Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese Patients
The goal of our study was to determine the expression and phosphorylation of PTEN at residues Ser380/Thr382/383, as well as the nuclear expression of p53 in Sudanese patients with breast cancer (BC) in association with clinicopathological aspects of BC. Methods and Results: This retrospective, desc...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2023-03-01
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| Series: | International Journal of Biomedicine |
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| Online Access: | http://www.ijbm.org/articles/i49/ijbm_13(1)_oa7.pdf |
| _version_ | 1811160067104309248 |
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| author | Abubaker M Hamad Rania Mahjoub Ser Alkhatem Asaad K Algahany Hussain G Ahmed Abdulaziz Alfahed Hisham Ali Waggiallah |
| author_facet | Abubaker M Hamad Rania Mahjoub Ser Alkhatem Asaad K Algahany Hussain G Ahmed Abdulaziz Alfahed Hisham Ali Waggiallah |
| author_sort | Abubaker M Hamad |
| collection | DOAJ |
| description | The goal of our study was to determine the expression and phosphorylation of PTEN at residues Ser380/Thr382/383, as well as the nuclear expression of p53 in Sudanese patients with breast cancer (BC) in association with clinicopathological aspects of BC.
Methods and Results: This retrospective, descriptive study was carried out in Wad-Madani, Gezira state in Sudan, from January 2015 to August 2016. A total of 179 biopsies were taken at random from patients with breast lesions. Two blocks were obtained for each patient. One came from a malignant lesion (Group A), whereas the other came from the margin adjacent to healthy tissue (Group B). Immunohistochemistry and immunofluorescent histochemistry were performed on two separate slides. We found a statistically significant difference in the frequency of immunohistochemical expression of p53 and phosphorylation of PTEN between the cancerous breast tissue and adjacent normal tissue. P53 and PTEN exhibited a significant relationship with each other and the grade of tumor, indicating their importance in the aggressiveness of breast lesions. It should also be emphasized that there is an association between p53 expression and lymph node metastasis, which indicates the involvement of p53 mutation in the metastasis of BC.
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| first_indexed | 2024-04-10T05:51:22Z |
| format | Article |
| id | doaj.art-f7c856ef020c4222821317e5c272b63d |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-04-10T05:51:22Z |
| publishDate | 2023-03-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-f7c856ef020c4222821317e5c272b63d2023-03-05T00:31:26ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292023-03-01131626810.21103/Article13(1)_OA7Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese PatientsAbubaker M Hamad0Rania Mahjoub Ser Alkhatem1Asaad K Algahany2Hussain G Ahmed3Abdulaziz Alfahed4Hisham Ali Waggiallah5College of Health Sciences, AL-Rayan Colleges, Madinah Al Munawwarah, Kingdom of Saudi Arabia; Department of Histopathology and Cytopathology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Madani, SudanDepartment of Histopathology and Cytopathology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Madani, SudanDepartment of Pathology, College of Medicine, University of Hail, Hail, Saudi ArabiaDepartment of Pathology, College of Medicine, University of Hail, Hail, Saudi Arabia; Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, SudanDepartment of Medical Laboratory Science, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi ArabiaDepartment of Medical Laboratory Science, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi ArabiaThe goal of our study was to determine the expression and phosphorylation of PTEN at residues Ser380/Thr382/383, as well as the nuclear expression of p53 in Sudanese patients with breast cancer (BC) in association with clinicopathological aspects of BC. Methods and Results: This retrospective, descriptive study was carried out in Wad-Madani, Gezira state in Sudan, from January 2015 to August 2016. A total of 179 biopsies were taken at random from patients with breast lesions. Two blocks were obtained for each patient. One came from a malignant lesion (Group A), whereas the other came from the margin adjacent to healthy tissue (Group B). Immunohistochemistry and immunofluorescent histochemistry were performed on two separate slides. We found a statistically significant difference in the frequency of immunohistochemical expression of p53 and phosphorylation of PTEN between the cancerous breast tissue and adjacent normal tissue. P53 and PTEN exhibited a significant relationship with each other and the grade of tumor, indicating their importance in the aggressiveness of breast lesions. It should also be emphasized that there is an association between p53 expression and lymph node metastasis, which indicates the involvement of p53 mutation in the metastasis of BC. http://www.ijbm.org/articles/i49/ijbm_13(1)_oa7.pdfptenp53breast cancerimmunohistochemistry |
| spellingShingle | Abubaker M Hamad Rania Mahjoub Ser Alkhatem Asaad K Algahany Hussain G Ahmed Abdulaziz Alfahed Hisham Ali Waggiallah Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese Patients International Journal of Biomedicine pten p53 breast cancer immunohistochemistry |
| title | Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese Patients |
| title_full | Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese Patients |
| title_fullStr | Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese Patients |
| title_full_unstemmed | Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese Patients |
| title_short | Expression of P53 and PTEN in Correlation with some Clinical and Pathological Features in Breast Cancer of Sudanese Patients |
| title_sort | expression of p53 and pten in correlation with some clinical and pathological features in breast cancer of sudanese patients |
| topic | pten p53 breast cancer immunohistochemistry |
| url | http://www.ijbm.org/articles/i49/ijbm_13(1)_oa7.pdf |
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