Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite production

Hevea brasiliensis was introduced to Thailand for the production of natural rubber. RRIT251 and RRIM600 are high yield varieties while PB350 produces a high yield of latex and resistance to diseases. The cultivar identity must be ascertained prior to plantation establishment because it is importan...

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Main Authors: Rugchanok Puwaphut, Alisa Nakkaew, Amornrat Phongdara
Format: Article
Language:English
Published: Prince of Songkla University 2016-02-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://rdo.psu.ac.th/sjstweb/journal/38-1/38-1-3.pdf
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author Rugchanok Puwaphut
Alisa Nakkaew
Amornrat Phongdara
author_facet Rugchanok Puwaphut
Alisa Nakkaew
Amornrat Phongdara
author_sort Rugchanok Puwaphut
collection DOAJ
description Hevea brasiliensis was introduced to Thailand for the production of natural rubber. RRIT251 and RRIM600 are high yield varieties while PB350 produces a high yield of latex and resistance to diseases. The cultivar identity must be ascertained prior to plantation establishment because it is important to relate the genetic background with the production of certain secondary metabolites. In this study, the internal transcribed spacer region was used to evaluate the varieties among these 3 cultivars. Callus induction was initiated with 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 2 mg/L 6-benzylaminopurine (BAP). Hexadecanoic acid, methyl ester and 9-octadecanoic acid (Z), methyl ester were detected in RRIM600 calli. 2-Furancarboxaldehyde-5-(hydroxymethyl) was dominant in the RRIT251 and PB350 calli. These secondary metabolites are relevant in various high performance commercial sectors.
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spelling doaj.art-f7b7ddae3c8c46a3b0074525780e26c22022-12-22T03:53:03ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952016-02-013811522Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite productionRugchanok Puwaphut0Alisa Nakkaew1Amornrat Phongdara2Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110 Thailand.Center for Genomics and Bioinformatics Research, Prince of Songkla University, Hat Yai, Songkhla, 90110 Thailand.Center for Genomics and Bioinformatics Research, Prince of Songkla University, Hat Yai, Songkhla, 90110 Thailand.Hevea brasiliensis was introduced to Thailand for the production of natural rubber. RRIT251 and RRIM600 are high yield varieties while PB350 produces a high yield of latex and resistance to diseases. The cultivar identity must be ascertained prior to plantation establishment because it is important to relate the genetic background with the production of certain secondary metabolites. In this study, the internal transcribed spacer region was used to evaluate the varieties among these 3 cultivars. Callus induction was initiated with 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 2 mg/L 6-benzylaminopurine (BAP). Hexadecanoic acid, methyl ester and 9-octadecanoic acid (Z), methyl ester were detected in RRIM600 calli. 2-Furancarboxaldehyde-5-(hydroxymethyl) was dominant in the RRIT251 and PB350 calli. These secondary metabolites are relevant in various high performance commercial sectors.http://rdo.psu.ac.th/sjstweb/journal/38-1/38-1-3.pdfHevea brasiliensisinternal transcribed spacer (ITS)9-octadecanoic acid (Z)methyl esterhexadecanoic acid2-furancarboxaldehyde-5-(hydroxymethyl)
spellingShingle Rugchanok Puwaphut
Alisa Nakkaew
Amornrat Phongdara
Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite production
Songklanakarin Journal of Science and Technology (SJST)
Hevea brasiliensis
internal transcribed spacer (ITS)
9-octadecanoic acid (Z)
methyl ester
hexadecanoic acid
2-furancarboxaldehyde-5-(hydroxymethyl)
title Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite production
title_full Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite production
title_fullStr Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite production
title_full_unstemmed Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite production
title_short Diversity among 3 cultivars (RRIM600, RRIT251, and PB350) of Hevea brasiliensis and secondary metabolite production
title_sort diversity among 3 cultivars rrim600 rrit251 and pb350 of hevea brasiliensis and secondary metabolite production
topic Hevea brasiliensis
internal transcribed spacer (ITS)
9-octadecanoic acid (Z)
methyl ester
hexadecanoic acid
2-furancarboxaldehyde-5-(hydroxymethyl)
url http://rdo.psu.ac.th/sjstweb/journal/38-1/38-1-3.pdf
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