The role of CXCR3 and its ligands expression in Brucellar spondylitis
Abstract Aim Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 an...
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| Format: | Article |
| Language: | English |
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BMC
2020-11-01
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| Series: | BMC Immunology |
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| Online Access: | http://link.springer.com/article/10.1186/s12865-020-00390-9 |
| _version_ | 1829478136542134272 |
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| author | Xin Hu Xiaoqian Shang Liang Wang Jiahui Fan Yue Wang Jie Lv Shaxika Nazierhan Hao Wang Jing Wang Xiumin Ma |
| author_facet | Xin Hu Xiaoqian Shang Liang Wang Jiahui Fan Yue Wang Jie Lv Shaxika Nazierhan Hao Wang Jing Wang Xiumin Ma |
| author_sort | Xin Hu |
| collection | DOAJ |
| description | Abstract Aim Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS. Methods A total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-γ, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-γ, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS. Results In lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-γ, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-γ, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls. Conclusion In our research, the expression levels of IFN-γ, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-γ, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS. |
| first_indexed | 2024-12-14T17:09:06Z |
| format | Article |
| id | doaj.art-f7b8dac81a4448f1a4297d35f5155f35 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-14T17:09:06Z |
| publishDate | 2020-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-f7b8dac81a4448f1a4297d35f5155f352022-12-21T22:53:39ZengBMCBMC Immunology1471-21722020-11-012111810.1186/s12865-020-00390-9The role of CXCR3 and its ligands expression in Brucellar spondylitisXin Hu0Xiaoqian Shang1Liang Wang2Jiahui Fan3Yue Wang4Jie Lv5Shaxika Nazierhan6Hao Wang7Jing Wang8Xiumin Ma9State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityDepartment of Spinal Surgery, People’s Hospital of Xinjiang Uygur Autonomous RegionDepartment of Spinal Surgery, People’s Hospital of Xinjiang Uygur Autonomous RegionState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityState Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical UniversityAbstract Aim Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS. Methods A total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-γ, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-γ, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS. Results In lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-γ, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-γ, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls. Conclusion In our research, the expression levels of IFN-γ, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-γ, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS.http://link.springer.com/article/10.1186/s12865-020-00390-9Brucellar spondylitisIFN-γCXCR3CXCL9CXCL10 |
| spellingShingle | Xin Hu Xiaoqian Shang Liang Wang Jiahui Fan Yue Wang Jie Lv Shaxika Nazierhan Hao Wang Jing Wang Xiumin Ma The role of CXCR3 and its ligands expression in Brucellar spondylitis BMC Immunology Brucellar spondylitis IFN-γ CXCR3 CXCL9 CXCL10 |
| title | The role of CXCR3 and its ligands expression in Brucellar spondylitis |
| title_full | The role of CXCR3 and its ligands expression in Brucellar spondylitis |
| title_fullStr | The role of CXCR3 and its ligands expression in Brucellar spondylitis |
| title_full_unstemmed | The role of CXCR3 and its ligands expression in Brucellar spondylitis |
| title_short | The role of CXCR3 and its ligands expression in Brucellar spondylitis |
| title_sort | role of cxcr3 and its ligands expression in brucellar spondylitis |
| topic | Brucellar spondylitis IFN-γ CXCR3 CXCL9 CXCL10 |
| url | http://link.springer.com/article/10.1186/s12865-020-00390-9 |
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