<it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2
<p>Abstract</p> <p>Background</p> <p>The pathogenicity of <it>Plasmodium falciparum </it>is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell receptors and serum-proteins. Binding of the pRBC is mediate...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-01-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/10/1/17 |
| _version_ | 1818233077427601408 |
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| author | Chen Qijun Normark Johan Blomqvist Karin Moll Kirsten Albrecht Letusa Wahlgren Mats |
| author_facet | Chen Qijun Normark Johan Blomqvist Karin Moll Kirsten Albrecht Letusa Wahlgren Mats |
| author_sort | Chen Qijun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The pathogenicity of <it>Plasmodium falciparum </it>is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell receptors and serum-proteins. Binding of the pRBC is mediated by <it>Plasmodium falciparum </it>erythrocyte membrane protein 1 (PfEMP1), a large multi-variant molecule encoded by a family of ≈60 <it>var </it>genes.</p> <p>Methods</p> <p>The study of <it>var </it>gene transcription in the parasite clone FCR3S1.2 was performed by semi-quantitative PCR and quantitative PCR (qPCR). The expression of the major PfEMP1 in FCR3S1.2 pRBC was analysed with polyclonal sera in rosette disruption assays and immunofluorecence.</p> <p>Results</p> <p>Transcripts from <it>var</it>1 (FCR3S1.2<sub><it>var</it></sub><sub>1</sub>; IT4<it>var</it>21) and other <it>var </it>genes were detected by semi-quantitative PCR but results from qPCR showed that one <it>var </it>gene transcript dominated over the others (FCR3S1.2<sub><it>var</it></sub><sub>2</sub>; IT4<it>var</it>60). Antibodies raised in rats to the recombinant NTS-DBL1α of <it>var</it>2 produced in <it>E. coli </it>completely and dose-dependently disrupted rosettes (≈95% at a dilution of 1/5). The sera reacted with the Maurer's clefts in trophozoite stages (IFA) and to the infected erythrocyte surface (FACS) indicating that FCR3S1.2<sub><it>var2 </it></sub>encodes the dominant PfEMP1 expressed in this parasite.</p> <p>Conclusion</p> <p>The major transcript in the rosetting model parasite FCR3S1.2 is FCR3S1.2<sub><it>var</it></sub><sub>2 </sub>(IT4<it>var</it>60). The results suggest that this gene encodes the PfEMP1-species responsible for the rosetting phenotype of this parasite. The activity of previously raised antibodies to the NTS-DBL1α of FCR3S1.2<sub><it>var</it></sub><sub>1 </sub>is likely due to cross-reactivity with NTS-DBL1α of the <it>var</it>2 encoded PfEMP1.</p> |
| first_indexed | 2024-12-12T11:16:27Z |
| format | Article |
| id | doaj.art-f7c325e2b07340e7a98d0274d8badaa0 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-12T11:16:27Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
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| series | Malaria Journal |
| spelling | doaj.art-f7c325e2b07340e7a98d0274d8badaa02022-12-22T00:26:08ZengBMCMalaria Journal1475-28752011-01-011011710.1186/1475-2875-10-17<it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2Chen QijunNormark JohanBlomqvist KarinMoll KirstenAlbrecht LetusaWahlgren Mats<p>Abstract</p> <p>Background</p> <p>The pathogenicity of <it>Plasmodium falciparum </it>is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell receptors and serum-proteins. Binding of the pRBC is mediated by <it>Plasmodium falciparum </it>erythrocyte membrane protein 1 (PfEMP1), a large multi-variant molecule encoded by a family of ≈60 <it>var </it>genes.</p> <p>Methods</p> <p>The study of <it>var </it>gene transcription in the parasite clone FCR3S1.2 was performed by semi-quantitative PCR and quantitative PCR (qPCR). The expression of the major PfEMP1 in FCR3S1.2 pRBC was analysed with polyclonal sera in rosette disruption assays and immunofluorecence.</p> <p>Results</p> <p>Transcripts from <it>var</it>1 (FCR3S1.2<sub><it>var</it></sub><sub>1</sub>; IT4<it>var</it>21) and other <it>var </it>genes were detected by semi-quantitative PCR but results from qPCR showed that one <it>var </it>gene transcript dominated over the others (FCR3S1.2<sub><it>var</it></sub><sub>2</sub>; IT4<it>var</it>60). Antibodies raised in rats to the recombinant NTS-DBL1α of <it>var</it>2 produced in <it>E. coli </it>completely and dose-dependently disrupted rosettes (≈95% at a dilution of 1/5). The sera reacted with the Maurer's clefts in trophozoite stages (IFA) and to the infected erythrocyte surface (FACS) indicating that FCR3S1.2<sub><it>var2 </it></sub>encodes the dominant PfEMP1 expressed in this parasite.</p> <p>Conclusion</p> <p>The major transcript in the rosetting model parasite FCR3S1.2 is FCR3S1.2<sub><it>var</it></sub><sub>2 </sub>(IT4<it>var</it>60). The results suggest that this gene encodes the PfEMP1-species responsible for the rosetting phenotype of this parasite. The activity of previously raised antibodies to the NTS-DBL1α of FCR3S1.2<sub><it>var</it></sub><sub>1 </sub>is likely due to cross-reactivity with NTS-DBL1α of the <it>var</it>2 encoded PfEMP1.</p>http://www.malariajournal.com/content/10/1/17 |
| spellingShingle | Chen Qijun Normark Johan Blomqvist Karin Moll Kirsten Albrecht Letusa Wahlgren Mats <it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2 Malaria Journal |
| title | <it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2 |
| title_full | <it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2 |
| title_fullStr | <it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2 |
| title_full_unstemmed | <it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2 |
| title_short | <it>var </it>gene transcription and PfEMP1 expression in the rosetting and cytoadhesive <it>Plasmodium falciparum </it>clone FCR3S1.2 |
| title_sort | it var it gene transcription and pfemp1 expression in the rosetting and cytoadhesive it plasmodium falciparum it clone fcr3s1 2 |
| url | http://www.malariajournal.com/content/10/1/17 |
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