Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells
Summary: Human umbilical cord mesenchymal stem cells (UC-MSCs)-derived hepatocyte-like cells (HLCs) have shown great promise in the treatment of liver diseases. However, most current induction protocols yield hepatocyte-like cells with limited function as compared with primary hepatocytes. Schisandr...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224001330 |
| _version_ | 1797338259221643264 |
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| author | Meixian Jin Xiao Yi Xiaojuan Zhu Wei Hu Simin Wang Qi Chen Wanren Yang Yang Li Shao Li Qing Peng Mingxin Pan Yi Gao Shiyuan Xu Ying Zhang Shuqin Zhou |
| author_facet | Meixian Jin Xiao Yi Xiaojuan Zhu Wei Hu Simin Wang Qi Chen Wanren Yang Yang Li Shao Li Qing Peng Mingxin Pan Yi Gao Shiyuan Xu Ying Zhang Shuqin Zhou |
| author_sort | Meixian Jin |
| collection | DOAJ |
| description | Summary: Human umbilical cord mesenchymal stem cells (UC-MSCs)-derived hepatocyte-like cells (HLCs) have shown great promise in the treatment of liver diseases. However, most current induction protocols yield hepatocyte-like cells with limited function as compared with primary hepatocytes. Schisandrin B (Sch B) is one of the main components of Schisandra chinensis, which can prevent fibrosis progression and promote liver cell regeneration. Herein, we investigated the effects of Sch B on hepatic differentiation of UC-MSCs. We found that treatment with 10 μM Sch B from the second stage of the differentiation process increased hepatic marker levels and hepatic function. Additionally, RNA-seq analysis revealed that Sch B promoted hepatic differentiation via activating the JAK2/STAT3 pathway. When transplanted HLCs into mice with CCL4-induced liver fibrosis, Sch B-treated HLCs exhibited significant therapeutic effects. This study provides an optimized hepatic differentiation protocol for UC-MSCs based on Sch B, yielding functioning cells for liver disease treatment. |
| first_indexed | 2024-03-08T09:28:26Z |
| format | Article |
| id | doaj.art-f7bb8acacc1c4de7bde4a2e5a5f29cbb |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T09:28:26Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-f7bb8acacc1c4de7bde4a2e5a5f29cbb2024-01-31T05:45:20ZengElsevieriScience2589-00422024-02-01272108912Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cellsMeixian Jin0Xiao Yi1Xiaojuan Zhu2Wei Hu3Simin Wang4Qi Chen5Wanren Yang6Yang Li7Shao Li8Qing Peng9Mingxin Pan10Yi Gao11Shiyuan Xu12Ying Zhang13Shuqin Zhou14Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaDepartment of Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaDepartment of Anesthesiology, First People’s Hospital of Kashi, Kashi 844000, ChinaDepartment of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaDepartment of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaDepartment of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaGeneral Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaGeneral Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaGeneral Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaGeneral Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaGeneral Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaGeneral Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, ChinaDepartment of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China; Corresponding authorGeneral Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China; Corresponding authorDepartment of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510000, China; Anesthesiology Department of The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of Shenzhen, Shenzhen 518172, China; Corresponding authorSummary: Human umbilical cord mesenchymal stem cells (UC-MSCs)-derived hepatocyte-like cells (HLCs) have shown great promise in the treatment of liver diseases. However, most current induction protocols yield hepatocyte-like cells with limited function as compared with primary hepatocytes. Schisandrin B (Sch B) is one of the main components of Schisandra chinensis, which can prevent fibrosis progression and promote liver cell regeneration. Herein, we investigated the effects of Sch B on hepatic differentiation of UC-MSCs. We found that treatment with 10 μM Sch B from the second stage of the differentiation process increased hepatic marker levels and hepatic function. Additionally, RNA-seq analysis revealed that Sch B promoted hepatic differentiation via activating the JAK2/STAT3 pathway. When transplanted HLCs into mice with CCL4-induced liver fibrosis, Sch B-treated HLCs exhibited significant therapeutic effects. This study provides an optimized hepatic differentiation protocol for UC-MSCs based on Sch B, yielding functioning cells for liver disease treatment.http://www.sciencedirect.com/science/article/pii/S2589004224001330Cellular therapyStem cells researchBioactive plant product |
| spellingShingle | Meixian Jin Xiao Yi Xiaojuan Zhu Wei Hu Simin Wang Qi Chen Wanren Yang Yang Li Shao Li Qing Peng Mingxin Pan Yi Gao Shiyuan Xu Ying Zhang Shuqin Zhou Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells iScience Cellular therapy Stem cells research Bioactive plant product |
| title | Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells |
| title_full | Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells |
| title_fullStr | Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells |
| title_full_unstemmed | Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells |
| title_short | Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells |
| title_sort | schisandrin b promotes hepatic differentiation from human umbilical cord mesenchymal stem cells |
| topic | Cellular therapy Stem cells research Bioactive plant product |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224001330 |
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