8cf7c66d77e602022-12-22T03:24:39ZengeLife Sciences Publications LtdeLife2050-084X2020-07-01910.7554/eLife.54927Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoaMiguel Salinas-Saavedra0https://orcid.org/0000-0002-1598-9881Mark Q Martindale1The Whitney Laboratory for Marine Bioscience, and the Department of Biology, University of Florida, St. Augustine, United StatesThe Whitney Laboratory for Marine Bioscience, and the Department of Biology, University of Florida, St. Augustine, United StatesIn bilaterians and cnidarians, epithelial cell-polarity is regulated by the interactions between Par proteins, Wnt/PCP signaling pathway, and cell-cell adhesion. Par proteins are highly conserved across Metazoa, including ctenophores. But strikingly, ctenophore genomes lack components of the Wnt/PCP pathway and cell-cell adhesion complexes raising the question if ctenophore cells are polarized by mechanisms involving Par proteins. Here, by using immunohistochemistry and live-cell imaging of specific mRNAs, we describe for the first time the subcellular localization of selected Par proteins in blastomeres and epithelial cells during the embryogenesis of the ctenophore Mnemiopsis leidyi. We show that these proteins distribute differently compared to what has been described for other animals, even though they segregate in a host-specific fashion when expressed in cnidarian embryos. This differential localization might be related to the emergence of different junctional complexes during metazoan evolution.https://elifesciences.org/articles/54927Mnemiopsis leidyicell polarityepitheliumctenophore |
| spellingShingle | Miguel Salinas-Saavedra Mark Q Martindale Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa eLife Mnemiopsis leidyi cell polarity epithelium ctenophore |
| title | Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa |
| title_full | Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa |
| title_fullStr | Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa |
| title_full_unstemmed | Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa |
| title_short | Par protein localization during the early development of Mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa |
| title_sort | par protein localization during the early development of mnemiopsis leidyi suggests different modes of epithelial organization in the metazoa |
| topic | Mnemiopsis leidyi cell polarity epithelium ctenophore |
| url | https://elifesciences.org/articles/54927 |
| work_keys_str_mv | AT miguelsalinassaavedra parproteinlocalizationduringtheearlydevelopmentofmnemiopsisleidyisuggestsdifferentmodesofepithelialorganizationinthemetazoa AT markqmartindale parproteinlocalizationduringtheearlydevelopmentofmnemiopsisleidyisuggestsdifferentmodesofepithelialorganizationinthemetazoa |