Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats
The aim of the study reported herein was to evaluate the suitability of the Surface Acoustic Wave (SAW) technique as a possible diagnostic tool in benign prostatic hyperplasia (BPH). Moreover, for the first time, the BPH model was a totally physiological using naturally aged rats with spontaneous, a...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-07-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580817301723 |
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| author | Maria M. Rivera del Alamo Mireia Díaz-Lobo Silvia Busquets Joan E. Rodríguez-Gil Josep M. Fernández-Novell |
| author_facet | Maria M. Rivera del Alamo Mireia Díaz-Lobo Silvia Busquets Joan E. Rodríguez-Gil Josep M. Fernández-Novell |
| author_sort | Maria M. Rivera del Alamo |
| collection | DOAJ |
| description | The aim of the study reported herein was to evaluate the suitability of the Surface Acoustic Wave (SAW) technique as a possible diagnostic tool in benign prostatic hyperplasia (BPH). Moreover, for the first time, the BPH model was a totally physiological using naturally aged rats with spontaneous, age-related BPH instead of the pharmacologically induced models usually used. Eighteen male Wistar rats were distributed according to their age: 6 weeks (young), 12 weeks (adult) and 12 months (old) old. Prostate gland was removed and analyzed by mini-arrays, Western blotting (WB) and SAW techniques. Mini-arrays indicated that there were significant differences in the expression of 29/34 inflammation-related cytokines. WB was carried out to confirm the results after selection of 4 cytokines from which one showed no changes, namely PDGF-AA, and the other three, which significantly increase in older animals, were CD86, β-NGF and VEGF. Notwithstanding, WB of old rats yielded confusing results due to an anomalous migration of proteins, dismissing this technique as an useful tool in these animals. Accurate results in old rats were uniquely obtained by using the SAW technique. Thus, SAW analysis showed that there were not differences among groups in the amount of PDGF-AA. On the contrary, SAW analysis showed that amounts of CD86, β-NGF and VEGF in old rats were 2.0, 1.9 and 5.7-fold higher than that from young ones, respectively. These results indicate that SAW is a highly accurate technique for determining changes in the cytokines expression in BPH. |
| first_indexed | 2024-04-11T23:36:25Z |
| format | Article |
| id | doaj.art-f7bea621363f4692b828046e5fa219a7 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-11T23:36:25Z |
| publishDate | 2018-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-f7bea621363f4692b828046e5fa219a72022-12-22T03:56:57ZengElsevierBiochemistry and Biophysics Reports2405-58082018-07-0114C263410.1016/j.bbrep.2018.03.005Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in ratsMaria M. Rivera del Alamo0Mireia Díaz-Lobo1Silvia Busquets2Joan E. Rodríguez-Gil3Josep M. Fernández-Novell4Dept. de Medicina i Cirurgia Animals, Facultat de veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra SpainInstitute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Parc Científic, E-08028 Barcelona, SpainDept. Bioquímica i Biomedicina Molecular. Facultat de Biologia. Universitat de Barcelona, E-08028 Barcelona, SpainDept. de Medicina i Cirurgia Animals, Facultat de veterinària, Universitat Autònoma de Barcelona, E-08193 Bellaterra SpainDept. Bioquímica i Biomedicina Molecular. Facultat de Biologia. Universitat de Barcelona, E-08028 Barcelona, SpainThe aim of the study reported herein was to evaluate the suitability of the Surface Acoustic Wave (SAW) technique as a possible diagnostic tool in benign prostatic hyperplasia (BPH). Moreover, for the first time, the BPH model was a totally physiological using naturally aged rats with spontaneous, age-related BPH instead of the pharmacologically induced models usually used. Eighteen male Wistar rats were distributed according to their age: 6 weeks (young), 12 weeks (adult) and 12 months (old) old. Prostate gland was removed and analyzed by mini-arrays, Western blotting (WB) and SAW techniques. Mini-arrays indicated that there were significant differences in the expression of 29/34 inflammation-related cytokines. WB was carried out to confirm the results after selection of 4 cytokines from which one showed no changes, namely PDGF-AA, and the other three, which significantly increase in older animals, were CD86, β-NGF and VEGF. Notwithstanding, WB of old rats yielded confusing results due to an anomalous migration of proteins, dismissing this technique as an useful tool in these animals. Accurate results in old rats were uniquely obtained by using the SAW technique. Thus, SAW analysis showed that there were not differences among groups in the amount of PDGF-AA. On the contrary, SAW analysis showed that amounts of CD86, β-NGF and VEGF in old rats were 2.0, 1.9 and 5.7-fold higher than that from young ones, respectively. These results indicate that SAW is a highly accurate technique for determining changes in the cytokines expression in BPH.http://www.sciencedirect.com/science/article/pii/S2405580817301723Benign age-related prostatic hyperplasiaInflammatory cytokinesSurface Acoustic Wave technique |
| spellingShingle | Maria M. Rivera del Alamo Mireia Díaz-Lobo Silvia Busquets Joan E. Rodríguez-Gil Josep M. Fernández-Novell Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats Biochemistry and Biophysics Reports Benign age-related prostatic hyperplasia Inflammatory cytokines Surface Acoustic Wave technique |
| title | Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats |
| title_full | Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats |
| title_fullStr | Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats |
| title_full_unstemmed | Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats |
| title_short | Specific expression pattern of tissue cytokines analyzed through the Surface Acoustic Wave technique is associated with age-related spontaneous benign prostatic hyperplasia in rats |
| title_sort | specific expression pattern of tissue cytokines analyzed through the surface acoustic wave technique is associated with age related spontaneous benign prostatic hyperplasia in rats |
| topic | Benign age-related prostatic hyperplasia Inflammatory cytokines Surface Acoustic Wave technique |
| url | http://www.sciencedirect.com/science/article/pii/S2405580817301723 |
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