Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients
Abstract Patients worldwide require therapeutic transfusions of packed red blood cells (pRBCs), which is applied to the high-risk patients who need periodic transfusions due to leukemia, lymphoma, myeloma and other blood diseases or disorders. Contrary to the general hospital population where the tr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26309-5 |
| _version_ | 1797980192337035264 |
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| author | Jakub Dybas Aleksandra Wajda Fatih Celal Alcicek Magdalena Kaczmarska Katarzyna Bulat Ewa Szczesny-Malysiak Agnieszka Martyna David Perez-Guaita Tomasz Sacha Katarzyna M. Marzec |
| author_facet | Jakub Dybas Aleksandra Wajda Fatih Celal Alcicek Magdalena Kaczmarska Katarzyna Bulat Ewa Szczesny-Malysiak Agnieszka Martyna David Perez-Guaita Tomasz Sacha Katarzyna M. Marzec |
| author_sort | Jakub Dybas |
| collection | DOAJ |
| description | Abstract Patients worldwide require therapeutic transfusions of packed red blood cells (pRBCs), which is applied to the high-risk patients who need periodic transfusions due to leukemia, lymphoma, myeloma and other blood diseases or disorders. Contrary to the general hospital population where the transfusions are carried out mainly for healthy trauma patients, in case of high-risk patients the proper quality of pRBCs is crucial. This leads to an increased demand for efficient technology providing information on the pRBCs alterations deteriorating their quality. Here we present the design of an innovative, label-free, noninvasive, rapid Raman spectroscopy-based method for pRBCs quality evaluation, starting with the description of sample measurement and data analysis, through correlation of spectroscopic results with reference techniques' outcomes, and finishing with methodology verification and its application in clinical conditions. We have shown that Raman spectra collected from the pRBCs supernatant mixture with a proper chemometric analysis conducted for a minimum one ratio of integral intensities of the chosen Raman marker bands within the spectrum allow evaluation of the pRBC quality in a rapid, noninvasive, and free-label manner, without unsealing the pRBCs bag. Subsequently, spectroscopic data were compared with predefined reference values, either from pRBCs expiration or those defining the pRBCs quality, allowing to assess their utility for transfusion to patients with acute myeloid leukemia (AML) and lymphoblastic leukemia (ALL). |
| first_indexed | 2024-04-11T05:50:34Z |
| format | Article |
| id | doaj.art-f7b3d69bd3924a88b11fff23ae3d8ab5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:50:34Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f7b3d69bd3924a88b11fff23ae3d8ab52022-12-22T04:42:05ZengNature PortfolioScientific Reports2045-23222022-12-0112111210.1038/s41598-022-26309-5Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patientsJakub Dybas0Aleksandra Wajda1Fatih Celal Alcicek2Magdalena Kaczmarska3Katarzyna Bulat4Ewa Szczesny-Malysiak5Agnieszka Martyna6David Perez-Guaita7Tomasz Sacha8Katarzyna M. Marzec9Jagiellonian Center for Experimental Therapeutics, Jagiellonian UniversityFaculty of Chemistry, Jagiellonian UniversityJagiellonian Center for Experimental Therapeutics, Jagiellonian UniversityJagiellonian Center for Experimental Therapeutics, Jagiellonian UniversityJagiellonian Center for Experimental Therapeutics, Jagiellonian UniversityJagiellonian Center for Experimental Therapeutics, Jagiellonian UniversityForensic Chemistry Research Group, University of Silesia in KatowiceDepartment of Analytical Chemistry, University of ValanciaChair of Haematology, Faculty of Medicine, Jagiellonian University Medical CollegeJagiellonian Center for Experimental Therapeutics, Jagiellonian UniversityAbstract Patients worldwide require therapeutic transfusions of packed red blood cells (pRBCs), which is applied to the high-risk patients who need periodic transfusions due to leukemia, lymphoma, myeloma and other blood diseases or disorders. Contrary to the general hospital population where the transfusions are carried out mainly for healthy trauma patients, in case of high-risk patients the proper quality of pRBCs is crucial. This leads to an increased demand for efficient technology providing information on the pRBCs alterations deteriorating their quality. Here we present the design of an innovative, label-free, noninvasive, rapid Raman spectroscopy-based method for pRBCs quality evaluation, starting with the description of sample measurement and data analysis, through correlation of spectroscopic results with reference techniques' outcomes, and finishing with methodology verification and its application in clinical conditions. We have shown that Raman spectra collected from the pRBCs supernatant mixture with a proper chemometric analysis conducted for a minimum one ratio of integral intensities of the chosen Raman marker bands within the spectrum allow evaluation of the pRBC quality in a rapid, noninvasive, and free-label manner, without unsealing the pRBCs bag. Subsequently, spectroscopic data were compared with predefined reference values, either from pRBCs expiration or those defining the pRBCs quality, allowing to assess their utility for transfusion to patients with acute myeloid leukemia (AML) and lymphoblastic leukemia (ALL).https://doi.org/10.1038/s41598-022-26309-5 |
| spellingShingle | Jakub Dybas Aleksandra Wajda Fatih Celal Alcicek Magdalena Kaczmarska Katarzyna Bulat Ewa Szczesny-Malysiak Agnieszka Martyna David Perez-Guaita Tomasz Sacha Katarzyna M. Marzec Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients Scientific Reports |
| title | Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients |
| title_full | Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients |
| title_fullStr | Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients |
| title_full_unstemmed | Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients |
| title_short | Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients |
| title_sort | label free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients |
| url | https://doi.org/10.1038/s41598-022-26309-5 |
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