A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular Localization
Viruses infecting bacteria (bacteriophages) represent the most abundant viral particles in the human body. They participate in the control of the human-associated bacterial communities and play an important role in the dissemination of virulence genes. Here, we present the identification of a new fi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/3/475 |
| _version_ | 1797239068249030656 |
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| author | Nikolay Popgeorgiev Mart Krupovic Julien Hiblot Laura Fancello Sonia Monteil-Bouchard Christelle Desnues |
| author_facet | Nikolay Popgeorgiev Mart Krupovic Julien Hiblot Laura Fancello Sonia Monteil-Bouchard Christelle Desnues |
| author_sort | Nikolay Popgeorgiev |
| collection | DOAJ |
| description | Viruses infecting bacteria (bacteriophages) represent the most abundant viral particles in the human body. They participate in the control of the human-associated bacterial communities and play an important role in the dissemination of virulence genes. Here, we present the identification of a new filamentous single-stranded DNA phage of the family <i>Inoviridae</i>, named Ralstonia Inoviridae Phage 1 (RIP1), in the human blood. Metagenomics and PCR analyses detected the RIP1 genome in blood serum, in the absence of concomitant bacterial infection or contamination, suggesting inovirus persistence in the human blood. Finally, we have experimentally demonstrated that the RIP1-encoded rolling circle replication initiation protein and serine integrase have functional nuclear localization signals and upon expression in eukaryotic cells both proteins were translocated into the nucleus. This observation adds to the growing body of data suggesting that phages could have an overlooked impact on the evolution of eukaryotic cells. |
| first_indexed | 2024-04-24T17:45:39Z |
| format | Article |
| id | doaj.art-f7c75a6a7f354c37845501e6d85eb513 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-24T17:45:39Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f7c75a6a7f354c37845501e6d85eb5132024-03-27T14:08:00ZengMDPI AGViruses1999-49152024-03-0116347510.3390/v16030475A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular LocalizationNikolay Popgeorgiev0Mart Krupovic1Julien Hiblot2Laura Fancello3Sonia Monteil-Bouchard4Christelle Desnues5Université de Lyon, Centre de Recherche en Cancérologie de Lyon, U1052 INSERM, UMR CNRS 5286, Université Lyon I, Centre Léon Bérard, 28 rue Laennec, 69008 Lyon, FranceArchaeal Virology Unit, Institut Pasteur, Université Paris Cité, 75015 Paris, FranceDepartment of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, GermanyInterdisciplinary Research Institute of Grenoble, IRIG-Biosanté, University Grenoble Alpes, CEA, INSERM, UMR 1292, 38000 Grenoble, FranceMicrobiologie Environnementale Biotechnologie, Institut Méditerranéen d’Océanologie, 163 Avenue de Luminy, 13009 Marseille, FranceMicrobiologie Environnementale Biotechnologie, Institut Méditerranéen d’Océanologie, 163 Avenue de Luminy, 13009 Marseille, FranceViruses infecting bacteria (bacteriophages) represent the most abundant viral particles in the human body. They participate in the control of the human-associated bacterial communities and play an important role in the dissemination of virulence genes. Here, we present the identification of a new filamentous single-stranded DNA phage of the family <i>Inoviridae</i>, named Ralstonia Inoviridae Phage 1 (RIP1), in the human blood. Metagenomics and PCR analyses detected the RIP1 genome in blood serum, in the absence of concomitant bacterial infection or contamination, suggesting inovirus persistence in the human blood. Finally, we have experimentally demonstrated that the RIP1-encoded rolling circle replication initiation protein and serine integrase have functional nuclear localization signals and upon expression in eukaryotic cells both proteins were translocated into the nucleus. This observation adds to the growing body of data suggesting that phages could have an overlooked impact on the evolution of eukaryotic cells.https://www.mdpi.com/1999-4915/16/3/475inovirusesmetagenomicsnuclear localization |
| spellingShingle | Nikolay Popgeorgiev Mart Krupovic Julien Hiblot Laura Fancello Sonia Monteil-Bouchard Christelle Desnues A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular Localization Viruses inoviruses metagenomics nuclear localization |
| title | A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular Localization |
| title_full | A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular Localization |
| title_fullStr | A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular Localization |
| title_full_unstemmed | A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular Localization |
| title_short | A New Inovirus from the Human Blood Encodes Proteins with Nuclear Subcellular Localization |
| title_sort | new inovirus from the human blood encodes proteins with nuclear subcellular localization |
| topic | inoviruses metagenomics nuclear localization |
| url | https://www.mdpi.com/1999-4915/16/3/475 |
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