Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex
Natural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree, <i>Hevea brasiliensis</i>. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/1422-0067/21/15/5282 |
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| author | Li Yu Boxuan Yuan Lingling Wang Yong Sun Guohua Ding Ousmane Ahmat Souleymane Xueyan Zhang Quanliang Xie Xuchu Wang |
| author_facet | Li Yu Boxuan Yuan Lingling Wang Yong Sun Guohua Ding Ousmane Ahmat Souleymane Xueyan Zhang Quanliang Xie Xuchu Wang |
| author_sort | Li Yu |
| collection | DOAJ |
| description | Natural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree, <i>Hevea brasiliensis</i>. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications (PTMs) of latex proteins, such as phosphorylation, glycosylation and ubiquitination, are crucial in natural rubber biosynthesis. In this study, comparative proteomics was performed to identify the glycosylated proteins in rubber latex treated with ethylene for different days. Combined with Pro-Q Glycoprotein gel staining and mass spectrometry techniques, we provided the first visual profiling of glycoproteomics of rubber latex and finally identified 144 glycosylated protein species, including 65 differentially accumulated proteins (DAPs) after treating with ethylene for three and/or five days. Gene Ontology (GO) functional annotation showed that these ethylene-responsive glycoproteins are mainly involved in cell parts, membrane components and metabolism. Pathway analysis demonstrated that these glycosylated rubber latex proteins are mainly involved in carbohydrate metabolism, energy metabolism, degradation function and cellular processes in rubber latex metabolism. Protein–protein interaction analysis revealed that these DAPs are mainly centered on acetyl-CoA acetyltransferase and hydroxymethylglutaryl-CoA synthase (HMGS) in the mevalonate pathway for natural rubber biosynthesis. In our glycoproteomics, three protein isoforms of HMGS2 were identified from rubber latex, and only one HMGS2 isoform was sharply increased in rubber latex by ethylene treatment for five days. Furthermore, the <i>HbHMGS2</i> gene was over-expressed in a model rubber-producing grass <i>Taraxacum Kok-saghyz</i> and rubber content in the roots of transgenic rubber grass was significantly increased over that in the wild type plant, indicating HMGS2 is the key component for natural rubber production. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f7b402e36b4c45d19220f6165af3c9212023-11-20T07:56:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012115528210.3390/ijms21155282Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber LatexLi Yu0Boxuan Yuan1Lingling Wang2Yong Sun3Guohua Ding4Ousmane Ahmat Souleymane5Xueyan Zhang6Quanliang Xie7Xuchu Wang8College of Life Sciences, Shihezi University, Shihezi 832003, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, ChinaKey Laboratory for Ecology of Tropical Islands, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou 571158, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, ChinaKey Laboratory for Ecology of Tropical Islands, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou 571158, ChinaKey Laboratory for Ecology of Tropical Islands, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou 571158, ChinaKey Laboratory for Ecology of Tropical Islands, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou 571158, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832003, ChinaNatural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree, <i>Hevea brasiliensis</i>. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications (PTMs) of latex proteins, such as phosphorylation, glycosylation and ubiquitination, are crucial in natural rubber biosynthesis. In this study, comparative proteomics was performed to identify the glycosylated proteins in rubber latex treated with ethylene for different days. Combined with Pro-Q Glycoprotein gel staining and mass spectrometry techniques, we provided the first visual profiling of glycoproteomics of rubber latex and finally identified 144 glycosylated protein species, including 65 differentially accumulated proteins (DAPs) after treating with ethylene for three and/or five days. Gene Ontology (GO) functional annotation showed that these ethylene-responsive glycoproteins are mainly involved in cell parts, membrane components and metabolism. Pathway analysis demonstrated that these glycosylated rubber latex proteins are mainly involved in carbohydrate metabolism, energy metabolism, degradation function and cellular processes in rubber latex metabolism. Protein–protein interaction analysis revealed that these DAPs are mainly centered on acetyl-CoA acetyltransferase and hydroxymethylglutaryl-CoA synthase (HMGS) in the mevalonate pathway for natural rubber biosynthesis. In our glycoproteomics, three protein isoforms of HMGS2 were identified from rubber latex, and only one HMGS2 isoform was sharply increased in rubber latex by ethylene treatment for five days. Furthermore, the <i>HbHMGS2</i> gene was over-expressed in a model rubber-producing grass <i>Taraxacum Kok-saghyz</i> and rubber content in the roots of transgenic rubber grass was significantly increased over that in the wild type plant, indicating HMGS2 is the key component for natural rubber production.https://www.mdpi.com/1422-0067/21/15/5282comparative proteomics<i>Hevea brasiliensis</i>Hydroxymethylglutaryl-CoA synthaseglycosylated proteinsnatural rubber biosynthesisrubber latex |
| spellingShingle | Li Yu Boxuan Yuan Lingling Wang Yong Sun Guohua Ding Ousmane Ahmat Souleymane Xueyan Zhang Quanliang Xie Xuchu Wang Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex International Journal of Molecular Sciences comparative proteomics <i>Hevea brasiliensis</i> Hydroxymethylglutaryl-CoA synthase glycosylated proteins natural rubber biosynthesis rubber latex |
| title | Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex |
| title_full | Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex |
| title_fullStr | Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex |
| title_full_unstemmed | Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex |
| title_short | Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex |
| title_sort | identification and characterization of glycoproteins and their responsive patterns upon ethylene stimulation in the rubber latex |
| topic | comparative proteomics <i>Hevea brasiliensis</i> Hydroxymethylglutaryl-CoA synthase glycosylated proteins natural rubber biosynthesis rubber latex |
| url | https://www.mdpi.com/1422-0067/21/15/5282 |
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