Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits
Objective: As one of the most valuable genetic resources of Ongole beef cattle globally, the Sumba Ongole (SO) cattle population is being studied in this investigation of myostatin (MSTN) gene polymorphism and its association with growth traits. Materials and Methods: Blood samples from 161 SO catt...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2022-12-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=108724 |
| _version_ | 1797957201063575552 |
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| author | Cynthia Dewi Gaina Filphin Adolfin Amalo |
| author_facet | Cynthia Dewi Gaina Filphin Adolfin Amalo |
| author_sort | Cynthia Dewi Gaina |
| collection | DOAJ |
| description | Objective: As one of the most valuable genetic resources of Ongole beef cattle globally, the Sumba Ongole (SO) cattle population is being studied in this investigation of myostatin (MSTN) gene polymorphism and its association with growth traits.
Materials and Methods: Blood samples from 161 SO cattle were collected and analyzed. Deoxyribonucleic acid (DNA) was isolated. The DNA was electrophoresed and extracted, and finally, the annealing temperature was optimized, followed by amplification and sequencing. Next, we used a Basic local alignment search tool to assess the sequencing data.
Results: The analysis revealed 22 single nucleotide polymorphisms (SNPs) in the MSTN gene in this region that showed genetic variation. Two SNPs, c.424 G > A, and c.467 G > C, were found to be significantly associated with SO cattle phenotypes of wither height, heart girth, and hip height (p < 0.05) but not with body weight or body length (p > 0.05).
Conclusion: As a result of our findings, the MSTN gene polymorphism and its correlation with growth traits in SO cattle may be employed as a candidate marker in SO cattle and other beef cattle breeds. [J Adv Vet Anim Res 2022; 9(4.000): 565-572] |
| first_indexed | 2024-04-11T00:01:43Z |
| format | Article |
| id | doaj.art-f7b1d956f37849969e420480b27f1597 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-04-11T00:01:43Z |
| publishDate | 2022-12-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-f7b1d956f37849969e420480b27f15972023-01-10T04:13:29ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102022-12-019456557210.5455/javar.2022.i625108724Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traitsCynthia Dewi Gaina0Filphin Adolfin Amalo1Laboratory of Veterinary Clinic, Reproduction, Pathology, and Nutrition, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia Laboratory of Veterinary Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia.Objective: As one of the most valuable genetic resources of Ongole beef cattle globally, the Sumba Ongole (SO) cattle population is being studied in this investigation of myostatin (MSTN) gene polymorphism and its association with growth traits. Materials and Methods: Blood samples from 161 SO cattle were collected and analyzed. Deoxyribonucleic acid (DNA) was isolated. The DNA was electrophoresed and extracted, and finally, the annealing temperature was optimized, followed by amplification and sequencing. Next, we used a Basic local alignment search tool to assess the sequencing data. Results: The analysis revealed 22 single nucleotide polymorphisms (SNPs) in the MSTN gene in this region that showed genetic variation. Two SNPs, c.424 G > A, and c.467 G > C, were found to be significantly associated with SO cattle phenotypes of wither height, heart girth, and hip height (p < 0.05) but not with body weight or body length (p > 0.05). Conclusion: As a result of our findings, the MSTN gene polymorphism and its correlation with growth traits in SO cattle may be employed as a candidate marker in SO cattle and other beef cattle breeds. [J Adv Vet Anim Res 2022; 9(4.000): 565-572]http://www.ejmanager.com/fulltextpdf.php?mno=108724myostatin gene; polymorphism; sumba ongole cattle; bos indicus; growth traits |
| spellingShingle | Cynthia Dewi Gaina Filphin Adolfin Amalo Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits Journal of Advanced Veterinary and Animal Research myostatin gene; polymorphism; sumba ongole cattle; bos indicus; growth traits |
| title | Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits |
| title_full | Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits |
| title_fullStr | Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits |
| title_full_unstemmed | Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits |
| title_short | Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits |
| title_sort | genetic polymorphism of myostatin gene in sumba ongole bos indicus cattle and its association with growth traits |
| topic | myostatin gene; polymorphism; sumba ongole cattle; bos indicus; growth traits |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=108724 |
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