Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume)
Tuber rot has been reported as one of the limiting factors to porang (Amorphophallus muelleri Blume) tuber production. Bacterial infection is one of the main causes of tuber rot during storage. In this study, a total of seven bacterial isolates obtained from porang tubers were tested for their poten...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2020-04-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1238 |
| _version_ | 1797425236881178624 |
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| author | Afifah Nur Aini Rodiyati Azrianingsih Irfan Mustafa |
| author_facet | Afifah Nur Aini Rodiyati Azrianingsih Irfan Mustafa |
| author_sort | Afifah Nur Aini |
| collection | DOAJ |
| description | Tuber rot has been reported as one of the limiting factors to porang (Amorphophallus muelleri Blume) tuber production. Bacterial infection is one of the main causes of tuber rot during storage. In this study, a total of seven bacterial isolates obtained from porang tubers were tested for their potency to cause rotting on porang tubers. Porang tubers were immersed in bacterial suspension and incubated for 14 days. The physical properties of the tubers such as texture and color, and the chemical properties such as reducing sugar and ethanol content were determined at the end of incubation period. All infected tubers were soft, slimy and darker. Two isolates, T4 and T9A, had the highest potency to cause tuber rot indicated by the significantly higher reducing sugar content (14.38 and 15.32 mg/ml) and higher ethanol content (32.17 and 35.05%) compared to control tubers which were not infected with bacteria. Both isolates were identified as Bacillus altitudinis and Pseudomonas stutzeri, respectively, based on 16S rDNA sequence analysis. |
| first_indexed | 2024-03-09T08:13:07Z |
| format | Article |
| id | doaj.art-f7b6a5774fe84c12a4bea68497885468 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-09T08:13:07Z |
| publishDate | 2020-04-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-f7b6a5774fe84c12a4bea684978854682023-12-02T23:07:06ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172020-04-011029910410.11594/jtls.10.02.02332Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume)Afifah Nur Aini0Rodiyati Azrianingsih1Irfan Mustafa2Department of Biology, Faculty of Mathematics and Natural Sciences, University of BrawijayaDepartment of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya Porang Research Centre, University of BrawijayaDepartment of Biology, Faculty of Mathematics and Natural Sciences, University of BrawijayaTuber rot has been reported as one of the limiting factors to porang (Amorphophallus muelleri Blume) tuber production. Bacterial infection is one of the main causes of tuber rot during storage. In this study, a total of seven bacterial isolates obtained from porang tubers were tested for their potency to cause rotting on porang tubers. Porang tubers were immersed in bacterial suspension and incubated for 14 days. The physical properties of the tubers such as texture and color, and the chemical properties such as reducing sugar and ethanol content were determined at the end of incubation period. All infected tubers were soft, slimy and darker. Two isolates, T4 and T9A, had the highest potency to cause tuber rot indicated by the significantly higher reducing sugar content (14.38 and 15.32 mg/ml) and higher ethanol content (32.17 and 35.05%) compared to control tubers which were not infected with bacteria. Both isolates were identified as Bacillus altitudinis and Pseudomonas stutzeri, respectively, based on 16S rDNA sequence analysis.https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1238amorphophallus muelleripathogen bacteriatuber rotporang |
| spellingShingle | Afifah Nur Aini Rodiyati Azrianingsih Irfan Mustafa Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) Journal of Tropical Life Science amorphophallus muelleri pathogen bacteria tuber rot porang |
| title | Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) |
| title_full | Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) |
| title_fullStr | Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) |
| title_full_unstemmed | Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) |
| title_short | Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) |
| title_sort | identification of potential pathogen bacteria causing tuber rot in porang amorphophallus muelleri blume |
| topic | amorphophallus muelleri pathogen bacteria tuber rot porang |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1238 |
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