Diagnostic Properties of Three SARS-CoV-2 Antibody Tests
Serological assays emerged as complementary tools to RT-PCR in the diagnosis of SARS-CoV-2 as well as being needed for epidemiological studies. This study aimed to assess the performance of a rapid test (RT) compared to that of serological tests using finger prick blood samples. A total of 183 sampl...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/8/1441 |
| _version_ | 1797524209105108992 |
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| author | Suelen Basgalupp Giovana dos Santos Marina Bessel Lara Garcia Ana Carolina de Moura Ana Carolina Rocha Emerson Brito Giovana de Miranda Thayane Dornelles William Dartora Lucia Pellanda Pedro Hallal Eliana Wendland |
| author_facet | Suelen Basgalupp Giovana dos Santos Marina Bessel Lara Garcia Ana Carolina de Moura Ana Carolina Rocha Emerson Brito Giovana de Miranda Thayane Dornelles William Dartora Lucia Pellanda Pedro Hallal Eliana Wendland |
| author_sort | Suelen Basgalupp |
| collection | DOAJ |
| description | Serological assays emerged as complementary tools to RT-PCR in the diagnosis of SARS-CoV-2 as well as being needed for epidemiological studies. This study aimed to assess the performance of a rapid test (RT) compared to that of serological tests using finger prick blood samples. A total of 183 samples were evaluated, 88 of which were collected from individuals with negative RT-PCR and 95 from positive RT-PCR individuals. The diagnostic performance of RT (WONDFO<sup>®</sup>) and LUMIT (PROMEGA<sup>®</sup>) were compared to that of ELISA (EUROIMMUN<sup>®</sup>) for detecting antibodies against SARS-CoV-2 according to time from symptoms onset. The IgG antibody tests were detected in 77.4% (LUMIT), 77.9% (RT), and 80.0% (ELISA) of individuals. The detection of antibodies against SARS-CoV-2 increases in accordance with increasing time from symptoms onset. Considering only time from symptoms onset >21 days, the positivity rate ranged from 81.8 to 97.0% between the three tests. The RT and LUMIT showed high agreement with ELISA (agreement = 91.5%, k = 0.83, and agreement = 96.3%, k = 0.9, respectively) in individuals who had symptoms 15 to 21 days before sample collection. Compared to that of the ELISA assay, our results show sensitivity ranged from 95% to 100% for IgG antibody detection in individuals with symptoms onset between 15 and 21 days before sample collection. The specificity was 100% in individuals with symptoms onset >15 days before serological tests. This study shows good performance and high level of agreement of three immunoassays for the detection of SARS-CoV-2 antibodies. |
| first_indexed | 2024-03-10T08:54:08Z |
| format | Article |
| id | doaj.art-f7d3b9389e0c4539a79c3003fce3ae2c |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T08:54:08Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-f7d3b9389e0c4539a79c3003fce3ae2c2023-11-22T07:20:33ZengMDPI AGDiagnostics2075-44182021-08-01118144110.3390/diagnostics11081441Diagnostic Properties of Three SARS-CoV-2 Antibody TestsSuelen Basgalupp0Giovana dos Santos1Marina Bessel2Lara Garcia3Ana Carolina de Moura4Ana Carolina Rocha5Emerson Brito6Giovana de Miranda7Thayane Dornelles8William Dartora9Lucia Pellanda10Pedro Hallal11Eliana Wendland12Hospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilDepartment of Public Health, Universidade Federal de Ciências da Saúde, de Porto Alegre, Porto Alegre 90050-170, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilDepartment of Public Health, Universidade Federal de Ciências da Saúde, de Porto Alegre, Porto Alegre 90050-170, RS, BrazilPostgraduate Programme in Epidemiology, Universidade Federal de Pelotas, Pelotas 96020-220, RS, BrazilHospital Moinhos de Vento, Porto Alegre 90035-000, RS, BrazilSerological assays emerged as complementary tools to RT-PCR in the diagnosis of SARS-CoV-2 as well as being needed for epidemiological studies. This study aimed to assess the performance of a rapid test (RT) compared to that of serological tests using finger prick blood samples. A total of 183 samples were evaluated, 88 of which were collected from individuals with negative RT-PCR and 95 from positive RT-PCR individuals. The diagnostic performance of RT (WONDFO<sup>®</sup>) and LUMIT (PROMEGA<sup>®</sup>) were compared to that of ELISA (EUROIMMUN<sup>®</sup>) for detecting antibodies against SARS-CoV-2 according to time from symptoms onset. The IgG antibody tests were detected in 77.4% (LUMIT), 77.9% (RT), and 80.0% (ELISA) of individuals. The detection of antibodies against SARS-CoV-2 increases in accordance with increasing time from symptoms onset. Considering only time from symptoms onset >21 days, the positivity rate ranged from 81.8 to 97.0% between the three tests. The RT and LUMIT showed high agreement with ELISA (agreement = 91.5%, k = 0.83, and agreement = 96.3%, k = 0.9, respectively) in individuals who had symptoms 15 to 21 days before sample collection. Compared to that of the ELISA assay, our results show sensitivity ranged from 95% to 100% for IgG antibody detection in individuals with symptoms onset between 15 and 21 days before sample collection. The specificity was 100% in individuals with symptoms onset >15 days before serological tests. This study shows good performance and high level of agreement of three immunoassays for the detection of SARS-CoV-2 antibodies.https://www.mdpi.com/2075-4418/11/8/1441SARS-CoV-2COVID-19serological testsantibodiespoint-of-care testELISA |
| spellingShingle | Suelen Basgalupp Giovana dos Santos Marina Bessel Lara Garcia Ana Carolina de Moura Ana Carolina Rocha Emerson Brito Giovana de Miranda Thayane Dornelles William Dartora Lucia Pellanda Pedro Hallal Eliana Wendland Diagnostic Properties of Three SARS-CoV-2 Antibody Tests Diagnostics SARS-CoV-2 COVID-19 serological tests antibodies point-of-care test ELISA |
| title | Diagnostic Properties of Three SARS-CoV-2 Antibody Tests |
| title_full | Diagnostic Properties of Three SARS-CoV-2 Antibody Tests |
| title_fullStr | Diagnostic Properties of Three SARS-CoV-2 Antibody Tests |
| title_full_unstemmed | Diagnostic Properties of Three SARS-CoV-2 Antibody Tests |
| title_short | Diagnostic Properties of Three SARS-CoV-2 Antibody Tests |
| title_sort | diagnostic properties of three sars cov 2 antibody tests |
| topic | SARS-CoV-2 COVID-19 serological tests antibodies point-of-care test ELISA |
| url | https://www.mdpi.com/2075-4418/11/8/1441 |
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