Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation
While dysbiosis within the intestinal ecosystem has been associated with functional constipation (FC), the mechanisms underlying the interactions between FC and the microbiome remain poorly elucidated. Recent investigations suggested that host microRNAs (miRNAs) can modulate bacterial growth and inf...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1323877/full |
| _version_ | 1797357750082076672 |
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| author | Junpeng Yao Xiangyun Yan Yanqiu Li Yaoyao Chen Xianjun Xiao Siyuan Zhou Wei Zhang Lu Wang Min Chen Fang Zeng Ying Li |
| author_facet | Junpeng Yao Xiangyun Yan Yanqiu Li Yaoyao Chen Xianjun Xiao Siyuan Zhou Wei Zhang Lu Wang Min Chen Fang Zeng Ying Li |
| author_sort | Junpeng Yao |
| collection | DOAJ |
| description | While dysbiosis within the intestinal ecosystem has been associated with functional constipation (FC), the mechanisms underlying the interactions between FC and the microbiome remain poorly elucidated. Recent investigations suggested that host microRNAs (miRNAs) can modulate bacterial growth and influence the composition of the gut microbiome. To explore the connection between gut microbiota and fecal miRNAs in FC patients, we initially employed 16S rRNA sequencing to assess the gut microbial landscape in 30 FC patients and 30 healthy controls (HCs). The α-diversity within the FC group exhibited some alterations, and the β-diversity significantly differed, signifying distinctive variations in gut microbiota composition between FC patients and HCs. Subsequently, we identified 44 differentially expressed (DE) miRNAs in feces from FC patients and HCs. Through correlation analysis between DE miRNAs and FC-associated microbiota, we detected an interaction involving nine DE miRNAs (miR-205-5p, miR-493-5p, miR-215-5p, miR-184, miR-378c, miR-335-5p, miR-514a-3p, miR-141-3p, and miR-34c-5p) with seven bacterial genera (Oscillibacter, Escherichia.Shigella, UCG.002, Lachnospiraceae_NK4A136_group, Lachnospiraceae_UCG.010, Eubacterium_ruminantium_group and Megamonas), as evidenced by a co-occurrence network. Further, a comprehensive panel of seven diagnostic biomarkers (Oscillibacter, Escherichia.Shigella, UCG.002, miR-205-5p, miR-493-5p, miR-215-5p, and Lachnospiraceae_NK4A136_group) demonstrated robust discriminatory capacity in predicting FC status when integrated into a random forest model (AUC = 0.832, 95% CI: 65.73–98.88). Microbiomes correlating with DE miRNAs exhibited enrichment in distinct predicted metabolic categories. Moreover, miRNAs correlated with FC-associated bacteria were found to be enriched in signaling pathways linked to colonic contractility, including Axon guidance, PI3K-Akt signaling pathway, MAPK signaling pathway, and Hippo signaling pathway. Our study offers a comprehensive insight into the global relationship between microbiota and fecal miRNAs in the context of FC, presenting potential targets for further experimental validation and therapeutic interventions. |
| first_indexed | 2024-03-08T14:49:32Z |
| format | Article |
| id | doaj.art-f7cfc18b7e704db0a6e681cd2af97b6e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T14:49:32Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f7cfc18b7e704db0a6e681cd2af97b6e2024-01-11T05:09:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011410.3389/fmicb.2023.13238771323877Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipationJunpeng Yao0Xiangyun Yan1Yanqiu Li2Yaoyao Chen3Xianjun Xiao4Siyuan Zhou5Wei Zhang6Lu Wang7Min Chen8Fang Zeng9Ying Li10Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaSchool of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaTeaching Affairs Office, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaDepartment of Colorectal Disease, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaWhile dysbiosis within the intestinal ecosystem has been associated with functional constipation (FC), the mechanisms underlying the interactions between FC and the microbiome remain poorly elucidated. Recent investigations suggested that host microRNAs (miRNAs) can modulate bacterial growth and influence the composition of the gut microbiome. To explore the connection between gut microbiota and fecal miRNAs in FC patients, we initially employed 16S rRNA sequencing to assess the gut microbial landscape in 30 FC patients and 30 healthy controls (HCs). The α-diversity within the FC group exhibited some alterations, and the β-diversity significantly differed, signifying distinctive variations in gut microbiota composition between FC patients and HCs. Subsequently, we identified 44 differentially expressed (DE) miRNAs in feces from FC patients and HCs. Through correlation analysis between DE miRNAs and FC-associated microbiota, we detected an interaction involving nine DE miRNAs (miR-205-5p, miR-493-5p, miR-215-5p, miR-184, miR-378c, miR-335-5p, miR-514a-3p, miR-141-3p, and miR-34c-5p) with seven bacterial genera (Oscillibacter, Escherichia.Shigella, UCG.002, Lachnospiraceae_NK4A136_group, Lachnospiraceae_UCG.010, Eubacterium_ruminantium_group and Megamonas), as evidenced by a co-occurrence network. Further, a comprehensive panel of seven diagnostic biomarkers (Oscillibacter, Escherichia.Shigella, UCG.002, miR-205-5p, miR-493-5p, miR-215-5p, and Lachnospiraceae_NK4A136_group) demonstrated robust discriminatory capacity in predicting FC status when integrated into a random forest model (AUC = 0.832, 95% CI: 65.73–98.88). Microbiomes correlating with DE miRNAs exhibited enrichment in distinct predicted metabolic categories. Moreover, miRNAs correlated with FC-associated bacteria were found to be enriched in signaling pathways linked to colonic contractility, including Axon guidance, PI3K-Akt signaling pathway, MAPK signaling pathway, and Hippo signaling pathway. Our study offers a comprehensive insight into the global relationship between microbiota and fecal miRNAs in the context of FC, presenting potential targets for further experimental validation and therapeutic interventions.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1323877/fullfunctional constipationgut microbiotamicroRNA16S rRNAmicroRNA sequencing |
| spellingShingle | Junpeng Yao Xiangyun Yan Yanqiu Li Yaoyao Chen Xianjun Xiao Siyuan Zhou Wei Zhang Lu Wang Min Chen Fang Zeng Ying Li Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation Frontiers in Microbiology functional constipation gut microbiota microRNA 16S rRNA microRNA sequencing |
| title | Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation |
| title_full | Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation |
| title_fullStr | Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation |
| title_full_unstemmed | Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation |
| title_short | Altered gut microbial profile is associated with differentially expressed fecal microRNAs in patients with functional constipation |
| title_sort | altered gut microbial profile is associated with differentially expressed fecal micrornas in patients with functional constipation |
| topic | functional constipation gut microbiota microRNA 16S rRNA microRNA sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1323877/full |
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