Evaluating Helicobacter Pylori FlaA in Clinical Isolates from Alzahra Hospital, Isfahan, Iran
Background: This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori. Methods: One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain react...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2013-04-01
|
| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2565 |
| Summary: | Background: This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori.
Methods: One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain reaction (PCR) was performed using the designed primer.
Findings: PCR products showed that all extracted DNA were positive for flaA gene.
Conclusion: We found flaA gene to be present in all clinical isolates. As it is highly similar to standard Helicobacter pylori strains, it can be used to detect the mentioned bacteria. |
|---|---|
| ISSN: | 1027-7595 1735-854X |